AB172730

Rabbit IgG, monoclonal [EPR25A] - Isotype Control

KD Validated
RabMAb
Advanced Validation
Recombinant
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Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) is a rabbit monoclonal antibody used in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF, ChIP-seq, ChIC/CUT&RUN-seq.

- Biophysical QC for unrivalled batch-batch consistency
- Over 610 publications

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cyt

Lab

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Overlay histogram showing A549 (human lung carcinoma) cells stained with ab133557 (red line). The cells were fixed with 2% paraformaldehyde. The cells were then incubated with ab133557 at 1/60 dilution. The secondary antibody used was goat anti-rabbit IgG (FITC) at 1/150 dilution. Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 530/30 bandpass filter.

Conjugated versions are available for this clone : Alexa Fluor^® 488 (ab199091), Alexa Fluor^® 647 (ab199093), R-PE (ab209478), APC (ab232814).

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Raji (human Burkitt's lymphoma B lymphocyte, Right) BxPC-3 (human pancreas adenocarcinoma epithelial cell, Left) cells labelling with ab327424 at 1/50 dilution (1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Low expression : BxPC-3.

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometry overlay histogram of 4% Paraformaldehyde fixed 90% methanol permeabilized HaCaT (human skin keratinocyte) (Left) HeLa (human cervix adenocarcinoma epithelial cell) (Right) cells labelling Involucrin with ab181980 at 1/1000 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081), at 1/2000 dilution was used as the secondary antibody.

Negative sample : HeLa.

Flow Cyt

Supplier Data

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling IL-10RA with ab323700 at 1/500 dilution (0.1 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

human PBMC are co-stained with CD14 conjugated Alexa Fluor®647.
Gated on viable cell.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric overlay histogram of 4% paraformaldehyde-fixed, 90% methanol permeabilised HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labelling REG3A + REG3G with Anti-REG3A + REG3G antibody (ab202057) at 1 µg/test (1/50 dilution) (red line). Isotype control antibody (black line) was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody. A Goat Anti-Rabbit IgG Alexa Fluor^® 488 secondary antibody (ab150081) was used at 1/5000 dilution. Unlabelled sample (blue line), consisting of cells without incubation with either the primary or secondary antibody, was also used as a control.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized BT-549 (human Breast Mammary gland epithelial) treated with 300ng/ml BFA for 20 hours (Green and black) and Untreated control (Magenta and grey) cells labelling with ab326942 at 1/5000 dilution (0.01ug) / Magenta and Green compared with a Rabbit monoclonal IgG (ab172730) / Black and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T cells transfected with a human IL12B expression vector containing a HA-Myc-tag® (Upper) 293T cells transfected with a human IL12A expression vector containing a HA-Myc-tag® (Lower left) 293T cells transfected with an empty expression vector containing a myc-His-tag® (Lower right) cells labelling IL12 p40 with ab327081 at 1/50000 dilution (0.001ug) / Upper right and Lower compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt

Supplier Data

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

human PBMC are co-stained with CD56 conjugated BV421.
Gated on viable lymphocyte.

Flow Cyt

Supplier Data

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

human PBMC are co-stained with CD4 conjugated BV421.
Gated on viable lymphocyte.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometry overlay histogram showing left Ramos positive cells and right negative HEK293 stained with ab78237 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab78237) (1x 106 in 100μl at 0.2μg/ml (1/2500)) for 30min at 22°C.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C

Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

This antibody gave a positive signal in Ramos Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) labelling BRD8/p120 with ab325801 at 1/500 dilution (0.1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CLEC1B with ab327705 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were co-stained with CD3 conjugated to Alexa Fluor®647.

Flow Cyt

Supplier Data

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Raji (human Burkitt's lymphoma B lymphocyte) (Right) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood) (Left) cells labelling CD19 with ab322345 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Flow Cyt

Lab

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were co-stained with CD14 conjugated to PE.
PMID : 10671229

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte) (Right) HepG2 (human hepatocellular carcinoma epithelial cell) (Left) cells labelling RIP3 with ab325636 at 1/500 dilution (0.1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Negative control : HepG2.

Flow Cyt

Supplier Data

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD19 with ab322345 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD20 conjugated to Alexa Fluor®647.
Gated on viable cells.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T cells transfected with a human PLCZ1 expression vector containing a myc-His-tag® (Middle) 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling PLCZ1 with ab325528 at 1/5000 dilution (0.01ug) / Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling CPS1 (red) with ab129076 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

Flow Cyt

Supplier Data

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CXCL16 with ab322709 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Human PBMC were co-stained with CD19 conjugated Alexa Fluor®647.
Gated on viable cell.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T cells transfected with a human IFNL3 expression vector containing a HA-Myc-tag® (Upper) 293T cells transfected with a human IFNL2 expression vector containing a HA-Myc-tag® (Lower left) 293T cells transfected with an empty expression vector containing a myc-His-tag® (Lower right) cells labelling with ab327709 at 1/5000 dilution (0.01ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (human embryonic kidney epithelial cell) cells transfected with a human LPL expression vector containing a myc-His-tag® (middle) 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling with ab328031 at 1/5000 dilution (0.01ug) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized
293T cells transfected with a human NPM (mutation) expression vector containing a HA-Myc-tag® (Upper)
293T cells transfected with a human NPM (WT) expression vector containing a HA-Myc-tag® (Lower left)
293T cells transfected with an empty expression vector containing a myc-His-tag® (Lower right)
labelling NPM1-mutated with ab326567 at 1/5000 dilution (0.01ug) / Upper right and Lower left/right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) cells labelling MAVS with ab290729 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Human PBMC (human peripheral blood mononuclear cell) were treated with cell stimulation cocktail for 6h (Lower left and lower right) / Untreated human PBMC (Upper left and upper right) cells labelling IL-2 with ab323413 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD3 conjugated to Alexa Fluor®647.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD19 conjugated to PE/Cy7.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a human IL-2 expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling IL-2 with ab323413 at 1/5000 dilution (0.01ug)/ Middle and right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti Myc conjugated to Alexa Fluor® 647.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Untreated human PBMC (Human peripheral blood mononuclear cell) (upper left and upper right) / Human PBMC treated with cell stimulation cocktail for 6 hours (down left and down right) cells labelling IL17A with ab323803 at 1/500 dilution (0.1 ug) / upper right and down right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Human PBMC were co-stained with anti human CD4 conjugated to BV421.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Human PBMC were co-stained with anti human CD19 conjugated to Alexa Fluor®647.

Flow Cyt

Supplier Data

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / 293T (human embryonic kidney epithelial cell) cells transfected with a human IL17A expression vector containing a Myc tag (Middle) / 293T cells transfected with an empty expression vector containing a Myc tag (Right) cells labelling IL17A with ab323803 at 1/5000 dilution (0.01 ug) / Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of SNU-16 (human Stomach epithelial, Right) A549 (human lung carcinoma epithelial cell, Left) cells labelling BTNL3 with ab327704 at 1/50 dilution (1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Low expression : A549.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HUVEC (human umbilical vein endothelial cell) treated with 0.5 ug/ml LPS for 24 hours and with 300ng/ml Brefeldin A for the last 20 hours (Green and black) and Untreated HUVEC (Magenta and grey) cells labelling with ab326942 at 1/500 dilution (0.1ug) / Magenta and Green compared with a Rabbit monoclonal IgG (ab172730) / Black and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma epithelial cell) labelling Rad51 with purified ab133534 at 1/200 (red). Cells were fixed with 4% paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488 ab150077 (1/2000) was used as the secondary antibody. Black - Isotype control, (ab172730) rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell, Left) / A-204 (human muscle rhabdomyosarcoma cell, Right) cells labelling NFATC4 with ab324108 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Low expression : HepG2.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HaCaT (human skin keratinocyte, Left) / HeLa (human cervical adenocarcinoma epithelial cell, Right) cells labelling NFATC4 with ab324108 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Low expression : HaCaT (PMID : 27893713).

