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AB281172

Anti-RANKL antibody [EPR24036-149] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal RANKL antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

대체 명칭 보기

CD254, OPGL, RANKL, TRANCE, TNFSF11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF

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Sandwich ELISA - Anti-RANKL antibody [EPR24036-149] - BSA and Azide free (Capture) (AB281172)
  • sELISA

Lab

Sandwich ELISA - Anti-RANKL antibody [EPR24036-149] - BSA and Azide free (Capture) (AB281172)

Sandwich ELISA of ab253612 with the capture antibody ab281172 dilution at 2 µg/mL and detector antibody ab281022 dilution at 0.5 µg/mL.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24036-149

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB213841

Human TNFSF11 ELISA Kit (RANKL)

Sandwich ELISA - Human TNFSF11 ELISA Kit (RANKL) (AB213841)

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Primary Antibodies

AB259515

Anti-TNF Receptor II antibody [EPR22388-260] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-TNF Receptor II antibody [EPR22388-260] - BSA and Azide free (Capture) (AB259515)

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Primary Antibodies

AB278014

Anti-RANK antibody [EPR23308-30] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-RANK antibody [EPR23308-30] - BSA and Azide free (Detector) (AB278014)

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Primary Antibodies

AB303698

Anti-SLAM / CD150 antibody [EPR25896-92] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-SLAM / CD150 antibody [EPR25896-92] - BSA and Azide free (Capture) (AB303698)

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Primary Antibodies

AB242932

Anti-S100A9 antibody [EPR20066-233] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-S100A9 antibody [EPR20066-233] - BSA and Azide free (Detector) (AB242932)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab281172 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human TNFSF11. The recommended pair for sandwich ELISA is:
Capture: ab281172, Human TNFSF11 Capture Antibody (unconjugated)
Detector: ab281022, Human TNFSF11 Detector Antibody (unconjugated)
The reference range value is 7.81-1000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed : 22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).
See full target information TNFSF11
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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