Anti-RASA2 antibody [EPR29503-39]

• WB

Supplier Data

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : skeletal muscle

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-RASA2 antibody [EPR29503-39] (ab323554) at 1/1000 dilution

Lane 1:

Human thyroid tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 96 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : PC-3

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-RASA2 antibody [EPR29503-39] (ab323554) at 1/1000 dilution

Lane 1:

DLD-1 (human colorectal adenocarcinoma epithelial cell) whole lysate at 20 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell) whole lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 96 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1-4 : 92 seconds, lane 5 : 114 seconds

All lanes:

Western blot - Anti-RASA2 antibody [EPR29503-39] (ab323554) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell) whole lysate at 20 µg

Lane 2:

HFF-1 (human skin fibroblast) whole lysate at 20 µg

Lane 3:

A431 (human epidermoid carcinoma epithelial cell) whole lysate at 20 µg

Lane 4:

NCI-H1299 (human lung carcinoma epithelial cell) whole lysate at 20 µg

Lane 5:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution

Observed band size: 96 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human RASA3 by western blot.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-RASA2 antibody [EPR29503-39] (ab323554) at 1/1000 dilution

Lane 1:

His-tagged human RASA2 recombinant protein at 10 ng

Lane 2:

His-tagged human RASA3 recombinant protein at 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 96 kDa,33 kDa

false

Exposure time: 15s