AB254907

Anti-RABEP2 antibody

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Rabbit Polyclonal RABEP2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RABEP2 aa 150 to C-terminus.

대체 명칭 보기

RABPT5B, RABEP2, Rab GTPase-binding effector protein 2, Rabaptin-5beta

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-RABEP2 antibody (AB254907)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RABEP2 antibody (AB254907)

PFA fixed, Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling RABEP2 using ab254907 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABEP2 antibody (AB254907)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABEP2 antibody (AB254907)

Paraffin-embedded human urinary bladder tissue stained for RABEP2 using ab254907 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-RABEP2 antibody (AB254907)

Lane 1:

Western blot - Anti-RABEP2 antibody (ab254907)

Lane 2:

Western blot - Anti-RABEP2 antibody (ab254907) at 0.4 µg/mL

Lane 1:

DNA Ladder

Lane 2:

RT4 cell lysate

Predicted band size: 64 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RABEP2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H5N1

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RABEP2 also known as Rabaptin-5-like protein or Rabaptin-5b is a protein involved in endosomal trafficking. It has a molecular weight of approximately 50 kDa. RABEP2 functions as an effector protein for Rab GTPases which are critical regulators of membrane traffic in the cell. This protein is widely expressed in various tissues playing key roles in intracellular transport processes. RABEP2 associates with particular endosomal membranes facilitating vesicle budding movement and fusion.

Biological function summary

RABEP2 facilitates membrane trafficking by working in the context of endosomal membrane fusion. It forms part of a larger protein complex that interacts with Rab GTPases especially Rab5 which is a well-known regulator of early endosome dynamics. By binding these GTPases RABEP2 stabilizes active Rab GTPase configurations assisting in their recruitment to endosomal membranes. This mechanism is essential for efficient transport and sorting of internalized molecules.

Pathways

RABEP2 plays a role in cellular pathways involving receptor-mediated endocytosis and vesicle trafficking. In these pathways it connects with the early endosome and sorting endosome pathways which are vital for recycling and degradation processes. RABEP2 works closely with other Rab family proteins like Rab4 and Rab11 sharing roles in ensuring proper receptor allocation and signal transduction. This cooperation enhances the cell's ability to adaptively respond to changes in the extracellular environment.

Modifications or dysfunctions in RABEP2 have associations with cancer and neurodegenerative diseases. Disruptions in Rab GTPase networks including alterations involving RABEP2 can lead to dysfunctional endosomal trafficking which contributes to tumor progression by affecting growth factor receptor recycling. Relationships exist between RABEP2 functionality and other proteins like Rab5 where altered interactions may result in neurodegenerative conditions due to the cumulative effects on synaptic function and neuronal survival.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a role in membrane trafficking and in homotypic early endosome fusion (PubMed : 9524116). Participates in arteriogenesis by regulating vascular endothelial growth factor receptor 2/VEGFR2 cell surface expression and endosomal trafficking (PubMed : 29425100). By interacting with SDCCAG8, localizes to centrosomes and plays a critical role in ciliogenesis (PubMed : 27224062).

See full target information RABEP2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology letters 22:532 PubMed34079591

2021

miR-590-5p targets RMND5A and promotes migration in pancreatic adenocarcinoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Sixing Chen,Yu Chen,Yao Wen,Wanwan Cai,Ping Zhu,Wuzhou Yuan,Yongqing Li,Xiongwei Fan,Yongqi Wan,Fang Li,Jian Zhuang,Zhigang Jiang,Xiushan Wu,Yuequn Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RABEP2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RABEP2 antibody (AB254907)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABEP2 antibody (AB254907)

Western blot - Anti-RABEP2 antibody (AB254907)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise