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AB243905

Anti-Rad21 antibody [BLR052F]

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Rabbit Recombinant Monoclonal RAD21 antibody. Suitable for WB, Flow Cyt and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human RAD21 aa 550 to C-terminus.

대체 명칭 보기

HR21, KIAA0078, NXP1, SCC1, RAD21, Double-strand-break repair protein rad21 homolog, hHR21, Nuclear matrix protein 1, SCC1 homolog, NXP-1

3 이미지
Flow Cytometry - Anti-Rad21 antibody [BLR052F] (AB243905)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Rad21 antibody [BLR052F] (AB243905)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human Rad21 with ab243905 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)

Western blot - Anti-Rad21 antibody [BLR052F] (AB243905)
  • WB

Unknown

Western blot - Anti-Rad21 antibody [BLR052F] (AB243905)

Western blot analysis using ab243905 at 1/1000 dilution.

Lane 1 : HeLa whole cell lysate (50 µg).

Lane 2 : Jurkat whole cell lysate (50 µg).

Lane 3 : HEK-293T whole cell lysate (50 µg).

Lane 4 : RKO whole cell lysate (50 µg).

Lane 5 : GaMG whole cell lysate (50 µg).

Lane 6 : MCF7 whole cell lysate (50 µg).

Lane 7 : A549 whole cell lysate (50 µg).

Lane 8 : U-2 OS whole cell lysate (50 µg).

A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection : chemiluminescence with an exposure time of 3 seconds.

All lanes:

Western blot - Anti-Rad21 antibody [BLR052F] (ab243905)

Predicted band size: 72 kDa

false

Western blot - Anti-Rad21 antibody [BLR052F] (AB243905)
  • WB

Unknown

Western blot - Anti-Rad21 antibody [BLR052F] (AB243905)

Western blot analysis using ab243905 at 1/1000 dilution.

Lane 1 : NIH/3T3 whole cell lysate (50 µg).

Lane 2 : TCMK-1 whole cell lysate (50 µg).

A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection : chemiluminescence with an exposure time of 3 seconds.

All lanes:

Western blot - Anti-Rad21 antibody [BLR052F] (ab243905)

Predicted band size: 72 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR052F

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human RAD21 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60216

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대체품

Primary Antibodies

AB992

Anti-Rad21 antibody

Immunoprecipitation - Anti-Rad21 antibody (AB992)

  • 4
  • 21
Primary Antibodies

AB175215

Anti-Rad21 antibody [EPR12647]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Rad21 antibody [EPR12647] (AB175215)

  • 0
  • 0
Primary Antibodies

AB241395

Anti-Rad21 antibody [EPR12647] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Rad21 antibody [EPR12647] - BSA and Azide free (AB241395)

  • 0
  • 0
Primary Antibodies

AB154769

Anti-Rad21 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody (AB154769)

  • 0
  • 0
Primary Antibodies

AB217678

Anti-Rad21 antibody [EPR22506-15] - ChIP Grade

20ul selling size|BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody [EPR22506-15] - ChIP Grade (AB217678)

  • 4
  • 2
Primary Antibodies

AB254483

Anti-Rad21 antibody [EPR22506-15] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody [EPR22506-15] - BSA and Azide free (AB254483)

  • 0
  • 0

보완 제품

Primary Antibodies

AB9263

Anti-SMC3 antibody

Immunoprecipitation - Anti-SMC3 antibody (AB9263)

  • 5
  • 7
Primary Antibodies

AB4729

Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729)

  • 5
  • 85
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB8580

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

  • 5
  • 90
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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제품 세부 정보

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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특성 및 보관 정보

제형
Liquid
Purification 관련 사항
Recombinant antibody was purified from cell culture supernatant.
보관 버퍼
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Rad21 also known as double-strand-break repair protein rad21 homolog serves as a component of the cohesin complex. This protein is approximately 123 kDa in size. Rad21 is expressed in a wide range of tissues and cell types particularly in those with high proliferative activity. The protein plays an essential mechanical role by facilitating the cohesion of sister chromatids during cell division ensuring successful chromosome segregation.
Biological function summary

Rad21 is essential in processes beyond chromatid cohesion. It forms part of the cohesin complex which also includes proteins like SMC1 SMC3 and SCC3/SA1 or SA2. This complex is central to maintaining genome stability by participating in DNA repair and regulating gene expression. Through these roles Rad21 contributes to cellular processes such as cell cycle progression and embryonic development.

Pathways

Rad21 integrates into the DNA damage response and cell cycle regulation pathways. The cohesin complex with Rad21 as an important factor acts alongside other proteins such as ATM and ATR in the DNA damage response ensuring the repair of double-strand breaks. Within the cell cycle Rad21 helps coordinate the timing and accuracy of cell division working with cyclin-dependent kinases.

Rad21 has been linked to cancer and Cornelia de Lange syndrome. Mutations or dysregulation of Rad21 can result in tumorigenesis due to errors in chromosome segregation and compromised DNA repair. In Cornelia de Lange syndrome cohesin-related proteins like NIPBL and SMC1A also play a role indicating a disruption in the cohesin pathway's function. Understanding Rad21's involvement helps in developing targeted therapies for these conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Double-strand-break repair protein rad21 homolog. As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions (PubMed : 11509732). The cohesin complex may also play a role in spindle pole assembly during mitosis (PubMed : 11590136). In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (By similarity). May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter (PubMed : 25575569). May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity).. 64-kDa C-terminal product. May promote apoptosis.
See full target information RAD21
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Product promise

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