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AB290640

Anti-PTP zeta/ Phosphacan antibody [EPR26270-86]

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(1 리뷰)

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(1 출판물)

Rabbit Recombinant Monoclonal PTP zeta/ Phosphacan antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

대체 명칭 보기

HTPZP2, PTPRZ, PTPRZ2, PTPZ, PTPRZ1, Receptor-type tyrosine-protein phosphatase zeta, R-PTP-zeta, Protein-tyrosine phosphatase receptor type Z polypeptide 1, Protein-tyrosine phosphatase receptor type Z polypeptide 2, R-PTP-zeta-2

8 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)

Immunohistochemical analysis of paraffin-embedded human liver tissue labelling PTP zeta/ Phosphacan with ab290640 at 1/2000 (0.259 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) . Negative control : no staining on human liver. The section was incubated with ab290640 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) .

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)

Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labelling PTP zeta/ Phosphacan with ab290640 at 1/2000 (0.259 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on human astrocytoma. The section was incubated with ab290640 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling PTP zeta/ Phosphacan with ab290640 at 1/2000 (0.259 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on human cerebrum. The section was incubated with ab290640 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling PTP zeta/ Phosphacan with ab290640 at 1/2000 (0.259 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) . Positive staining on rat cerebrum. The section was incubated with ab290640 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) .

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling PTP zeta/ Phosphacan with ab290640 at 1/2000 (0.259 μg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) . Positive staining on mouse cerebrum. The section was incubated with ab290640 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) .

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)
  • WB

Supplier Data

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)

Blocking and diluting buffer and concentration was 5% NFDM/TBST.

Several bands in the range from 380 to 70 kDa were observed. The profile is consistent with what has been described in the literature (PMID : 18713734).

Negative control : liver, U-87 MG(PMID : 7477903, 23170925).

All lanes:

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human spinal cord tissue lysate at 20 µg

Lane 4:

NCI-H69 (human small cell lung cancer ) whole cell lysate at 20 µg

Lane 5:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 255 kDa

Observed band size: 380-70 kDa

false

Exposure time: 3min

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)
  • WB

Supplier Data

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)

Blocking and diluting buffer and concentration was 5% NFDM/TBST.

After chondroitinase ABC treatment, several bands in the range from 380 to 70 kDa were observed. The profile is consistent with what has been described in the literature (PMID : 18713734).

Negative control : liver(PMID : 7477903).

All lanes:

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640) at 1/1000 dilution

Lane 1:

Mouse P14 brain tissue lysate at 20 µg

Lane 2:

Mouse P14 brain tissue lysate treated with 0.05U chondroitinase ABC for 3h at 37℃ at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 255 kDa

Observed band size: 380-70 kDa

false

Exposure time: 48s

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)
  • WB

Supplier Data

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (AB290640)

Blocking and diluting buffer and concentration was 5% NFDM/TBST.

PTPRZ gene generates three isoforms by alternative splicing : two transmembrane isoforms PTPRZ-A and PTPRZ-B, and one secretory isoform PTPRZ-S which is also known as phosphacan. All three isoforms expressed in the brain are highly glycosylated with chondroitin sulfate. After chondroitinase ABC treatment, several bands in the range from 380 to 70 kDa were observed. The profile is consistent with what has been described in the literature (PMID : 18713734).

Negative control : liver(PMID : 7477903).

All lanes:

Western blot - Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] (ab290640) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate treated with 0.05U chondroitinase ABC for 3h at 37℃ at 20 µg

Lane 3:

Rat liver tissue lysate at 20 µg

Lane 4:

Rat spinal cord tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 255 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-PTP zeta/ Phosphacan antibody [EPR26270-86] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26270-86

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB277525

Anti-Neurocan antibody [EPR23425-68]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurocan antibody [EPR23425-68] (AB277525)

  • 0
  • 0
Primary Antibodies

AB285162

Anti-Brevican antibody [EPR25743-59]

BOND RX™ Validated|RabMAb|20ul selling size|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brevican antibody [EPR25743-59] (AB285162)

  • 0
  • 0
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 76
Primary Antibodies

AB278054

Anti-GFAP antibody [RM1003] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [RM1003] - Astrocyte Marker (AB278054)

  • 0
  • 0
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Reactivity 정보

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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PTP zeta also known as Phosphacan is a protein tyrosine phosphatase expressed mainly in the central nervous system. It is a chondroitin sulfate proteoglycan with a molecular weight of approximately 340 kDa. This protein is found on neuronal and glial cell surfaces. PTP zeta plays a role in cell signaling by dephosphorylating specific tyrosine residues on target proteins. Its interaction with ECM molecules and receptors supports cell-cell communication and structural organization within neural tissues.
Biological function summary

In development and recovery of nervous system components PTP zeta plays key roles. It engages in signaling networks that regulate cell adhesion migration and proliferation. Phosphacan shares structures with other receptor-like proteins and interfaces with various ligands indicating its involvement in complex multi-protein assemblies. Interaction with growth factors such as pleiotrophin influences neural cell differentiation and axonal growth.

Pathways

PTP zeta integrates into signaling processes controlling neural plasticity and regeneration. It participates in the PI3K/Akt pathway where it modifies cell survival and growth dynamics. The protein also associates with the MAPK/ERK pathway impacting neuronal differentiation and proliferation. Here it interacts with other proteins like fibroblast growth factor (FGF) enhancing its regulatory influence on cellular functions critical to the CNS.

PTP zeta has connections with conditions like glioblastoma and schizophrenia. Altered expression levels associate it with these pathologies as it significantly influences cell proliferation and migration patterns that could contribute to tumor growth or neurodevelopmental anomalies. PTP zeta interacts with integrin proteins which are implicated in mediating these disease mechanisms through their role in ECM and cell cytoskeleton interactions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Protein tyrosine phosphatase that negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. Required for normal differentiation of the precursor cells into mature, fully myelinating oligodendrocytes. May play a role in protecting oligondendrocytes against apoptosis. May play a role in the establishment of contextual memory, probably via the dephosphorylation of proteins that are part of important signaling cascades (By similarity).
See full target information PTPRZ1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

ACS omega 10:17584-17594 PubMed40352542

2025

Remote Ischemic Postconditioning Protects the Neurovascular Units in MCAO/R Rats through HIF-1α-Mediated Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Haocheng Qin,Lu Sun,Lu Su,Bao Zhou,Zhong He,Pengkun Yang,Zhengran Ding,Yu-Lian Zhu,Dan Liang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

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