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AB243293

Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] - BSA and Azide free

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Rabbit Recombinant Monoclonal RAB10 phospho T73 antibody. Carrier free. Suitable for Dot, WB, ICC/IF and reacts with Synthetic peptide, Mouse, Human samples.
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Immunocytochemistry/ Immunofluorescence - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] - BSA and Azide free (AB243293)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] - BSA and Azide free (AB243293)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Saponin permeabilized MEF (mouse embryonic fibroblast) generated from wild-type or mutant LRRK2[R1441C] embryos without and with (+) MLi-2 treatment (100nM for 60 min) cells labeling RAB10 with ab241060 at 0.5 µg/mL, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)  secondary antibody at 1/1000 dilution (red). Nuclear counterstain is DAPI.

The image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241060).

Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] - BSA and Azide free (AB243293)
  • Dot

Supplier Data

Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] - BSA and Azide free (AB243293)

Dot Blot - Anti-RAB10 antibody [MJF-R21-22-5] (ab241060) at a 1/1000 dilution.

Lane 1 : Rab10 (phospho T73) peptide

Lane 2 : Rab10 non-phospho peptide

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100000 dilution.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 180 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241060).

관련 conjugated 항체와 다양한 조성의 항체 (3)

  • Unconjugated

    Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5]

  • Mouse version

    Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] - Mouse IgG2a (Chimeric)

  • Mouse version

    Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] - Mouse IgG2a (Chimeric) - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

MJF-R21-22-5

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

Dot, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB181367

Anti-RAB10 antibody [EPR13242]

20ul selling size|KO Validated|RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-RAB10 antibody [EPR13242] (AB181367)

  • 0
  • 0
Primary Antibodies

AB231707

Anti-RAB10 (phospho T73) antibody [MJF-R21] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] - BSA and Azide free (AB231707)

  • 0
  • 0
Primary Antibodies

AB238655

Anti-RAB10 antibody [MJF-R23] - BSA and Azide free

KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RAB10 antibody [MJF-R23] - BSA and Azide free (AB238655)

  • 0
  • 0
Primary Antibodies

AB230261

Anti-RAB10 (phospho T73) antibody [MJF-R21]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261)

  • 0
  • 0
Primary Antibodies

AB237703

Anti-RAB10 antibody [MJF-R23]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RAB10 antibody [MJF-R23] (AB237703)

  • 0
  • 0
Primary Antibodies

AB224886

Anti-RAB10 antibody [EPR13242] - BSA and Azide free

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-RAB10 antibody [EPR13242] - BSA and Azide free (AB224886)

  • 0
  • 0

보완 제품

Sample Prep & Detection Kits

AB102890

HRP Conjugation Kit - Lightning-Link®

Conjugation - HRP Conjugation Kit - Lightning-Link® (AB102890)

  • 4
  • 3
Sample Prep & Detection Kits

AB102855

APC/Cy5.5® Conjugation Kit - Lightning-Link®

Schematic Diagram - APC/Cy5.5® Conjugation Kit - Lightning-Link® (AB102855)

  • 0
  • 0
Sample Prep & Detection Kits

AB218260

Oligonucleotide Conjugation Kit

Conjugation - Oligonucleotide Conjugation Kit (AB218260)

  • 0
  • 0
Primary Antibodies

AB237703

Anti-RAB10 antibody [MJF-R23]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RAB10 antibody [MJF-R23] (AB237703)

  • 0
  • 0
Primary Antibodies

AB256487

Anti-RAB12 (phospho S106) antibody [MJF-R25-9]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (AB256487)

  • 0
  • 0
Primary Antibodies

AB230260

Anti-RAB8A (phospho T72) antibody [MJF-R20]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] (AB230260)

  • 4
  • 3
Primary Antibodies

AB133474

Anti-LRRK2 antibody [MJFF2 (c41-2)]

20ul selling size|KO Validated|RabMAb|Recombinant

Western blot - Anti-LRRK2 antibody [MJFF2 (c41-2)] (AB133474)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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제품 세부 정보

ab243293 is the carrier-free version of ab241060.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RAB10 also known as RAB10 member RAS oncogene family is a small GTPase belonging to the RAB protein family. It has a molecular weight of approximately 24 kDa. RAB10 is ubiquitously expressed with higher levels observed in brain liver and lung tissues. It cycles between an active GTP-bound state and an inactive GDP-bound state regulating various membrane trafficking events within the cell. Its role extends to modulating the transport of proteins from the Golgi apparatus to the plasma membrane therefore influencing cellular logistics and function.
Biological function summary

RAB10 participates in vesicular trafficking and transport processes. It is part of the endosomal and exocytic complexes contributing to the sorting and delivery of cellular cargo. RAB10's activity affects the delivery of glucose transporter type 4 (GLUT4) to the plasma membrane impacting glucose uptake and metabolism. It also plays a critical role in the polarized transport of cargo in neuronal cells influencing neuronal signaling and function.

Pathways

RAB10 integrates into the insulin signaling and polarized epithelial cell transport pathways. Within these RAB10 cooperates with proteins like GLUT4 and the TBC1D4 a RAB GAP protein essential for GLUT4 vesicle translocation. These interactions ensure efficient response and coordination of cellular trafficking required for metabolic regulation and cellular growth processes.

RAB10 shows connections to metabolic disorders and neurodegenerative diseases. Its role in glucose transporter trafficking links it to type 2 diabetes as improper GLUT4 trafficking disrupts glucose homeostasis. In the realm of neurodegenerative diseases proteins involved in neuronal transport and function such as APP and Tau may be influenced by RAB10-mediated pathways impacting diseases like Alzheimer's disease. The ability of RAB10 to interact with numerous cellular partners places it as a significant contributor to cellular dysfunction observed in these conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 21248164). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 21248164). RAB10 is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane (PubMed : 21248164). Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane (By similarity). In parallel, RAB10 regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response (By similarity). Also plays a specific role in asymmetric protein transport to the plasma membrane (PubMed : 16641372). In neurons, involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane (By similarity). In epithelial cells, regulates transport from the Golgi to the basolateral membrane (PubMed : 16641372). May play a role in the basolateral recycling pathway and in phagosome maturation (By similarity). May play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion (PubMed : 23263280). Together with LRRK2, RAB8A, and RILPL1, regulates ciliogenesis (PubMed : 30398148). When phosphorylated by LRRK2 on Thr-73, binds RILPL1 and inhibits ciliogenesis (PubMed : 30398148). Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-dependent endososomal export route (PubMed : 32344433). Targeted to and stabilized on stressed lysosomes through LRRK2 phosphorylation where it promotes the extracellular release of lysosomal content through EHBP1 and EHNP1L1 effector proteins (PubMed : 30209220).. (Microbial infection) Upon Legionella pneumophila infection promotes endoplasmic reticulum recruitment and bacterial replication. Plays a role in remodeling the Legionella-containing vacuole (LCV) into an endoplasmic reticulum-like vacuole.
See full target information RAB10 pT73

대체 명칭 보기

Ras-related protein Rab-10, RAB10
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Product promise

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