AB244307

Anti-RAB antibody

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Rabbit Polyclonal RAB antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AGFG1 aa 200-350.

대체 명칭 보기

HRB, RAB, RIP, AGFG1, Arf-GAP domain and FG repeat-containing protein 1, HIV-1 Rev-binding protein, Nucleoporin-like protein RIP, Rev-interacting protein, Rev/Rex activation domain-binding protein

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB antibody (AB244307)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB antibody (AB244307)

Formalin-fixed, paraffin-embedded human placenta tissue stained for RAB using ab244307 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RAB antibody (AB244307)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RAB antibody (AB244307)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for RAB (green) using ab244307 at 4 μg/ml in ICC/IF.

Unknown

Western blot - Anti-RAB antibody (AB244307)

All lanes:

Western blot - Anti-RAB antibody (ab244307) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 58 kDa

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Unknown

Western blot - Anti-RAB antibody (AB244307)

All lanes:

Western blot - Anti-RAB antibody (ab244307) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (rat Wistar bladder tumor cell line) cell lysate

Predicted band size: 58 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human AGFG1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

P52594

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The RAB proteins also known as RAS-related proteins are small GTPases with a molecular weight of approximately 20–25 kDa. They belong to the Ras superfamily which are key regulators of intracellular trafficking. RAB proteins primarily localize to specific intracellular membranes where they direct vesicle movement docking and fusion. Different RAB proteins express in distinct tissues reflecting their specific roles in cell function. For example certain RAB proteins predominantly express in the brain while others are found in muscle or epithelial tissues.

Biological function summary

Small GTPases like RAB proteins operate as molecular switches in the cell. They participate in processes such as endocytosis exocytosis and other vesicle transport mechanisms. RAB proteins often work in conjunction with other members of the GTPase family and can assemble into regulatory complexes on vesicle membranes. These complexes control the specificity and timing of vesicle trafficking events ensuring precise delivery of cellular cargo.

Pathways

Small GTPases such as RAB proteins integrate into several key cellular signaling and trafficking pathways. They are vital components of the vesicular transport pathways including the endocytic and secretory pathways. RAB proteins communicate with effector proteins like the SNAREs to facilitate vesicle fusion and regulate membrane dynamics. Additionally they interact with components of the cytoskeleton to ensure vesicle movement within cells influencing both signaling and homeostatic pathways.

Aberrations in RAB proteins often link to neurological disorders and cancer. Mutations or dysregulation of specific RAB proteins can disrupt vesicle transport and lead to pathologies such as Charcot-Marie-Tooth disease or melanoma. In cancer changes in RAB function may alter signaling pathways that control cell proliferation and metastasis. Moreover RAB proteins can have interactions with oncogenes or neuroprotective proteins influencing disease progression and offering potential targets for therapeutic interventions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Required for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication.

See full target information AGFG1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cellular and molecular medicine 23:7246-7260 PubMed31454154

2019

FSP1 promotes the biofunctions of adventitial fibroblast through the crosstalk among RAGE, JAK2/STAT3 and Wnt3a/β-catenin signalling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Caihua Fu,Ping Liu,Peilun Li,Wenhui Liu,Xianwei Huang,Yansheng Liang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB antibody (AB244307)

Immunocytochemistry/ Immunofluorescence - Anti-RAB antibody (AB244307)

Western blot - Anti-RAB antibody (AB244307)

Western blot - Anti-RAB antibody (AB244307)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise