AB121577

Anti-RASGRF2 antibody

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(4 제품이 사용된 논문 )

Rabbit Polyclonal RASGRF2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RASGRF2 aa 700-850.

대체 명칭 보기

GRF2, RASGRF2, Ras-specific guanine nucleotide-releasing factor 2, Ras-GRF2, Ras guanine nucleotide exchange factor 2

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)

Immunohistochemical analysis of human prostate tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in glandular cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)

Immunohistochemical analysis of human cerebral cortex tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in neuronal cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)

Immunohistochemical analysis of human placenta tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in trophoblastic cells.

Immunocytochemistry/ Immunofluorescence - Anti-RASGRF2 antibody (AB121577)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RASGRF2 antibody (AB121577)

ab121577, at 4 μg/ml, staining RASGRF2 in PFA/Triton X-100 treated Human cell line A-431 by immunofluorescence. ab121577 staining is shown in green.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)

Immunohistochemical analysis of human testis tissue labelled for RASGRF2 using ab121577. Positive cytoplasmic staining is observed in cells in the seminiferous ducts.

Unknown

Western blot - Anti-RASGRF2 antibody (AB121577)

All lanes:

Western blot - Anti-RASGRF2 antibody (ab121577) at 1/250 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 141 kDa

true

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RASGRF2 aa 700-850. The exact immunogen used to generate this antibody is proprietary information.

O14827

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "Use buffer at pH 6 for antigen retrieval. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/500", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation. Functions in synaptic plasticity by contributing to the induction of long term potentiation.

See full target information RASGRF2

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of Cancer 12:7177-7189 PubMed34729119

2021

High Expression of ERK-related RASGRF2 predicts Poor prognosis in patients with Stomach Adenocarcinoma and correlates with M2 macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqi Du,Zhengguang Wang,Weina Wan

Pigment cell & melanoma research 34:1074-1083 PubMed34310073

2021

RASGRF2 gene fusions identified in a variety of melanocytic lesions with distinct morphological features.

Applications

Unspecified application

Species

Unspecified reactive species

Aurélie Houlier,Daniel Pissaloux,Franck Tirode,Noémie Lopez Ramirez,Maud Plaschka,Julie Caramel,Ingrid Masse,Arnaud de la Fouchardiere

PloS one 15:e0231711 PubMed32298357

2020

Reciprocal expression of Annexin A6 and RasGRF2 discriminates rapidly growing from invasive triple negative breast cancer subsets.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Y Korolkova,Sarrah E Widatalla,Diva S Whalen,Gladys N Nangami,Adeniyi Abimbola,Stephen D Williams,Heather K Beasley,Emily Reisenbichler,Mary Kay Washington,Josiah Ochieng,Ingrid A Mayer,Brian D Lehmann,Amos M Sakwe

Cancer biology & therapy 20:435-443 PubMed30359168

2018

RasGRF2 promotes migration and invasion of colorectal cancer cells by modulating expression of MMP9 through Src/Akt/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peifen Lu,Junjun Chen,Lihong Yan,Lijun Yang,Litao Zhang,Jie Dai,Zixuan Hao,Tao Bai,Yanfeng Xi,Yahui Li,Zhiming Kang,Jun Xv,Gongqin Sun,Tao Yang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RASGRF2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)

Immunocytochemistry/ Immunofluorescence - Anti-RASGRF2 antibody (AB121577)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASGRF2 antibody (AB121577)

Western blot - Anti-RASGRF2 antibody (AB121577)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise