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AB184702

Anti-Rb (phospho S780) antibody [EPR17624]

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal RB phospho S780 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Synthetic peptide samples. Cited in 3 publications.

대체 명칭 보기

Retinoblastoma-associated protein, p105-Rb, p110-RB1, pRb, pp110, Rb, RB1

6 이미지
Immunoprecipitation - Anti-Rb (phospho S780) antibody [EPR17624] (AB184702)
  • IP

Supplier Data

Immunoprecipitation - Anti-Rb (phospho S780) antibody [EPR17624] (AB184702)

Rb (phospho S780) was immunoprecipitated from 0.35 mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab184702 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : K562 whole cell lysate,10μg (Input).

Lane 2 : ab184702 IP in K562 whole cell lysate.

Lane 3 : Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184702 in K562 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)

Predicted band size: 106 kDa

false

Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (AB184702)
  • WB

Supplier Data

Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (AB184702)

Blocking/Dilution buffer : 5% NFDM/TBST.

Rb phosphorylation at S780 is regulated by cell cycle progression, which is consistent with what has been described in the literature (PMID : 11134518 and 24765199).

The lysates were prepared in 1%SDS Hot lysis method

All lanes:

Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/1000 dilution

Lane 1:

Untreated K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg

Lane 2:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate serum starved for 2 days at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 106 kDa

Observed band size: 106 kDa

false

Exposure time: 10s

Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (AB184702)
  • WB

Supplier Data

Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (AB184702)

Blocking/Dilution buffer : 5% NFDM/TBST.

The lysates were prepared in 1%SDS Hot lysis method

All lanes:

Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/1000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg

Lane 2:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate treated with Alkaline Phosphatase for 1 hour at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 106 kDa

Observed band size: 106 kDa

false

Exposure time: 30s

Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (AB184702)
  • WB

Unknown

Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (AB184702)

The lysates were prepared in 1%SDS Hot lysis method

Observed MW : 106 kDa

blocking/diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/5000 dilution

Lane 1:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates prepared in RIPA lysis method at 15 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 106 kDa

false

Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (AB184702)
  • WB

Supplier Data

Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (AB184702)

Blocking/Dilution buffer : 5% NFDM/TBST.

The lysates were prepared in 1%SDS Hot lysis method

All lanes:

Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/5000 dilution

Lane 1:

L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate at 10 µg

Lane 2:

L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate treated with Alkaline Phosphatase for 1 hour at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 101 kDa,106 kDa

Observed band size: 102 kDa,106 kDa

false

Exposure time: 3min

Dot Blot - Anti-Rb (phospho S780) antibody [EPR17624] (AB184702)
  • Dot

Supplier Data

Dot Blot - Anti-Rb (phospho S780) antibody [EPR17624] (AB184702)

Dot blot analysis of Rb (phospho S780) labeled with ab184702 at 1/1000 dilution.

Lane 1 : Rb (phospho S780) phospho peptide;

Lane 2 : Rb non-phospho peptide.

Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1/100000 was used as secondary antibody.

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-Rb (phospho S780) antibody [EPR17624] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17624

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Dot, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB277935

Alexa Fluor® 594 Anti-Rb (phospho S807) antibody [EPR17732]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Rb (phospho S807) antibody [EPR17732] (AB277935)

  • 0
  • 0
Primary Antibodies

AB181616

Anti-Rb antibody [EPR17512]

20ul selling size|KO Validated|RabMAb|Recombinant|Advanced Validation

Western blot - Anti-Rb antibody [EPR17512] (AB181616)

  • 0
  • 0
Primary Antibodies

AB173289

Anti-Rb (phospho S780) antibody [EPR182(N)]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody [EPR182(N)] (AB173289)

  • 5
  • 1
Primary Antibodies

AB218526

Anti-Rb antibody [EPR17512] - BSA and Azide free

KO Validated|RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb antibody [EPR17512] - BSA and Azide free (AB218526)

  • 0
  • 0
Primary Antibodies

AB184796

Anti-Rb (phospho S807) antibody [EPR17732]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Rb (phospho S807) antibody [EPR17732] (AB184796)

  • 5
  • 2

보완 제품

Primary Antibodies

AB181616

Anti-Rb antibody [EPR17512]

20ul selling size|KO Validated|RabMAb|Recombinant|Advanced Validation

Western blot - Anti-Rb antibody [EPR17512] (AB181616)

  • 0
  • 0
Primary Antibodies

AB133446

Anti-Rb (phospho T826) antibody [EPR5351]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-Rb (phospho T826) antibody [EPR5351] (AB133446)

  • 0
  • 0
Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

  • 5
  • 104
Primary Antibodies

AB184796

Anti-Rb (phospho S807) antibody [EPR17732]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Rb (phospho S807) antibody [EPR17732] (AB184796)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Retinoblastoma protein (Rb) also known as pRb is an important regulatory protein with a molecular weight of approximately 110 kDa. It is mainly expressed in the nucleus of cells. Rb functions as a tumor suppressor by controlling the cell cycle progression from G1 to S phase. Rb becomes active when it is dephosphorylated allowing it to bind and inhibit E2F transcription factors consequently preventing the transcription of genes essential for S phase entry.
Biological function summary

Retinoblastoma protein influences cellular proliferation differentiation and apoptosis. It acts within a larger protein complex modulating various cellular responses. When functional Rb halts uncontrolled cell division important for maintaining normal tissue homeostasis. In its phosphorylated state known as phospho-Rb or phospho-Rb E182 it loses its regulatory capabilities which can lead to unrestrained cell cycle progression.

Pathways

Several involve the retinoblastoma protein. One key pathway is the p53 pathway which Rb interacts with to influence cellular outcomes like cell-cycle arrest and apoptosis. Rb cooperates with proteins like p21 to implement these processes. Additionally it is involved in the cyclin D-dependent kinase 4 (CDK4) pathway which modulates its phosphorylation state influencing binding interactions and cell cycle control.

Dysregulation of retinoblastoma protein is commonly associated with cancer particularly retinoblastoma and breast cancer. In retinoblastoma mutations in the Rb gene directly lead to uncontrolled cell proliferation due to the absence of functional Rb protein. Additionally Rb's connection to the E2F family of transcription factors can become disrupted contributing to oncogenesis in other cancers.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed : 10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed : 10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed : 10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed : 10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed : 15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity).. (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
See full target information RB1 pS780
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

CNS neuroscience & therapeutics 31:e70608 PubMed40955046

2025

Gut Microbiota-Bile Acid-Brain Axis and TGR5-ERK1/2 Signaling Mediate ADT-Induced Cognitive Impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Yanbo Liu,Zhien Zhou,Dong Yang,Weigang Yan

Journal of translational medicine 20:264 PubMed35676661

2022

C2orf40 inhibits metastasis and regulates chemo-resistance and radio-resistance of nasopharyngeal carcinoma cells by influencing cell cycle and activating the PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zuozhong Xie,Wei Li,Jingang Ai,Jun Xie,Xiaowei Zhang

Cellular and molecular gastroenterology and hepatology : PubMed33484913

2021

p53-independent induction of p21 fails to control regeneration and hepatocarcinogenesis in a murine liver injury model.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Elisa Buitrago-Molina,Silke Marhenke,Diana Becker,Robert Geffers,Timo Itzel,Andreas Teufel,Hartmut Jaeschke,André Lechel,Kristian Unger,Jovana Markovic,Amar Deep Sharma,Jens U Marquardt,Michael Saborowski,Anna Saborowski,Arndt Vogel
제품이 사용된 논문 모두 보기
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Product promise

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