JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB237600

Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Recombinant Monoclonal Quiescin Q6 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

QSCN6, UNQ2520/PRO6013, QSOX1, Sulfhydryl oxidase 1, hQSOX, Quiescin Q6

4 이미지
Flow Cytometry (Intracellular) - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (AB237600)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (AB237600)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Quiescin Q6 with ab235444 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235444).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (AB237600)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (AB237600)

Immunohistochemical analysis of paraffin-embedded human breast ductal cancer tissue labeling Quiescin Q6 with ab235444 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in human breast ductal cancer (PMID : 23536962) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235444).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (AB237600)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (AB237600)

Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling Quiescin Q6 with ab235444 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in human pancreatic cancer (PMID : 19795908, 21989104) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235444).

Immunoprecipitation - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (AB237600)
  • IP

Unknown

Immunoprecipitation - Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (AB237600)

Quiescin Q6 was immunoprecipitated from 0.35 mg of MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate with ab235444 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235444 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : MDA-MB-231 whole cell lysate 10 μg (Input).
Lane 2 : ab235444 IP in MDA-MD-231 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab235444 in MDA-MD-231 whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 30 seconds.

Quiescin Q6 has two isoforms due to expression from a long (Quiescin Q6-L) and a short (Quiescin Q6-S) transcript.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235444).

All lanes:

Immunoprecipitation - Anti-Quiescin Q6 antibody [EPR21866] (<a href='/ko/products/primary-antibodies/quiescin-q6-antibody-epr21866-ab235444'>ab235444</a>)

Predicted band size: 83 kDa

Observed band size: 65 kDa,83 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21866

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

보완 제품

Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB77924

Anti-Parkin antibody [PRK8]

KO Validated

Western blot - Anti-Parkin antibody [PRK8] (AB77924)

  • 4
  • 12
Primary Antibodies

AB46545

Anti-4 Hydroxynonenal antibody

Western blot - Anti-4 Hydroxynonenal antibody (AB46545)

  • 4
  • 27
Primary Antibodies

AB216144

Anti-PINK1 antibody [EPR20730]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR20730] (AB216144)

  • 4
  • 4
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

ab237600 is the carrier-free version of ab235444.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Quiescin Q6 also known as QSOX1 is an enzyme belonging to the Quiescin-sulfhydryl oxidase family. It functions by catalyzing the oxidation of sulfhydryl groups to form disulfide bonds in proteins an important step for proper protein folding. QSOX1 has a molecular mass between 60-100 kDa depending on its isoform. This protein is expressed in various tissues with high levels present in the pancreas placenta and some cancer cells suggesting a role in tissue specific processes and during certain pathological conditions.
Biological function summary

Quiescin Q6 influences cell growth and differentiation. The protein is involved in extracellular matrix organization where it plays a role in maintaining the stability and function of connective tissues. It doesn't typically assemble into large complexes but interacts with substrates directly facilitating the formation of stable proteins. QSOX1 ensures that proteins achieve their functional conformation by reshaping their disulfide bonds efficiently aiding in the cellular machinery of protein conformation.

Pathways

The functions of Quiescin Q6 intersect with the protein folding and oxidative stress response pathways. It relates closely to the endoplasmic reticulum protein folding pathway due to its role in disulfide bonding as well as to the antioxidant defense pathway where it mediates protection against oxidative stress. Its activity connects with proteins such as protein disulfide isomerase (PDI) which also participate in creating disulfide bonds but at different stages or cellular locations showcasing distinct but cooperative functions within these pathways.

Quiescin Q6 has been linked to cancer progression and fibrosis. Its overexpression has been observed in various tumors indicating a potential role in tumor growth and metastasis. In fibrotic diseases elevated levels of QSOX1 contribute to excessive extracellular matrix deposition. It connects with the transforming growth factor-beta (TGF-beta) pathway a critical regulator in fibrosis and cancer where QSOX1's regulation of matrix composition may influence TGF-beta signaling. Understanding these connections offers potential for developing targeted therapeutic strategies in treating related diseases.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide (PubMed : 17331072, PubMed : 18393449, PubMed : 23704371, PubMed : 23867277, PubMed : 30367560). Plays a role in disulfide bond formation in a variety of extracellular proteins (PubMed : 17331072, PubMed : 22801504, PubMed : 23867277, PubMed : 30367560). In fibroblasts, required for normal incorporation of laminin into the extracellular matrix, and thereby for normal cell-cell adhesion and cell migration (PubMed : 23704371, PubMed : 23867277, PubMed : 30367560).
See full target information QSOX1
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in behavioral neuroscience 17:1172091 PubMed37273278

2023

Resveratrol ameliorates maternal separation-induced anxiety- and depression-like behaviors and reduces Sirt1-NF-kB signaling-mediated neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ru-Meng Wei,Yue-Ming Zhang,Yi-Zhou Feng,Kai-Xuan Zhang,Jing-Ya Zhang,Jing Chen,Bao-Ling Luo,Xue-Yan Li,Gui-Hai Chen
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com