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AB240168

Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Pyruvate Dehydrogenase E2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, Transfected cell lysate samples. Cited in 1 publication.

대체 명칭 보기

DLTA, DLAT, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, M2 antigen complex 70 kDa subunit, Pyruvate dehydrogenase complex component E2, PBC, PDC-E2, PDCE2

15 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)

This data was developed using ab172617, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cervical cancer labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human cervical cancer.
The section was incubated with ab172617 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)

This data was developed using ab172617, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cerebrum labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human cerebrum.
The section was incubated with ab172617 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)

This data was developed using ab172617, the same antibody clone in a different buffer formulation.

ab172617 staining Pyruvate Dehydrogenase E2 in A549 (positive) and A549-DLAT (negative) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated for 2 hours at room temperature with ab172617 at 1 ug/ml (shown in green) and ab7291, Anti-alpha Tubulin antibody [DM1A] - Loading Control (shown in pseudocolour magenta). Cells were then incubated with ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution and ab150119, Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution for 1 hour at room temperature. Nuclear DNA was labeled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min). Image was acquired with a high-content analyser (Operetta CLS, Revvity) and a maximum intensity projection of confocal sections is shown.

Flow Cytometry (Intracellular) - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172617).

Immunocytochemistry/ Immunofluorescence - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)

Immunofluorescent analysis of HepG2 cells labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172617).

Immunoprecipitation - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)
  • IP

Supplier Data

Immunoprecipitation - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)

This data was developed using ab172617, the same antibody clone in a different buffer formulation.

Immunoprecipitation of DLAT in A549 cells. Lysates were prepared and immunoprecipitation was performed using 2 μg of ab172617 pre-coupled to Protein A beads. Samples were then washed and processed for western blot.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Immunoprecipitation - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (<a href='/ko/products/primary-antibodies/pyruvate-dehydrogenase-e2-antibody-epr12455b-ab172617'>ab172617</a>) at 2 µg

All lanes:

A549 cells lysates

Observed band size: 68 kDa

false

Immunoprecipitation - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)
  • IP

Supplier Data

Immunoprecipitation - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)

Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab172617 at 1/10 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172617).

All lanes:

Immunoprecipitation - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (<a href='/ko/products/primary-antibodies/pyruvate-dehydrogenase-e2-antibody-epr12455b-ab172617'>ab172617</a>)

Predicted band size: 69 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)

This data was developed using ab172617, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat kidney labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on rat kidney.
The section was incubated with ab172617 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)

This data was developed using ab172617, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse kidney labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on mouse kidney.
The section was incubated with ab172617 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)
  • WB

Supplier Data

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)

This data was developed using ab172617, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (<a href='/ko/products/primary-antibodies/pyruvate-dehydrogenase-e2-antibody-epr12455b-ab172617'>ab172617</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Predicted band size: 69 kDa

false

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)
  • WB

Supplier Data

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)

This data was developed using ab172617, the same antibody clone in a different buffer formulation.

ab172617 was shown to react with DLAT in wild-type A549 cells in Western blot with loss of signal observed in DLAT knockout cell line ab300919. Wild-type A549 and DLAT knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab172617 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (<a href='/ko/products/primary-antibodies/pyruvate-dehydrogenase-e2-antibody-epr12455b-ab172617'>ab172617</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 lysate at 30 µg

Lane 2:

DLAT knock-out A549 lysate at 30 µg

Lane 2:

Western blot - Human DLAT knockout A549 cell line (<a href='/ko/products/cell-lines/human-dlat-knockout-a549-cell-line-ab300919'>ab300919</a>)

Observed band size: 68 kDa

false

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)
  • WB

Lab

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)

This data was developed using ab172617, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (<a href='/ko/products/primary-antibodies/pyruvate-dehydrogenase-e2-antibody-epr12455b-ab172617'>ab172617</a>) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 2:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 69 kDa

false

Exposure time: 48s

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)
  • WB

Lab

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)

This data was developed using ab172617, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (<a href='/ko/products/primary-antibodies/pyruvate-dehydrogenase-e2-antibody-epr12455b-ab172617'>ab172617</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 69 kDa

false

Exposure time: 15s

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)
  • WB

Lab

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)

This data was developed using ab172617, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (<a href='/ko/products/primary-antibodies/pyruvate-dehydrogenase-e2-antibody-epr12455b-ab172617'>ab172617</a>) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

NIH/3T3 transfected with siRNA specifically targeting Dlat-1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 69 kDa

false

Exposure time: 15s

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)
  • WB

Lab

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] - BSA and Azide free (AB240168)

This data was developed using ab172617, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (<a href='/ko/products/primary-antibodies/pyruvate-dehydrogenase-e2-antibody-epr12455b-ab172617'>ab172617</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 69 kDa

false

Exposure time: 26s

관련 conjugated 항체와 다양한 조성의 항체 (5)

  • Unconjugated

    Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)]

  • 660 APC

    APC Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)]

  • 578 PE

    PE Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12455(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB126224

Anti-Pyruvate Dehydrogenase E2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Pyruvate Dehydrogenase E2 antibody (AB126224)

  • 5
  • 1
Primary Antibodies

AB110332

Anti-Pyruvate Dehydrogenase E2 antibody [15D3G9C11]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Pyruvate Dehydrogenase E2 antibody [15D3G9C11] (AB110332)

  • 0
  • 0

보완 제품

Assay Kits

AB109902

Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit

Functional Studies - Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit (AB109902)

  • 3
  • 4
Primary Antibodies

AB110330

Anti-PDHA1 antibody [9H9AF5]

KO Validated

Western blot - Anti-PDHA1 antibody [9H9AF5] (AB110330)

  • 5
  • 8
Primary Antibodies

AB110333

Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5]

KO Validated

Western blot - Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5] (AB110333)

  • 4
  • 6
Primary Antibodies

AB250066

Anti-DLST antibody [EPR13319(B)] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLST antibody [EPR13319(B)] - BSA and Azide free (AB250066)

  • 0
  • 0
Primary Antibodies

AB155996

Anti-PDHB antibody [EPR11097(B)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (AB155996)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Transfected cell lysate": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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제품 세부 정보

ab240168 is the carrier-free version of ab172617.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Pyruvate Dehydrogenase E2 (PDH-E2) also referred to as PDC-E2 or E2 protein is a central component of cellular metabolism. It is the inner core dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex. PDH-E2 facilitates the conversion of pyruvate to acetyl-CoA which is an important step in aerobic respiration. The E2 protein is expressed in the mitochondria where it actively engages in its enzymatic roles. With a molecular weight of approximately 55 kDa PDH-E2 functions through its interaction with other components of the pyruvate dehydrogenase complex.
Biological function summary

PDH-E2 plays a significant role in cellular energy production. It is an important part of the pyruvate dehydrogenase complex where its coordinated enzymatic activity drives the decarboxylation of pyruvate. This activity supplies acetyl-CoA for the citric acid cycle essential for ATP production. PDH-E2's function regulates the flow of carbon within cells linking glycolysis and the citric acid cycle making it essential for energy homeostasis. Its function integrates signals that regulate metabolic pathways helping maintain cellular energy balance.

Pathways

PDH-E2 integrates the glycolysis and citric acid cycle facilitating efficient energy production. It interacts with tightly linked enzymes in the pyruvate dehydrogenase complex important for catalyzing the conversion processes. Key related proteins include pyruvate dehydrogenase (E1) and dihydrolipoamide dehydrogenase (E3) which form the complex alongside E2. This complex is also critical in adjusting metabolic responses based on cellular energy needs efficiently managing substrate flux into the mitochondria.

Dysfunctional PDH-E2 activity associates with metabolic conditions like pyruvate dehydrogenase deficiency and primary biliary cholangitis (PBC). PDH deficiency leads to lactic acidosis and neurological dysfunction due to impaired energy production. In PBC the immune system aberrantly targets the E2 component causing liver damage and leading to cholestasis. These diseases highlight the critical role of PDH-E2 and its interaction with other mitochondrial proteins in maintaining metabolic health.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

The pyruvate dehydrogenase (PDH) complex, catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (Probable). It contains multiple copies of three enzymatic components : pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3); (Probable). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acetyl groups from acetyl-lipoyl moiety generated by the pyruvate dehydrogenase, leading to acetyl-CoA formation (Probable).
See full target information DLAT
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 43:494-500 PubMed37147750

2023

Electroacupuncture alleviates water avoidance stress-induced irritable bowel syndrome in mice by improving intestinal barrier functions and suppressing the expression of inflammatory cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Sun Mengzhu,Zhang Yujie,Song Yafang,Guo Jing,Wang Yuhang,Xin Chen,G U Dongmei,Sun Jianhua,Pei Lixia
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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