Anti-RAB45 antibody [EPR16349] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (AB251195)

This data was developed using ab194827, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of K562 cells labeling RAB45 using ab194827 at 1/50 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody : Blue. Rabbit monoclonal IgG was used as isotype control (Black).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (AB251195)

This data was developed using ab194827, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells labeling RAB45 with ab194827 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain : DAPI. Cytoplasm staining on HeLa cell line was observed.

• WB

Supplier Data

Western blot - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (AB251195)

This data was developed using ab194827, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RAB45 antibody [EPR16349] (<a href='/ko/products/primary-antibodies/rab45-antibody-epr16349-ab194827'>ab194827</a>) at 1/10000 dilution

All lanes:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 83 kDa

false

• WB

Supplier Data

Western blot - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (AB251195)

This data was developed using ab194827, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RAB45 antibody [EPR16349] (<a href='/ko/products/primary-antibodies/rab45-antibody-epr16349-ab194827'>ab194827</a>) at 1/1000 dilution

Lane 1:

A549 cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

Human testis lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa,83 kDa

false

• WB

Supplier Data

Western blot - Anti-RAB45 antibody [EPR16349] - BSA and Azide free (AB251195)

This data was developed using ab194827, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RAB45 antibody [EPR16349] (<a href='/ko/products/primary-antibodies/rab45-antibody-epr16349-ab194827'>ab194827</a>) at 1/10000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Lane 5:

Rat heart lysate at 10 µg

Lane 6:

C6 cell lysate at 10 µg

Lane 7:

RAW 264.7 cell lysate at 10 µg

Lane 8:

PC-12 cell lysate at 10 µg

Lane 9:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 83 kDa

false