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) labelling c-Myc with ab32072 at 1/50 (red). Cells were fixed with 4% paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488 ab150077 (1/2000) was used as the secondary antibody. Black - Isotype control, (ab172730) rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized 293T cells (human embryonic kidney epithelial cell) transfected with a human CLEC1B expression vector containing a Myc-His-tag® (Middle) 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling with ab327705 at 1/50000 dilution (0.001ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with isotype control or our antibody. Then fixed with 4% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.

Flow Cyt

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Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of Daudi (human Burkitt's lymphoma lymphoblast, Left) TF-1 (human erythroleukemia erythroblast, Right) cells labelling Leptin Receptor with ab325970 at 1/50 dilution (1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Low expression : Daudi.

Flow Cyt

Supplier Data

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD3 conjugated to Brilliant Violet 421.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Human PBMC were co-stained with CD11b conjugated BV421.
Gated on viable cell.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometry overlay histogram showing left HeLa positive cells and right negative MCF7 stained with ab108349 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody ab108349 (1/500) for 30min at 22°C.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human PBMC (human peripheral blood mononuclear cell) treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 6 days then add 10ng/ml TNF alpha(human), 1000U/ml IL-6(human), 10ng/ml IL-1 beta(human) and 1ug/ml PGE2 for 2 days (Lower left and right) Untreated Human PBMC (Upper left and right) cells labelling IL12 p40 with ab327081 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with anti-CD11c conjugated to Alexa Fluor®488. Then fixed with 4% PFA for 10 min followed by intracellularly staining with rabbit IgG or our antibody. Maturation of dendritic cells (DCs) is induced by treatment with 100 ng/mL GM-CSF and 50 ng/mL IL-4 for 6 days, followed by the addition of 10 ng/mL TNF-α (human), 1000 U/mL IL-6 (human), 10 ng/mL IL-1β (human), and 1 μg/mL PGE2 for an additional 2 days (PMID : 22882679).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25A

Isotype

IgG

Carrier free

Applications

ChIP-seq, IP, ChIC/CUT&RUN-seq, WB, Flow Cyt (Intra), IHC-P, Flow Cyt, ICC/IF

applications

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "0.5-2 µg", "ChICCUTRUNseq-species-notes": "", "ChIPseq-species-checked": "", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, ChIP-seq, Flow Cyt, ICC/IF, IHC-P, IP, and WB.

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) was first used in a scientific publication in 2014 and has been cited over 617 times in peer reviewed journals. It's performance in Western blot, immunofluorescence, IHC and ChIP in human, mouse and rat samples is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) is sold in 200 µg selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR25A - ab210849.

Antibody clone EPR25A is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor^® 488, Alexa Fluor^® 647, HRP, Alexa Fluor^® 45, Alexa Fluor^® 594, Alexa Fluor^® 555, PE, Alexa Fluor^® 568, PerCP, FITC, APC, Biotin, Alexa Fluor^® 75 (ab19991, ab19993, ab19957, ab2815, ab28568, ab28569, ab29478, ab29613, ab22217, ab223339, ab232814, ab3273, ab32286).

One of the top cited IgG Isotype control antibodies in the market with >1000 citations. IgG Isotype Control antibodies are essential for multiple applications. Isotype Control ab172730 is suitable for ChIC/CUT&RUN-seq, IP, ICC/IF, IHC-P,Flow Cyt, WB. This negative control antibodies help distinguish specific antibody binding from non-specific interactions, ensuring reliable results. Optimize your research with high-quality IgG Isotype Control antibodies for precise data interpretation

Immunogen
Chemical / Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH forms a large complex composed of ~50 kDa subunits.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

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타겟 정보

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제품이 사용된 논문 (1211)

Recent publications for all applications. Explore the 전체 목록and refine your search

Journal of inflammation (London, England) 22:41 PubMed41057834

2025

Baicalein inhibits mycobacterium tuberculosis-induced macrophage M1 polarization depending on the regulation of YY1/RAB10/TLR4 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Zhou,Shuanghua Chen,Linfei Shu,Qian Wang,Ting Yuan,Yangjing Ou,Ling Qing,Xiaojin He

Oncology research 33:3023-3040 PubMed41050080

2025

Competitive Sequestration of miR-1183 by lncRNA DDX11-AS1 Drives Gliomagenesis through E2F7 Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwei Wang,Xinzhi Yang,Lvbiao Lin,Jianbo Yu,Jie Mao

Journal of cell communication and signaling 19:e12032 PubMed41050263

2025

Emerging role of HIC1 in prostate cancer progression and therapeutic response: A novel perspective.

