AB150822

Anti-RBM28 antibody

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Rabbit Polyclonal RBM28 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBM28 aa 350 to C-terminus.

대체 명칭 보기

RNA-binding protein 28, RNA-binding motif protein 28, RBM28

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM28 antibody (AB150822)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM28 antibody (AB150822)

Immunohistochemical analysis of paraffin-embedded Human hippocampus tissue labelling RBM28 with ab150822 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-RBM28 antibody (AB150822)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RBM28 antibody (AB150822)

Immunofluorescent analysis of Human cell line U-2 OS, labelling RBM28 with ab150822 at 1/50 (green). Red staining marks microtubules labelled with an anti-tubulin antibody.

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Western blot - Anti-RBM28 antibody (AB150822)

All lanes:

Western blot - Anti-RBM28 antibody (ab150822) at 1/250 dilution

Lane 1:

RT 4 cell lysate

Lane 2:

U 251 MG cell lysate

Lane 3:

IgG/HSA-depleted Human plasma

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 85 kDa

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Western blot - Anti-RBM28 antibody (AB150822)

Lane 1 : NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)

All lanes:

Western blot - Anti-RBM28 antibody (ab150822)

Predicted band size: 85 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human RBM28 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NW13

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab150822 should be gently mixed before use.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RBM28 also known as RNA-binding motif protein 28 weighs approximately 97 kDa. This protein resides primarily in the nucleolus of cells where it plays key mechanical roles in RNA processing events. As an RNA-binding protein RBM28 binds specifically to snoRNA pre-rRNA and possibly other nucleolar transcripts. It is expressed in various tissues with high expression levels in the brain heart and skeletal muscles indicating its widespread importance in cellular functions.

Biological function summary

RBM28 functions as an essential component of the ribonucleoprotein complexes. It associates with small nucleolar RNAs and pre-ribosomal particles facilitating the maturation of rRNA and the assembly of ribosomes. By engaging in these processes RBM28 ensures the proper synthesis and assembly of ribosomal subunits which are important for protein synthesis in cells. The protein partners with others such as NOP56 and FBL in processing pre-rRNA to mature forms.

Pathways

RBM28 plays a significant role in ribosome biogenesis and RNA metabolic processing. Its involvement in the biogenesis of ribosomes places it within the broader gene expression pathway. The protein interacts with others like DDX21 and nucleolin within this pathway showing its intricate connection to the production and maturation of ribosomes. Such associations help maintain the balance of ribosome production ensuring cells adapt and respond to their translational needs.

RBM28 mutations or dysfunctions relate to conditions like alopecia and neurological disorders. Notably mutations in RBM28 have been linked to alopecia neurological defects and endocrinopathy syndrome indicating the protein's role in hair brain and endocrine system function. The protein's interactions with nucleolin and FBL suggest it might influence developmental pathways affecting neurological and hair follicle homeostasis. Understanding these connections can help in deciphering pathophysiological mechanisms underlying these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Nucleolar component of the spliceosomal ribonucleoprotein complexes.

See full target information RBM28

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

JCI insight 9: PubMed38194286

2024

Extended time frame for restoring inner ear function through gene therapy in Usher1G preclinical model.

Applications

Unspecified application

Species

Unspecified reactive species

Ghizlene Lahlou,Charlotte Calvet,François Simon,Vincent Michel,Lauranne Alciato,Baptiste Plion,Jacques Boutet de Monvel,Marie-José Lecomte,Mathieu Beraneck,Christine Petit,Saaid Safieddine

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RBM28 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM28 antibody (AB150822)

Immunocytochemistry/ Immunofluorescence - Anti-RBM28 antibody (AB150822)

Western blot - Anti-RBM28 antibody (AB150822)

Western blot - Anti-RBM28 antibody (AB150822)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise