Anti-Rad51D antibody [EPR16205] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] - BSA and Azide free (AB236144)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human lung carcinoma tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202063).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] - BSA and Azide free (AB236144)

Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Nuclear staining on Human small intestine tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202063).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] - BSA and Azide free (AB236144)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Nuclear staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202063).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-Rad51D antibody [EPR16205] - BSA and Azide free (AB236144)

Rad51D was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab202063 at 1/50 dilution.

Western blot was performed from the immunoprecipitate using ab202063 at 1/1000 dilution.

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.

Lane 1 : HeLa whole cell lysate 10 µg (Input).

Lane 2 : ab202063 IP in HeLa whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab202063 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202063).

All lanes:

Immunoprecipitation - Anti-Rad51D antibody [EPR16205] (<a href='/ko/products/primary-antibodies/rad51d-antibody-epr16205-ab202063'>ab202063</a>)

Predicted band size: 35 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51D antibody [EPR16205] - BSA and Azide free (AB236144)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Rad51D with ab202063 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Nuclear staining on rat kidney tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202063).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Rad51D antibody [EPR16205] - BSA and Azide free (AB236144)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202063).

Western blot : Anti-Rad51D antibody [EPR16205] ab202063 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 35 kDa in Wild-type HCT 116 cell lysates with no signal observed at this size in RAD51D knockout HCT 116 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent Western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-0.1 % Tween^® 20 (TBS-T), incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Rad51D antibody [EPR16205] (<a href='/ko/products/primary-antibodies/rad51d-antibody-epr16205-ab202063'>ab202063</a>) at 1/1000 dilution

Lane 1:

Wild-type HCT 116 cell lysates at 20 µg

Lane 2:

Western blot - Human RAD51D knockout HCT116 cell line (<a href='/ko/products/cell-lines/human-rad51d-knockout-hct116-cell-line-ab286478'>ab286478</a>) at 20 µg

Lane 3:

HeLa cell lysates at 20 µg

Lane 4:

Calu-3 cell lysates at 20 µg

Lane 5:

SH-SY5Y cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 35 kDa

Observed band size: 35 kDa

false