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AB326376

Anti-PTPRS antibody [EPR30833-525] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal PTPRS antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-PTPRS antibody [EPR30833-525] - BSA and Azide free (Detector) (AB326376)
  • sELISA

Lab

Sandwich ELISA - Anti-PTPRS antibody [EPR30833-525] - BSA and Azide free (Detector) (AB326376)

Interpolated concentration of native PTPRS was measured at different sample concentrations. Undiluted samples are as follows : SH-SY5Y cell extract (200 µg/mL) and SK-N-BE(2) cell extract (100 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-PTPRS antibody [EPR30833-525] - BSA and Azide free (Detector) (AB326376)
  • sELISA

Lab

Sandwich ELISA - Anti-PTPRS antibody [EPR30833-525] - BSA and Azide free (Detector) (AB326376)

Interpolated concentration of native PTPRS was measured at different sample concentrations. Undiluted sample is as follows : human plasma (EDTA) 100%. Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-PTPRS antibody [EPR30833-525] - BSA and Azide free (Detector) (AB326376)
  • sELISA

Lab

Sandwich ELISA - Anti-PTPRS antibody [EPR30833-525] - BSA and Azide free (Detector) (AB326376)

Sandwich ELISA with the capture antibody ab326375 dilution at 2 µg/mL and detector antibody ab326376 dilution at 0.5 µg/mL after biotin conjugation.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30833-525

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific to human PTPRS and shows no cross-reactivity with human PTPRD, or PTPRF. The antibody pair, consisting of ab326375 as the capture and ab326376 as the detector, exhibits no cross-reactivity with human PTPRD, or PTPRF.

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보완 제품

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab326376 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PTPRS. The recommended pair for sandwich ELISA is ab326375, Human PTPRS Capture Antibody (unconjugated)Detector: ab326376, Human PTPRS Detector Antibody (unconjugated)The reference range value is 15.6-1000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Cell surface receptor that binds to glycosaminoglycans, including chondroitin sulfate proteoglycans and heparan sulfate proteoglycan (PubMed : 21454754). Binding to chondroitin sulfate and heparan sulfate proteoglycans has opposite effects on PTPRS oligomerization and regulation of neurite outgrowth. Contributes to the inhibition of neurite and axonal outgrowth by chondroitin sulfate proteoglycans, also after nerve transection. Plays a role in stimulating neurite outgrowth in response to the heparan sulfate proteoglycan GPC2. Required for normal brain development, especially for normal development of the pituitary gland and the olfactory bulb. Functions as a tyrosine phosphatase (PubMed : 8524829). Mediates dephosphorylation of NTRK1, NTRK2 and NTRK3 (By similarity). Plays a role in down-regulation of signaling cascades that lead to the activation of Akt and MAP kinases (By similarity). Down-regulates TLR9-mediated activation of NF-kappa-B, as well as production of TNF, interferon alpha and interferon beta (PubMed : 26231120).
See full target information PTPRS

대체 명칭 보기

Receptor-type tyrosine-protein phosphatase S, R-PTP-S, Receptor-type tyrosine-protein phosphatase sigma, R-PTP-sigma, PTPRS
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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