AB240974

Anti-RAB7B antibody [EPR15727] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal RAB7B antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples.

대체 명칭 보기

Ras-related protein Rab-7b, RAB7B

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] - BSA and Azide free (AB240974)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] - BSA and Azide free (AB240974)

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling RAB7B with ab193360 at 1/150 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193360)

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-RAB7B antibody [EPR15727] - BSA and Azide free (AB240974)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RAB7B antibody [EPR15727] - BSA and Azide free (AB240974)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed U937 cells labeling RAB7B with ab193360 at 1/270 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193360)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] - BSA and Azide free (AB240974)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling RAB7B with ab193360 at 1/150 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193360)

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15727

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab240974 is the carrier-free version of ab193360.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RAB7B also known as Ras-related protein Rab-7b is a small GTPase belonging to the Ras superfamily. This protein weighs approximately 23 kDa. Researchers find RAB7B in various tissues including the immune and the central nervous system. It plays a direct role in intracellular protein trafficking and vesicular transport moving proteins and other molecules from one organelle to another within cells. Through its association with specific membrane compartments RAB7B assists in maintaining the dynamic balance of vesicle budding transport and fusion regulating endosomal sorting.

Biological function summary

The functions of RAB7B include its contribution to lysosomal biogenesis and endosome-to-lysosome trafficking. It is part of a complex known as the endosomal transport modulation machinery where it interacts with multiple effector proteins to translocate cargo to the lysosome for degradation or recycling. In immune cells RAB7B helps modulate immune response by controlling the degradation of signaling receptors. This function makes it essential for cellular homeostasis and adaptive immune responses influencing antigen processing.

Pathways

The interaction of RAB7B with other members of the Rab GTPase family situates it in important cellular pathways like the endocytic pathway and autophagy. In the endocytic pathway it coordinates with proteins like RAB5A transitioning early endosomes to late endosomes. The action in autophagy involves interaction with other autophagic regulators to aid in autophagosomal-lysosomal fusion influencing how cells handle stress and remove damaged components.

Abnormal RAB7B expression has been linked to neurodegenerative diseases like Alzheimer's and immune disorders such as rheumatoid arthritis. Its interaction with other proteins like LRRK2 in Parkinson's disease implicates it in pathways that lead to neuronal damage and inflammation. In rheumatoid arthritis RAB7B influences immune cell signaling and cytokine release contributing to autoimmune responses. Understanding its associations aids in developing therapeutic strategies targeting the molecular functions of RAB7B in these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Controls vesicular trafficking from endosomes to the trans-Golgi network (TGN). Acts as a negative regulator of TLR9 signaling and can suppress TLR9-triggered TNFA, IL6, and IFNB production in macrophages by promoting TLR9 lysosomal degradation. Also negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Promotes megakaryocytic differentiation by increasing NF-kappa-B-dependent IL6 production and subsequently enhancing the association of STAT3 with GATA1. Not involved in the regulation of the EGF- and EGFR degradation pathway.

See full target information Ras-related protein Rab-7b

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RAB7B antibody [EPR15727] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] - BSA and Azide free (AB240974)

Flow Cytometry (Intracellular) - Anti-RAB7B antibody [EPR15727] - BSA and Azide free (AB240974)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] - BSA and Azide free (AB240974)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise