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AB97659

Anti-RanBP1 antibody

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(11 제품이 사용된 논문 )

Rabbit Polyclonal RanBP1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RANBP1 aa 1-200.
2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB97659)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB97659)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Ran BP1 protein at cytoplasm using ab97659 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/10000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Western blot - Anti-RanBP1 antibody (AB97659)
  • WB

Supplier Data

Western blot - Anti-RanBP1 antibody (AB97659)

Samples were separated by 12% SDS PAGE.

All lanes:

Western blot - Anti-RanBP1 antibody (ab97659) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 50 µg

Predicted band size: 24 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human RANBP1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P43487

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대체품

Primary Antibodies

AB133550

Anti-RanBP1 antibody [EPR6796(B)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-RanBP1 antibody [EPR6796(B)] (AB133550)

  • 0
  • 0
Primary Antibodies

AB217940

Anti-RanBP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP1 antibody (AB217940)

  • 0
  • 0
Primary Antibodies

AB2937

Anti-RanBP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB2937)

  • 0
  • 0
Primary Antibodies

AB264129

Anti-RanBP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP1 antibody (AB264129)

  • 0
  • 0
Primary Antibodies

AB70185

Anti-RanBP1 antibody

Immunoprecipitation - Anti-RanBP1 antibody (AB70185)

  • 0
  • 0
Primary Antibodies

AB17034

Anti-RanBP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RanBP1 antibody (AB17034)

  • 0
  • 0

보완 제품

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB191875

Anti-DGCR8 antibody [EPR18757]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-DGCR8 antibody [EPR18757] (AB191875)

  • 5
  • 2
Primary Antibodies

AB316336

Anti-RanBP2 antibody [RM2047]

BOND RX™ Validated|20ul selling size|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP2 antibody [RM2047] (AB316336)

  • 0
  • 0
Primary Antibodies

AB133741

Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741)

  • 4
  • 15
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RanBP1 also known as Ran-binding protein 1 is an important player in the regulation of the Ran GTPase cycle integral to transport processes across the nuclear envelope. This protein has a mass of about 23 kDa. In humans RanBP1 shows expression in various tissues with higher levels observed in testis and brain. It directly interacts with Ran a small GTPase helping to modulate its activity by promoting the conversion between the active GTP-bound form and the inactive GDP-bound form.
Biological function summary

RanBP1 functions closely with Ran GTPase in the nucleocytoplasmic transport system where it is part of a complex with Ran and other factors like importins and exportins. It aids in the stabilization of the Ran-GTPase complex facilitating the release of cargo molecules and ensuring efficient transport cycles within the cells. RanBP1 in conjunction with Ran regulates the dynamic transport of proteins and RNA essential for maintaining cellular compartmentalization.

Pathways

The RanBP1 protein plays a role in the Ran GTPase cycle and nucleocytoplasmic transport pathways. These pathways are essential to cellular dynamics affecting overall transport between the cytoplasm and the nucleus. Furthermore RanBP1 affects interactions with proteins such as RanGAP1 contributing to the regulation of Ran activity cycles and supporting cellular functions including mitosis and RNA processing.

Research indicates that alterations in RanBP1 expression and function relate to cancer progression and neurodegenerative conditions. Misregulation of RanBP1 can affect nuclear transport balance impacting cancer-promoting pathways. Its interaction with Ran and its pathway-related proteins like importin beta suggests a role in tumorigenesis by contributing to aberrant cell cycle regulation and mitotic errors while its importance in neuron function suggests involvement in neurodegenerative disease mechanisms.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Plays a role in RAN-dependent nucleocytoplasmic transport. Alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus (By similarity). Induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PubMed : 20485264). Promotes the disassembly of the complex formed by RAN and importin beta. Promotes dissociation of RAN from a complex with KPNA2 and CSE1L (By similarity). Required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PubMed : 17671426). Does not increase the RAN GTPase activity by itself, but increases GTP hydrolysis mediated by RANGAP1 (PubMed : 7882974). Inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PubMed : 7616957, PubMed : 7882974).
See full target information RANBP1

대체 명칭 보기

Ran-specific GTPase-activating protein, Ran-binding protein 1, RanBP1, RANBP1
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제품이 사용된 논문 (11)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 16:6548 PubMed40670348

2025

Elaboration of molecular glues that target TRIM21 into TRIMTACs that degrade protein aggregates.

Applications

Unspecified application

Species

Unspecified reactive species

Marc A Scemama de Gialluly,Anthony R Allen,Elijah H Hayes,Patrick Zhuang,Ralston B Goldfarb,Amanda N Farrar,Yuriy Fedorov,Drew J Adams

eLife 13: PubMed40420562

2025

An antisense oligonucleotide-based strategy to ameliorate cognitive dysfunction in the 22q11.2 Deletion Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Pratibha Thakur,Martin Lackinger,Anastasia Diamantopoulou,Sneha Rao,Yijing Chen,Khakima Khalizova,Annie Ferng,Curt Mazur,Holly Kordasiewicz,Robert J Shprintzen,Sander Markx,Bin Xu,Joseph A Gogos

Cell chemical biology 31:1363-1372.e8 PubMed38917791

2024

C646 degrades Exportin-1 to modulate p300 chromatin occupancy and function.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Fan Chen,Atikur Rahman,Joel L Sax,Matthew J Atala Pleshinger,Ryan M Friedrich,Drew J Adams

Human molecular genetics 32:1959-1974 PubMed36790128

2023

Ranbp1 modulates morphogenesis of the craniofacial midline in mouse models of 22q11.2 deletion syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth M Paronett,Corey A Bryan,Megan E Maynard,Julia A Goroff,Daniel W Meechan,Anthony-Samuel LaMantia,Thomas M Maynard

Pharmaceuticals (Basel, Switzerland) 14: PubMed34832913

2021

Inhibition of XPO-1 Mediated Nuclear Export through the Michael-Acceptor Character of Chalcones.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Gargantilla,José López-Fernández,Maria-Jose Camarasa,Leentje Persoons,Dirk Daelemans,Eva-Maria Priego,María-Jesús Pérez-Pérez

Cell reports 24:2529-2539.e4 PubMed30184488

2018

RanBP1 Couples Nuclear Export and Golgi Regulation through LKB1 to Promote Cortical Neuron Polarity.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Mencarelli,Justyna Nitarska,Tim Kroecher,Francesco Ferraro,Katherine Massey,Antonella Riccio,Franck Pichaud

Cellular signalling 46:76-82 PubMed29501729

2018

STAT6 is a cargo of exportin 1: Biological relevance in primary mediastinal B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hadjer Miloudi,Karen Leroy,Fabrice Jardin,Brigitte Sola

Cell death & disease 7:e2497 PubMed27906185

2016

Anti-proliferative activity of the NPM1 interacting natural product avrainvillamide in acute myeloid leukemia.

Applications

WB

Species

Human

Vibeke Andresen,Bjarte S Erikstein,Herschel Mukherjee,André Sulen,Mihaela Popa,Steinar Sørnes,Håkon Reikvam,Kok-Ping Chan,Randi Hovland,Emmet McCormack,Øystein Bruserud,Andrew G Myers,Bjørn T Gjertsen

Oncotarget 7:68842-68850 PubMed27634897

2016

Heterozygous mutation of cysteine528 in XPO1 is sufficient for resistance to selective inhibitors of nuclear export.

Applications

Unspecified application

Species

Unspecified reactive species

Jasper Edgar Neggers,Els Vanstreels,Erkan Baloglu,Sharon Shacham,Yosef Landesman,Dirk Daelemans

Chemistry & biology 22:107-16 PubMed25579209

2015

Identifying drug-target selectivity of small-molecule CRM1/XPO1 inhibitors by CRISPR/Cas9 genome editing.

Applications

ICC/IF

Species

Human

Jasper E Neggers,Thomas Vercruysse,Maarten Jacquemyn,Els Vanstreels,Erkan Baloglu,Sharon Shacham,Marsha Crochiere,Yosef Landesman,Dirk Daelemans
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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