Anti-RBM47 antibody [EPR9658]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM47 antibody [EPR9658] (AB167164)

ab167164 showing +ve staining in Human thyroid gland .

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM47 antibody [EPR9658] (AB167164)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling RBM47 with ab167164 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM47 antibody [EPR9658] (AB167164)

ab167164 showing +ve staining in Human normal uterus.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM47 antibody [EPR9658] (AB167164)

ab167164 showing +ve staining in Human prostate hyperplasia.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM47 antibody [EPR9658] (AB167164)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling RBM47 with ab167164 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM47 antibody [EPR9658] (AB167164)

ab167164 showing +ve staining in Human lung carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Unknown

Western blot - Anti-RBM47 antibody [EPR9658] (AB167164)

All lanes:

Western blot - Anti-RBM47 antibody [EPR9658] (ab167164) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

Caco-2 cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Lane 5:

RAW 264.7 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 64 kDa

false

• WB

CiteAb

Western blot - Anti-RBM47 antibody [EPR9658] (AB167164)

Western Blotting using Anti-RBM47 antibody [EPR9658], ab167164. Publication image from Teupser, D. et al., 2016, Nat Commun, 27665711. Legend direct from paper.

Correlation of vigilin levels with liver steatosis and plasma lipid levels.(a) Western blot analysis of vigilin in mouse tissues. (b) Nuclear and cytoplasmic fractions from primary mouse hepatocytes. (c) Immunoblot analysis of hepatic vigilin levels in 20-week-old chow-fed (Chow) or high-fat diet C57BL/6 (DIO) and in 23 week old ob/ob mice. Correlation between hepatic vigilin expression quantified by densitometry from c (n=6 per group) and (d) plasma triglyceride, (e) non-esterified fatty acid (NEFA) and (f) cholesterol levels. Correlation between (g) degree of hepatic steatosis and (h) clinical/histological diagnosis with hepatic vigilin expression quantified by immunoblotting and densitometry from human liver biopsies, including 5 healthy, 10 non-alcoholic fatty liver disease (NAFLD) and 10 non-alcoholic steatohepatitis (NASH) patients. Values in h are expressed as mean±s.d. *P≤0.05, **P≤0.01, ***P≤0.001; P values and R2 were determined by two-tailed Pearson's correlation test (in d–g) or ANOVA with Holm-Sidak post hoc analysis (in h).

false