Applications

Unspecified application

Species

Unspecified reactive species

Dun Xue,Long Tan,Fengshuai Yang,Xiaolan Tian,Qian Zuo,Xinghui Wu

Acta pharmaceutica Sinica. B 15:4751-4771 PubMed41049757

2025

as a super-enhancer-regulated gene drives T-ALL progression HIF1A deubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Xu,Zimu Zhang,Juanjuan Yu,Tongting Ji,Jia Cheng,Xiaodong Fei,Xinran Chu,Yanfang Tao,Yan Xu,Pengju Yang,Wenyuan Liu,Gen Li,Yongping Zhang,Yan Li,Fenli Zhang,Ying Yang,Bi Zhou,Yumeng Wu,Zhongling Wei,Yanling Chen,Jianwei Wang,Di Wu,Xiaolu Li,Yang Yang,Guanghui Qian,Hongli Yin,Shuiyan Wu,Shuqi Zhang,Dan Liu,Jun-Jie Fan,Lei Shi,Xiaodong Wang,Shaoyan Hu,Jun Lu,Jian Pan

World journal of surgical oncology 23:350 PubMed41024019

2025

SUZ12 knockdown restrains the proliferation, migration, and invasion of oral tongue squamous cell carcinoma through inhibiting DNMT1-mediated ZNF582 promoter methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangwei Kong,Yicheng Cheng,Lin Zhang,Wei Yin,Chenchen Wang,Zhan'ao Wu

Hereditas 162:186 PubMed40999468

2025

The FOXD1/NAT10 positive feedback loop drives nasopharyngeal carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Leifeng Liu,Qizhu Chen,Yiling Li,Weihao Wu,Feng Jiang,Haitao Qiu

Journal of orthopaedic surgery and research 20:831 PubMed40993725

2025

USP28 participates in high glucose-mediated endothelial dysfunction via deubiquitinating SIRT1 protein in diabetic foot ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Liu,Jin Zhang,Jichang Bai,Kuanzhi Liu

eLife 13: PubMed40996791

2025

Steady-state neuron-predominant LINE-1 encoded ORF1p protein and LINE-1 RNA increase with aging in the mouse and human brain.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Bonnifet,Sandra Sinnassamy,Olivia Massiani-Beaudoin,Philippe Mailly,Heloise Monnet,Damarys Loew,Berangere Lombard,Nicolas Servant,Rajiv L Joshi,Julia Fuchs

JCI insight 10: PubMed40985894

2025

FXR shapes an immunosuppressive microenvironment in PD-L1lo/- non-small cell lung cancer by upregulating HVEM.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Xu,Bin Shang,Hancheng Wu,Xiuye Jin,Junren Wang,Jing Li,Daowei Li,Bin Liang,Xingguang Wang,Lili Su,Wenjie You,Shujuan Jiang

Journal of cell communication and signaling 19:e70046 PubMed40977851

2025

Tri-domain proteins 27 alleviates ischemia-reperfusion injury-induced acute kidney injury by promoting Gli-like transcription factor 1 expression via the inhibition of polycomb repressive complex 2 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Chongxiang Xiong,Haishan Chen,Baoting Su,Li Zhang,Jingxiang Hu,Qiaowen Wang,Shougang Zhuang

제품이 사용된 논문 모두 보기

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chicCutRunSequencingBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rabbit IgG, monoclonal [EPR25A] - Isotype Control

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

Flow Cytometry (Intracellular) - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

관련 conjugated 항체와 다양한 조성의 항체 (15)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Reagents, Controls & Accessories

Sample Prep & Detection Kits

Secondary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건