AB245646

Anti-RBM5 antibody

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(6 제품이 사용된 논문 )

Rabbit Polyclonal RBM5 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human RBM5 aa 750 to C-terminus.

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Supplier Data

Immunoprecipitation - Anti-RBM5 antibody (AB245646)

RBM5 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

ab245646 used for IP at 10 μg/mg lysate. For WB 1 μg/ml.

Lane 1 : ab245646 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-RBM5 antibody (ab245646)

Predicted band size: 92 kDa

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Supplier Data

Western blot - Anti-RBM5 antibody (AB245646)

All lanes:

Western blot - Anti-RBM5 antibody (ab245646) at 0.4 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 92 kDa

false

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RBM5 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P52756

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab245646 was affinity purified using an epitope specific to RBM5 immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RBM5 also known as RNA-binding motif protein 5 or LUCA-15 plays a significant role in pre-mRNA splicing regulation. Its molecular mass is approximately 94 kDa. This protein is expressed in various tissues with noticeable levels in the heart liver and lung. RBM5 influences splice site selection during RNA processing impacting gene expression patterns.

Biological function summary

RBM5 functions as a tumor suppressor influencing cell cycle regulation and apoptosis. It forms part of a spliceosomal complex playing a critical role in the alternative splicing of its target mRNAs. This activity is essential for maintaining normal cellular homeostasis especially in rapidly dividing cells. RBM5 interacts with other splicing factors to modulate the inclusion of specific exons impacting overall cell function.

Pathways

The regulation of alternative splicing by RBM5 integrates into the apoptotic and cell cycle pathways. Its interaction with proteins like Fas and Bcl-2 highlights its role in managing programmed cell death. These connections make RBM5 central in ensuring the balance between cell survival and death an important element in cancer suppression. It also collaborates with other splicing proteins in the spliceosomal pathway to coordinate precise RNA maturation.

Mutations or altered expression of RBM5 link to cancer notably lung and breast cancer. Its tumor-suppressive effects in these conditions highlight its importance in maintaining normal cell behavior. Moreover RBM5 interacts with p53 a well-known tumor suppressor protein indicating its coordinated role in preventing aberrant cell proliferation. The dysregulation of RBM5 and subsequent disruption in RNA processing highlights its significance in cancer-related pathways.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the spliceosome A complex. Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3' (PubMed : 21256132). Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate apoptosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.

See full target information RBM5

대체 명칭 보기

H37, LUCA15, RBM5, RNA-binding protein 5, Protein G15, Putative tumor suppressor LUCA15, RNA-binding motif protein 5, Renal carcinoma antigen NY-REN-9

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

World journal of gastrointestinal oncology 16:3241-3255 PubMed39072172

2024

RBM5 suppresses proliferation, metastasis and glycolysis of colorectal cancer cells stabilizing phosphatase and tensin homolog mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Chu-Xiang Wang,Feng Liu,Yi Wang

Science advances 10:eadn7732 PubMed38809976

2024

DNMT3B splicing dysregulation mediated by SMCHD1 loss contributes to DUX4 overexpression and FSHD pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Eden Engal,Aveksha Sharma,Uria Aviel,Nadeen Taqatqa,Sarah Juster,Shiri Jaffe-Herman,Mercedes Bentata,Ophir Geminder,Adi Gershon,Reyut Lewis,Gillian Kay,Merav Hecht,Silvina Epsztejn-Litman,Marc Gotkine,Vincent Mouly,Rachel Eiges,Maayan Salton,Yotam Drier

European journal of haematology 110:498-509 PubMed36536516

2023

RNA-binding protein PUM2 promotes T-cell acute lymphoblastic leukemia via competitively binding to RBM5 3'UTR with miR-28-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Taomin Bai,Na Liu

Cancer cell 41:164-180.e8 PubMed36563682

2022

Modulation of RNA splicing enhances response to BCL2 inhibition in leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Wang,Jose Mario Bello Pineda,Won Jun Kim,Sisi Chen,Jessie Bourcier,Maximilian Stahl,Simon J Hogg,Jan Phillipp Bewersdorf,Cuijuan Han,Michael E Singer,Daniel Cui,Caroline E Erickson,Steven M Tittley,Alexander V Penson,Katherine Knorr,Robert F Stanley,Jahan Rahman,Gnana Krishnamoorthy,James A Fagin,Emily Creger,Elizabeth McMillan,Chi-Ching Mak,Matthew Jarvis,Carine Bossard,Darrin M Beaupre,Robert K Bradley,Omar Abdel-Wahab

Disease markers 2022:8402116 PubMed36193508

2022

hsa_circ_0003176 Suppresses the Progression of Non-Small-Cell Lung Cancer via Regulating miR-182-5p/RBM5 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fangfang Yang,YanLi Pei,Wei Xu,Lei Rong

Nature 591:E28 PubMed33707632

2021

Author Correction: The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Seifert,Gregor Werba,Shaun Tiwari,Nancy Ngoc Giao Ly,Sara Alothman,Dalia Alqunaibit,Antonina Avanzi,Rocky Barilla,Donnele Daley,Stephanie H Greco,Alejandro Torres-Hernandez,Matthew Pergamo,Atsuo Ochi,Constantinos P Zambirinis,Mridul Pansari,Mauricio Rendon,Daniel Tippens,Mautin Hundeyin,Vishnu R Mani,Cristina Hajdu,Dannielle Engle,George Miller

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RBM5 antibody

Immunoprecipitation - Anti-RBM5 antibody (AB245646)

Western blot - Anti-RBM5 antibody (AB245646)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise