AB31106

Anti-RBP1 antibody

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(2 제품이 사용된 논문 )

Goat Polyclonal RET1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RBP1 aa 100-150.

대체 명칭 보기

CRBP1, RBP1, Retinol-binding protein 1, Cellular retinol-binding protein, Cellular retinol-binding protein I, CRBP, CRBP-I

1 이미지

Immunocytochemistry/ Immunofluorescence - Anti-RBP1 antibody (AB31106)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RBP1 antibody (AB31106)

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr with ab31106 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml). The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human RBP1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P09455

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Ab31106 underwent ammonium sulphate precipitation prior to antigen affinity chromatography purification.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Retinol binding protein 1 (RBP1) also known as cellular retinol-binding protein 1 (CRBP1) plays a critical role in the regulation and transportation of retinol a form of vitamin A within cells. RBP1 has a molecular mass of approximately 15 kDa and localizes predominantly in the cytoplasm. It is expressed in multiple tissues including the liver kidney and adipose tissue where it helps in regulating retinoid metabolism. The presence of RBP1 in these tissues is essential for maintaining physiological retinol levels.

Biological function summary

RBP1 assists in the cellular uptake storage and metabolism of retinol facilitating its conversion into retinal and retinoic acid. Although it is not part of a well-defined complex RBP1 works closely with enzymes involved in retinol metabolism such as lecithin retinol acyltransferase (LRAT) and retinal dehydrogenases. This process is important for maintaining visual function immune response and cellular differentiation which all rely on proper retinol metabolism and activity.

Pathways

RBP1 plays a significant role in the retinoic acid signaling pathway affecting gene expression regulation by modulating retinoid concentrations. It interacts with retinoic acid receptor (RAR) and retinoid X receptor (RXR) pathways influencing transcriptional activities related to development and differentiation. Connections between RBP1 and LRAT are important for the retinol esterification process integrating into broader retinoid metabolism and signaling pathways that govern cellular functions.

Dysregulation of RBP1 can contribute to conditions such as age-related macular degeneration (AMD) and metabolic syndrome. In AMD impaired retinol transport and metabolism lead to retinal damage and vision loss. In metabolic syndrome altered retinoid homeostasis involving RBP1 may exacerbate insulin resistance and obesity. Relationships with proteins like LRAT and retinaldehyde dehydrogenase (RALDH) highlight its involvement in disease mechanisms related to retinol dysfunction.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cytoplasmic retinol-binding protein (PubMed : 22665496, PubMed : 26900151, PubMed : 28057518). Accepts retinol from the transport protein STRA6, and thereby contributes to retinol uptake, storage and retinoid homeostasis (PubMed : 15632377, PubMed : 22665496).

See full target information RBP1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 21: PubMed32422974

2020

Proteomic Characterization of Colorectal Cancer Cells versus Normal-Derived Colon Mucosa Cells: Approaching Identification of Novel Diagnostic Protein Biomarkers in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Maja Ludvigsen,Louise Thorlacius-Ussing,Henrik Vorum,Mary Pat Moyer,Mogens Tornby Stender,Ole Thorlacius-Ussing,Bent Honoré

Proteomics 10:3480-93 PubMed20815088

2010

Proteomic analysis of testis biopsies in men treated with transient scrotal hyperthermia reveals the potential targets for contraceptive development.

Applications

Species

Human

Hui Zhu,Yugui Cui,Jin Xie,Ling Chen,Xiangxiang Chen,Xuejiang Guo,Yefei Zhu,Xinghai Wang,Jiansun Tong,Zuomin Zhou,Yue Jia,Yan-He Lue,Amiya Sinha Hikim,Christina Wang,Ronald S Swerdloff,Jiahao Sha

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RBP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RBP1 antibody (AB31106)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise