Anti-PTPN14 antibody [EPR30487-534] - BSA and Azide free

• WB

Lab

Western blot - Anti-PTPN14 antibody [EPR30487-534] - BSA and Azide free (AB326108)

This data was developed using ab326098, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : 293T(PMID : 10934049)

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) 1 : 10000 (124Kda).

All lanes:

Western blot - Anti-PTPN14 antibody [EPR30487-534] (<a href='/ko/products/primary-antibodies/ptpn14-antibody-epr30487-534-ab326098'>ab326098</a>) at 1/1000 dilution

Lane 1:

DU 145 (human prostate carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 80 µg

Lane 2:

DU 145 transfected with siRNA specifically targeting PTPN14 whole cell lysate at 80 µg

Lane 3:

293T (human embryonic kidney epithelial cell) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 135 kDa,124 kDa

false

Exposure time: 135s

• WB

Lab

Western blot - Anti-PTPN14 antibody [EPR30487-534] - BSA and Azide free (AB326108)

This data was developed using ab326098, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : small intestine

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lane 1 : 26 seconds, lane 2 : 180 seconds

All lanes:

Western blot - Anti-PTPN14 antibody [EPR30487-534] (<a href='/ko/products/primary-antibodies/ptpn14-antibody-epr30487-534-ab326098'>ab326098</a>) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 80 µg

Lane 2:

Mouse small intestine tissue lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 135 kDa,36 kDa

false

• Dot

Lab

Dot Blot - Anti-PTPN14 antibody [EPR30487-534] - BSA and Azide free (AB326108)

This data was developed using ab326098, the same antibody clone in a different buffer formulation.

Dot blot analysis of PTPN14 using ab326098 at 1 : 1000 (0.515 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : His-tagged human PTPN14 recombinant protein fragment

Lane 2 : LIF-tagged human PTPN21 recombinant protein fragment

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human PTPN21.

All lanes:

Dot Blot - Anti-PTPN14 antibody [EPR30487-534] (<a href='/ko/products/primary-antibodies/ptpn14-antibody-epr30487-534-ab326098'>ab326098</a>) at 1/1000 dilution

Lane 1:

His-tagged human PTPN14 recombinant protein fragment

Lane 2:

LIF-tagged human PTPN21 recombinant protein fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s