Anti-PTDSS1 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTDSS1 antibody (AB237019)

Immunohistochemical analysis of ab237019 diluted at 1 : 150 and staining in paraffin-embedded humanpancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labelled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody

• WB

Supplier Data

Western blot - Anti-PTDSS1 antibody (AB237019)

All lanes:

Western blot - Anti-PTDSS1 antibody (ab237019) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 20 µg

Lane 2:

K562 whole cell lysate at 20 µg

Lane 3:

HepG2 whole cell lysate at 20 µg

Lane 4:

MCF7 whole cell lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/20000 dilution

Observed band size: 35 kDa

false

Exposure time: 60s