AB173296

Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)]

RabMAb
Recombinant
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal RAP2A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

대체 명칭 보기

Ras-related protein Rap-2a, RbBP-30, RAP2A, Ras-related protein Rap-2c, RAP2C, Ras-related protein Rap-2b, RAP2B

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RAP2 with ab173296 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

Immunofluorescent analysis of MCF7 cells labeling RAP2 with ab173296 at 1/50 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling RAP2 with ab173296 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

All lanes:

Western blot - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (ab173296) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Predicted band size: 21 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12825(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Rap2A Rap2B and Rap2C are part of the Ras-related protein subfamily often called small GTPases. These proteins act as molecular switches that cycle between active GTP-bound and inactive GDP-bound states. The mass of these proteins is approximately 21 kilodaltons each. They are expressed in various tissues including the brain where they participate in signaling pathways critical for cellular communication. The three proteins share similarities in structure and function yet have unique roles in their specific cellular contexts.

Biological function summary

Rap2A Rap2B and Rap2C are involved in regulating cell migration adhesion and morphogenesis. As GTPases they interact with downstream effectors to modulate cytoskeletal dynamics and vesicle trafficking. These proteins are not part of a single complex but work collaboratively with various signaling proteins to transmit signals from the cell surface to the interior.

Pathways

Rap2 proteins play a role in the MAPK/ERK and PI3K/Akt signaling pathways. These pathways are important for cell growth survival and differentiation. They interact with proteins such as c-Raf and Akt transmitting external signals to influence cellular responses. Rap2 proteins contribute to the fine-tuning of these pathways affecting how cells respond to external stimuli.

Aberrant Rap2 signaling associates with cancer and neuronal disorders. Overexpression or mutations can lead to tumorigenic processes by affecting cell proliferation and survival mechanisms. Additionally their misregulation links to neurodegenerative conditions due to their role in neuronal signaling and plasticity. Other proteins like Ras and RhoA interact with Rap2 proteins in these pathological conditions indicating their importance in disease pathways.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form (PubMed : 14966141, PubMed : 15342639, PubMed : 16246175, PubMed : 16540189, PubMed : 18930710, PubMed : 20159449, PubMed : 35293963). In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK (PubMed : 14966141, PubMed : 15342639, PubMed : 18930710, PubMed : 20159449). Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development (PubMed : 20159449). More generally, it is part of several signaling cascades and regulates cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading (PubMed : 14966141, PubMed : 15342639, PubMed : 16246175, PubMed : 16540189, PubMed : 18930710, PubMed : 20159449, PubMed : 35293963).

See full target information Ras-related protein Rap-2a

Additional targets

Ras-related protein Rap-2b,Ras-related protein Rap-2c

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

BMC molecular and cell biology 26:6 PubMed39838328

2025

BRAF regulates circPSD3/miR-526b/RAP2A axis to hinder papillary thyroid carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Li,Xiaojuan Zhao,Jingge Zhao,Jing Zhao,Lemei An,Gang Wu

Oncology reports 40:179-194 PubMed29781035

2018

Knockdown of KPNA2 inhibits autophagy in oral squamous cell carcinoma cell lines by blocking p53 nuclear translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Lin,Li Gao,Zhenyu Su,Xiaofang Cao,Yuanbo Zhan,Ying Li,Bin Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

Immunocytochemistry/ Immunofluorescence - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

Western blot - Anti-Rap2A+Rap2B+Rap2C antibody [EPR12825(B)] (AB173296)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Additional targets

제품이 사용된 논문 (2)

Product promise