AB126224

Anti-Pyruvate Dehydrogenase E2 antibody

Name: Anti-Pyruvate Dehydrogenase E2 antibody
Brand: Abcam Limited
SKU: AB126224
Rating: 5 (1 reviews)

(1 리뷰)

(5 제품이 사용된 논문 )

Rabbit Polyclonal Pyruvate Dehydrogenase E2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DLAT aa 300-550.

대체 명칭 보기

DLTA, DLAT, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, M2 antigen complex 70 kDa subunit, Pyruvate dehydrogenase complex component E2, PBC, PDC-E2, PDCE2

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Pyruvate Dehydrogenase E2 antibody (AB126224)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pyruvate Dehydrogenase E2 antibody (AB126224)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling DLAT protein at mitochondria using ab126224 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

CiteAb

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody (AB126224)

Pyruvate Dehydrogenase E2 western blot using anti-Pyruvate Dehydrogenase E2 antibody ab126224. Publication image and figure legend from Osinalde, N., Sánchez-Quiles, V., et al., 2016, Sci Rep, PubMed 27025927.

ab126224 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab126224 please see the product overview.

IL-2-inducible Gab2-interacting proteins.(A) Heat map and hierarchical clustering dendrogram indicating the SILAC IL-2/Ctr ratio displayed by the cytokine-inducible Gab2-binding protein in each replica. (B) Validation of selected well-known and novel Gab2-binding proteins by immunoblot. (C) Relative binding abundance of the detected Gab2 interactors. *indicates described Gab2 interactors.

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DLAT aa 300-550. The exact immunogen used to generate this antibody is proprietary information.

P10515

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Pyruvate Dehydrogenase E2 (PDH-E2) also referred to as PDC-E2 or E2 protein is a central component of cellular metabolism. It is the inner core dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex. PDH-E2 facilitates the conversion of pyruvate to acetyl-CoA which is an important step in aerobic respiration. The E2 protein is expressed in the mitochondria where it actively engages in its enzymatic roles. With a molecular weight of approximately 55 kDa PDH-E2 functions through its interaction with other components of the pyruvate dehydrogenase complex.

Biological function summary

PDH-E2 plays a significant role in cellular energy production. It is an important part of the pyruvate dehydrogenase complex where its coordinated enzymatic activity drives the decarboxylation of pyruvate. This activity supplies acetyl-CoA for the citric acid cycle essential for ATP production. PDH-E2's function regulates the flow of carbon within cells linking glycolysis and the citric acid cycle making it essential for energy homeostasis. Its function integrates signals that regulate metabolic pathways helping maintain cellular energy balance.

Pathways

PDH-E2 integrates the glycolysis and citric acid cycle facilitating efficient energy production. It interacts with tightly linked enzymes in the pyruvate dehydrogenase complex important for catalyzing the conversion processes. Key related proteins include pyruvate dehydrogenase (E1) and dihydrolipoamide dehydrogenase (E3) which form the complex alongside E2. This complex is also critical in adjusting metabolic responses based on cellular energy needs efficiently managing substrate flux into the mitochondria.

Dysfunctional PDH-E2 activity associates with metabolic conditions like pyruvate dehydrogenase deficiency and primary biliary cholangitis (PBC). PDH deficiency leads to lactic acidosis and neurological dysfunction due to impaired energy production. In PBC the immune system aberrantly targets the E2 component causing liver damage and leading to cholestasis. These diseases highlight the critical role of PDH-E2 and its interaction with other mitochondrial proteins in maintaining metabolic health.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

The pyruvate dehydrogenase (PDH) complex, catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (Probable). It contains multiple copies of three enzymatic components : pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3); (Probable). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acetyl groups from acetyl-lipoyl moiety generated by the pyruvate dehydrogenase, leading to acetyl-CoA formation (Probable).

See full target information DLAT

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 15:4244 PubMed38762605

2024

Mitochondrial respiratory function is preserved under cysteine starvation via glutathione catabolism in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan P Ward,Sang Jun Yoon,Tyce Flynn,Amanda M Sherwood,Maddison A Olley,Juliana Madej,Gina M DeNicola

International journal of molecular sciences 24: PubMed37761999

2023

Association of Phosphorylated Pyruvate Dehydrogenase with Pyruvate Kinase M2 Promotes PKM2 Stability in Response to Insulin.

Applications

Unspecified application

Species

Unspecified reactive species

Abu Jubayer Hossain,Rokibul Islam,Jong-Bok Seo,Hwee-Seon Park,Jong-Il Kim,Vikas Kumar,Keun Woo Lee,Jae-Bong Park

Biomedicines 10: PubMed35740278

2022

Pyruvate Dehydrogenase A1 Phosphorylated by Insulin Associates with Pyruvate Kinase M2 and Induces LINC00273 through Histone Acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Abu Jubayer Hossain,Rokibul Islam,Jae-Gyu Kim,Oyungerel Dogsom,Kim Cuong Cap,Jae-Bong Park

The Journal of experimental medicine 217: PubMed32196080

2020

Nicotinamide nucleotide transhydrogenase regulates mitochondrial metabolism in NSCLC through maintenance of Fe-S protein function.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan P Ward,Yun Pyo Kang,Aimee Falzone,Theresa A Boyle,Gina M DeNicola

Scientific reports 6:23530 PubMed27025927

2016

Changes in Gab2 phosphorylation and interaction partners in response to interleukin (IL)-2 stimulation in T-lymphocytes.

Applications

Species

Unspecified reactive species

Nerea Osinalde,Virginia Sánchez-Quiles,Blagoy Blagoev,Irina Kratchmarova

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pyruvate Dehydrogenase E2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Pyruvate Dehydrogenase E2 antibody (AB126224)

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody (AB126224)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (5)

Product promise