AB196024

Anti-RABL3 antibody [EPR16709] - C-terminal

RabMAb
Recombinant
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal RABL3 antibody. C-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Rat samples. Cited in 2 publications.

대체 명칭 보기

Rab-like protein 3, RABL3

6 이미지

Immunocytochemistry/ Immunofluorescence - Anti-RABL3 antibody [EPR16709] - C-terminal (AB196024)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RABL3 antibody [EPR16709] - C-terminal (AB196024)

Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labeling RABL3 with ab196024 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/500. In blue DAPI staining.

Flow Cytometry (Intracellular) - Anti-RABL3 antibody [EPR16709] - C-terminal (AB196024)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RABL3 antibody [EPR16709] - C-terminal (AB196024)

Intracellular Flow Cytometry analysis of paraformaldehyde-fixed MCF7 cells labeling RABL3 with ab196024 at a 1/100 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, isotype control rabbit IgG (black) and unlabeled control cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL3 antibody [EPR16709] - C-terminal (AB196024)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL3 antibody [EPR16709] - C-terminal (AB196024)

Immunohistochemical analysis of paraffin-embedded, human renal adenocarcinoma tissue labeling RABL3 with ab196024 at a 1/100 dilution. Counter stained with hematoxylin. As secondary antibody goat anti-rabbit IgG H&L (HRP) ab97051 was used at a 1/500. In blue DAPI staining.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL3 antibody [EPR16709] - C-terminal (AB196024)

Immunohistochemical analysis of paraffin-embedded, rat liver tissue labeling RABL3 with ab196024 at a 1/100 dilution. Counter stained with hematoxylin. As secondary antibody goat anti-rabbit IgG H&L (HRP) ab97051 was used at a 1/500. In blue DAPI staining.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-RABL3 antibody [EPR16709] - C-terminal (AB196024)

All lanes:

Western blot - Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024) at 1/1000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

true

Supplier Data

Western blot - Anti-RABL3 antibody [EPR16709] - C-terminal (AB196024)

All lanes:

Western blot - Anti-RABL3 antibody [EPR16709] - C-terminal (ab196024) at 1/10000 dilution

All lanes:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

true

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16709

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RABL3 also known as Rab-like protein 3 is a member of the Rab-like small GTPase family. It has a molecular mass of approximately 28 kDa. RABL3 plays a central role in cellular trafficking and transport by acting as a molecular switch cycling between an active GTP-bound state and an inactive GDP-bound state. Expression of RABL3 is widespread across various tissues although its exact expression pattern may vary depending on the cell type and physiological condition.

Biological function summary

RABL3 functions in the regulation of intracellular signaling pathways. It associates with other molecular partners to influence the dynamics of cargo transport through vesicles. While it is not specifically identified as part of a larger complex its interactions with other small GTPases suggest a collaborative role in vesicle docking and fusion processes. RABL3's function impacts cell communication and signal transduction vital processes in maintaining cellular homeostasis and response to environmental signals.

Pathways

RABL3 engages in the RAS signaling pathway where it may regulate downstream effects of RAS activation. In particular it can interact with proteins like RAB6 and RABL2 which share similar roles in vesicular trafficking and transport. The RAS signaling pathway's influence in growth and metabolism provides insight into RABL3's potential to modulate signal transduction efficiency and cellular response mechanisms.

Defects in RABL3 are associated with certain forms of cancer and neurodegenerative disorders. Its involvement is particularly noted in its connection to the KRAS protein which is significantly implicated in oncogenic transformations. Alterations in RABL3 expression or function may disrupt normal cellular signaling and transport contributing to disease progression. Understanding these interactions can help target therapeutic strategies for conditions related to these dysregulated pathways.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Required for KRAS signaling regulation and modulation of cell proliferation (PubMed : 31406347). Regulator of KRAS prenylation, and probably prenylation of other small GTPases (PubMed : 31406347). Required for lymphocyte development and function (By similarity). Not required for myeloid cell development (By similarity).

See full target information Rab-like protein 3

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

EMBO molecular medicine 15:e17376 PubMed37534622

2023

Viral anti-inflammatory serpin reduces immuno-coagulopathic pathology in SARS-CoV-2 mouse models of infection.

Applications

Unspecified application

Species

Unspecified reactive species

Liqiang Zhang,Yize Henry Li,Karen Kibler,Simona Kraberger,Arvind Varsani,Julie Turk,Nora Elmadbouly,Emily Aliskevich,Laurel Spaccarelli,Bereket Estifanos,Junior Enow,Isabela Rivabem Zanetti,Nicholas Saldevar,Efrem Lim,Jessika Schlievert,Kyle Browder,Anjali Wilson,Fernando Arcos Juan,Aubrey Pinteric,Aman Garg,Henna Monder,Rohan Saju,Savanah Gisriel,Bertram Jacobs,Timothy L Karr,Esther Borges Florsheim,Vivek Kumar,John Wallen,Masmudur Rahman,Grant McFadden,Brenda G Hogue,Alexandra R Lucas

Journal of cell science 135: PubMed36052645

2022

The atypical small GTPase RABL3 interacts with RAB11 to regulate early ciliogenesis in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tetsuo Kobayashi,Tatsuya Ikeda,Reo Ota,Takafumi Yasukawa,Hiroshi Itoh

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RABL3 antibody [EPR16709] - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-RABL3 antibody [EPR16709] - C-terminal (AB196024)

Flow Cytometry (Intracellular) - Anti-RABL3 antibody [EPR16709] - C-terminal (AB196024)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL3 antibody [EPR16709] - C-terminal (AB196024)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RABL3 antibody [EPR16709] - C-terminal (AB196024)

Western blot - Anti-RABL3 antibody [EPR16709] - C-terminal (AB196024)

Western blot - Anti-RABL3 antibody [EPR16709] - C-terminal (AB196024)

관련 conjugated 항체와 다양한 조성의 항체 (10)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise