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AB62330

Anti-PTP1B (phospho S352) antibody [EP1839Y]

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Rabbit Monoclonal PTP1B phospho S352 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.
1 이미지
Western blot - Anti-PTP1B (phospho S352) antibody [EP1839Y] (AB62330)
  • WB

Unknown

Western blot - Anti-PTP1B (phospho S352) antibody [EP1839Y] (AB62330)

All lanes:

Western blot - Anti-PTP1B (phospho S352) antibody [EP1839Y] (ab62330) at 1/5000 dilution

Lane 1:

Hela cell lysates (untreated) at 10 µg

Lane 2:

Hela cell lysates (treated with Calyculin A) at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-PTP1B (phospho S352) antibody [EP1839Y] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1839Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB244207

Anti-PTP1B antibody [EPR22474]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EPR22474] (AB244207)

  • 0
  • 0
Primary Antibodies

AB76239

Anti-PTP1B (phospho S378) antibody [EP1840Y]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B (phospho S378) antibody [EP1840Y] (AB76239)

  • 0
  • 0
Primary Antibodies

AB232050

Anti-PTP1B antibody [EPR1836(2)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-PTP1B antibody [EPR1836(2)] - BSA and Azide free (AB232050)

  • 0
  • 0
Primary Antibodies

AB133244

Anti-PTP1B antibody [EPR1836(2)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-PTP1B antibody [EPR1836(2)] (AB133244)

  • 5
  • 1
Primary Antibodies

AB245984

Anti-PTP1B antibody [EPR22474] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EPR22474] - BSA and Azide free (AB245984)

  • 0
  • 0
Primary Antibodies

AB75856

Anti-PTP1B antibody [EP1841Y]

RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Anti-PTP1B antibody [EP1841Y] (AB75856)

  • 0
  • 0
Primary Antibodies

AB238956

Anti-PTP1B antibody [EP1841Y] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-PTP1B antibody [EP1841Y] - BSA and Azide free (AB238956)

  • 0
  • 0

보완 제품

Primary Antibodies

AB62320

Anti-PTP1B (phospho S50) antibody [EP1838Y]

RabMAb

Western blot - Anti-PTP1B (phospho S50) antibody [EP1838Y] (AB62320)

  • 0
  • 0
Primary Antibodies

AB76239

Anti-PTP1B (phospho S378) antibody [EP1840Y]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTP1B (phospho S378) antibody [EP1840Y] (AB76239)

  • 0
  • 0
Primary Antibodies

AB244207

Anti-PTP1B antibody [EPR22474]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PTP1B antibody [EPR22474] (AB244207)

  • 0
  • 0
Primary Antibodies

AB180764

Anti-TCPTP antibody

Immunocytochemistry/ Immunofluorescence - Anti-TCPTP antibody (AB180764)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500 - 1/5000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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특성 및 보관 정보

제형
Liquid
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Protein tyrosine phosphatase 1B (PTP1B) is an enzyme involved in the dephosphorylation of tyrosine residues in proteins. Known also as PTPN1 this protein has a molecular weight of approximately 50 kDa. PTP1B is widely expressed in tissues but is highly present in the liver muscle and adipose tissue. It plays a critical role in regulating signal transduction pathways by removing phosphate groups from phosphotyrosine residues on target proteins. This action generally serves as a 'turn-off' switch for various signaling pathways.
Biological function summary

PTP1B plays important roles in glucose and lipid metabolism. It is not generally considered a part of a complex functioning instead as a singular enzyme. Its main action involves the negative regulation of the insulin signaling pathway by dephosphorylating the insulin receptor as well as its associated substrates. This regulation helps maintain normal levels of insulin sensitivity influencing cellular glucose uptake and metabolism.

Pathways

PTP1B has important functions in the insulin and leptin signaling pathways. It has a regulatory influence in these pathways affecting key metabolic processes. In the insulin signaling pathway PTP1B closely interacts with the insulin receptor as well as with insulin receptor substrates (IRS). Its dephosphorylating activity can lead to decreased insulin sensitivity. In the leptin pathway PTP1B modulates leptin signaling by dephosphorylating the Janus kinase 2 (JAK2) another protein involved in the control of energy balance and metabolism.

Excessive activity of PTP1B links with disorders such as type 2 diabetes and obesity. Overactivity of PTP1B can lead to insulin resistance contributing to the pathogenesis of type 2 diabetes. In obesity PTP1B may interfere with the normal activity of leptin a hormone critical for regulating energy balance. Its interaction with the insulin receptor and JAK2 makes PTP1B a target for therapeutic interventions aimed at improving insulin sensitivity and energy homeostasis.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
See full target information PTPN1 pS352

대체 명칭 보기

PTP1B, PTPN1, Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death and differentiation : PubMed40841483

2025

BRCA1 and BRCA2 gene expression: p53- and cell cycle-dependent repression requires RB and DREAM.

Applications

Unspecified application

Species

Unspecified reactive species

Marianne Quaas,Robin Kohler,Lukas Nöltner,Louisa F Schmidbauer,Sigrid Uxa,Gerd A Müller,Kurt Engeland
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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