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AB325799

Anti-RAB12 antibody [MJF-D28814-65R]

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Rabbit Recombinant Monoclonal RAB12 antibody. Suitable for WB and reacts with Human, Mouse samples.
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Western blot - Anti-RAB12 antibody [MJF-D28814-65R] (AB325799)
  • WB

Lab

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] (AB325799)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] (ab325799) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

RAB12 knockout U-87 MG whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 27 kDa

false

Exposure time: 180s

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] (AB325799)
  • WB

Lab

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] (AB325799)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The data was kindly provided by a collaborator.

The identity of the bands higher than 45kda are unknown.

In Western blot, Anti-alpha Tubulin antibody - Loading Control (37KDa).

All lanes:

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] (ab325799) at 1/1000 dilution

Lane 1:

Wild-type A549 (human lung carcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

RAB12 knockout A549 whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 27 kDa,37 kDa

false

Exposure time: 180s

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] (AB325799)
  • WB

Lab

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] (AB325799)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver (PMID : 8575079, PMID : 1313420).

Samples are non-boiled as boiling may cause protein aggregation.

The identity of the bands higher than 45kda are unknown.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) 1 : 100000 (15Kda).

All lanes:

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] (ab325799) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 50 µg

Lane 2:

Mouse liver tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 27 kDa,15 kDa

false

Exposure time: 3s

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] (AB325799)
  • WB

Lab

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] (AB325799)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The data was kindly provided by a collaborator.

The identity of the bands higher than 45kda are unknown.

In Western blot, Anti-alpha Tubulin antibody - Loading Control (37KDa).

All lanes:

Western blot - Anti-RAB12 antibody [MJF-D28814-65R] (ab325799) at 1/1000 dilution

Lane 1:

Wild-type MEF (mouse embryo fibroblast) whole cell lysate at 10 µg

Lane 2:

RAB12 knockout MEF whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 27 kDa,37 kDa

false

Exposure time: 180s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

MJF-D28814-65R

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB230261

Anti-RAB10 (phospho T73) antibody [MJF-R21]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261)

  • 0
  • 0
Primary Antibodies

AB230260

Anti-RAB8A (phospho T72) antibody [MJF-R20]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] (AB230260)

  • 4
  • 3
Primary Antibodies

AB133450

Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (AB133450)

  • 5
  • 2
Primary Antibodies

AB237703

Anti-RAB10 antibody [MJF-R23]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RAB10 antibody [MJF-R23] (AB237703)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy (By similarity).
See full target information RAB12

대체 명칭 보기

Ras-related protein Rab-12, RAB12
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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