AB226340

Anti-PYCR1 antibody - C-terminal

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(3 제품이 사용된 논문 )

Rabbit Polyclonal PYCR1 antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Pyrroline-5-carboxylate reductase 1, mitochondrial aa 250-350.

대체 명칭 보기

P5C reductase 1, P5CR 1, PYCR1

2 이미지

Supplier Data

Immunoprecipitation - Anti-PYCR1 antibody - C-terminal (AB226340)

PYCR1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226340 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226340 at 1 μg/ml.

Lane 1 : ab226340 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-PYCR1 antibody - C-terminal (ab226340)

Predicted band size: 33 kDa

false

Supplier Data

Western blot - Anti-PYCR1 antibody - C-terminal (AB226340)

Cell lysates were prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-PYCR1 antibody - C-terminal (ab226340) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 33 kDa

true

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human Pyrroline-5-carboxylate reductase 1, mitochondrial aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

P32322

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab226340 was affinity purified using an epitope specific to PYCR1 immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PYCR1 also known as Pyrroline-5-carboxylate reductase 1 is an enzyme with a molecular weight of approximately 33 kDa. It facilitates the conversion of pyrroline-5-carboxylate to proline an essential amino acid. PYCR1 is mainly expressed in human tissues with high metabolic activity such as the liver kidney and pancreas which highlights its role in amino acid metabolism. This enzyme plays a central role in maintaining the balance between cellular oxidation and reduction and is typically active in the cytoplasm of the cells.

Biological function summary

PYCR1 participates in the proline biosynthesis pathway and contributes to the cellular response to stress. It functions as a homopolymer indicating its need to form complexes for activity. The production of proline is important as proline protects cells by maintaining membrane integrity and stabilizing proteins during environmental stress like oxidative stress. Proline synthesis involving PYCR1 is essential for collagen production which supports tissue and structural integrity in humans.

Pathways

PYCR1 acts within the proline biosynthesis pathway and the larger metabolism of amino acids. This enzyme works closely with ALDH4A1 which is another enzyme that catalyzes the earlier stages of the proline biosynthesis pathway before PYCR1 performs its function. The pathway plays a significant role in plant and animal cell adaptation to environmental changes. By modulating these pathways cells gain enhanced abilities to manage osmotic pressures and support vital functions under stress conditions linking PYCR1's activity to cellular survival and resilience.

PYCR1 mutations or dysfunction is associated with Cutis laxa a rare connective tissue disorder characterized by loose sagging skin and systemic involvement. These dysfunctions affect proline synthesis impairing collagen production which leads to abnormal connective tissue structure. PYCR1 is also connected to pyrroline-5-carboxylate synthetase implicating dysfunction in the larger metabolic framework that supports protein synthesis and cellular health. These disturbances underline the enzyme's importance in maintaining normal physiological conditions and hint at possible therapeutic targets for related disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate to L-proline using NAD(P)H (PubMed : 16730026, PubMed : 19648921, PubMed : 23024808, PubMed : 28258219). At physiologic concentrations, has higher specific activity in the presence of NADH (PubMed : 16730026, PubMed : 23024808). Involved in the cellular response to oxidative stress (PubMed : 16730026, PubMed : 19648921).

See full target information Pyrroline-5-carboxylate reductase 1, mitochondrial

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Alzheimer's research & therapy 17:100 PubMed40336092

2025

Soluble SorLA in CSF, a novel biomarker to explore disrupted trafficking of SorLA protein in Alzheimer disease.

Applications

Unspecified application

Species

Unspecified reactive species

Romain Castelot,Aline Zarea,David Wallon,Anne Rovelet-Lecrux,Catherine Schramm,Muriel Quillard-Muraine,Anne Beaume,Frédéric Blanc,Olivier Bousiges,Julien Dumurgier,Maïté Formaglio,Gwenael Leguyader,Sylvain Lehmann,Cecilia Marelli,Matthieu Martinet,Leonor Nogueira,Jérémie Pariente,Isabelle Quadrio,Adeline Rollin-Sillaire,Susanna Schraen,Gaël Nicolas,Magalie Lecourtois

The journal of gene medicine 25:e3527 PubMed37382425

2023

Antisense lncRNA-RP11-498C9.13 promotes bladder cancer progression by enhancing reactive oxygen species-induced mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Song,Zhuo Li,Ke Yang,Zhiyong Gao,Qiang Zhou,Ping Li

Aging 12: PubMed33461172

2021

Dysregulation of USP18/FTO/PYCR1 signaling network promotes bladder cancer development and progression.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Song,Ke Yang,Jianjun Luo,Zhiyong Gao,Yunliang Gao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PYCR1 antibody - C-terminal

Immunoprecipitation - Anti-PYCR1 antibody - C-terminal (AB226340)

Western blot - Anti-PYCR1 antibody - C-terminal (AB226340)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise