AB183327

Anti-PU.1/Spi1 antibody [SP238]

20ul selling size
Recombinant
Advanced Validation
RabMAb
각 태그의 뜻

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(5 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal PU.1/Spi1 antibody. Suitable for IHC-P, Flow Cyt (Intra), ICC/IF, ChIC/CUT&RUN-seq and reacts with Human samples. Cited in 5 publications.

대체 명칭 보기

Transcription factor PU.1, 31 kDa-transforming protein, SPI1

6 이미지

Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD23 with purified ab135386 at 1/25 (7.48 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488 ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cytometry (Intracellular) - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

Flow Cytometry analysis of THP-1 (human monocytic leukemia monocyte) cells labeling PU.1/Spi1 with purified ab183327 at 1 : 200 dilution (0.715 μg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling PU.1/Spi1 with ab183327 at 1/100.

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 THP-1 (Human monocytic leukemia monocyte) cells and 5 µg of ab183327 [SP238]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."

ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP238

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ChIC/CUT&RUN-seq, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2000", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "", "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "" } } }

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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

Purified from TCS by protein A/G.

보관 버퍼

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PU.1 also known as Spi1 is a transcription factor that belongs to the ETS family of transcription factors. It plays a major role in gene regulation and is an important player in hematopoietic cell differentiation. PU.1 has a molecular mass of approximately 31 kDa and is predominantly expressed in hematopoietic cells such as myeloid and B-lymphoid cells. It actively interacts with specific DNA sequences to regulate the expression of target genes.

Biological function summary

PU.1 influences the development and function of various blood cells including macrophages neutrophils and B-cells. It is essential for the regulation of genes involved in immune responses cell proliferation and survival. PU.1 functions as part of a larger transcriptional regulatory complex and often partners with other transcription factors and coactivators to exert its effects. This cooperation allows precise gene expression control in specific cell lineages.

Pathways

Expression of PU.1 is critical in the hematopoietic development pathway and the immune response pathway. It closely interacts with other transcription factors like GATA-1 and C/EBPα forming a network that affects hematopoietic lineage commitment. Within these pathways PU.1 constantly coordinates signals that influence progenitor cell fate and differentiation ensuring a balanced proportion of cell types within the blood.

PU.1 has significant implications in leukemia and other hematological malignancies. Dysregulation or mutation of PU.1 can lead to improper hematopoietic cell development and contribute to acute myeloid leukemia (AML) and other blood disorders. Additionally its expression level is closely linked with immune system function associating PU.1 indirectly with autoimmune conditions. In these contexts the interaction with other proteins like AML1 and C/EBPγ can influence disease pathogenesis by modulating gene expression patterns critical for normal hematopoietic and immune function.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed : 23658224, PubMed : 33951726). Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity). Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity). May be particularly important for the pro- to pre-B cell transition (PubMed : 33951726). Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed : 33951726). In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).

See full target information SPI1

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of neuroinflammation 21:163 PubMed38918792

2024

Maternal SARS-CoV-2 impacts fetal placental macrophage programs and placenta-derived microglial models of neurodevelopment.

Applications

Unspecified application

Species

Unspecified reactive species

Lydia L Shook,Rebecca E Batorsky,Rose M De Guzman,Liam T McCrea,Sara M Brigida,Joy E Horng,Steven D Sheridan,Olha Kholod,Aidan M Cook,Jonathan Z Li,Donna K Slonim,Brittany A Goods,Roy H Perlis,Andrea G Edlow

HemaSphere 8:e32 PubMed38434534

2024

CDK6 protein expression is associated with disease progression and treatment resistance in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Steinhart,Peter Möller,Miriam Kull,Jan Krönke,Thomas F E Barth

Biological psychiatry 95:676-686 PubMed37573007

2023

Loss of Function in the Neurodevelopmental Disease and Schizophrenia-Associated Gene CYFIP1 in Human Microglia-like Cells Supports a Functional Role in Synaptic Engulfment.

Applications

Unspecified application

Species

Unspecified reactive species

Steven D Sheridan,Joy E Horng,Hana Yeh,Liam McCrea,Jennifer Wang,Ting Fu,Roy H Perlis

Translational psychiatry 11:179 PubMed33741894

2021

Umbilical cord blood-derived microglia-like cells to model COVID-19 exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Steven D Sheridan,Jessica M Thanos,Rose M De Guzman,Liam T McCrea,Joy E Horng,Ting Fu,Carl M Sellgren,Roy H Perlis,Andrea G Edlow

bioRxiv : the preprint server for biology : PubMed33052344

2020

Umbilical cord blood derived microglia-like cells to model COVID-19 exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Steven D Sheridan,Jessica M Thanos,Rose M De Guzman,Liam T McCrea,Joy Horng,Ting Fu,Carl M Sellgren,Roy H Perlis,Andrea G Edlow

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PU.1/Spi1 antibody [SP238]

Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

Flow Cytometry (Intracellular) - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (5)

Product promise