JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB101457

Anti-RDH5 antibody

Be the first to review this product! 리뷰 제출

|

(2 제품이 사용된 논문 )

Rabbit Polyclonal RDH5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RDH5 aa 100-250.

대체 명칭 보기

HSD17B9, RDH1, SDR9C5, RDH5, Retinol dehydrogenase 5, 11-cis retinol dehydrogenase, 9-cis retinol dehydrogenase, Short chain dehydrogenase/reductase family 9C member 5, 11-cis RDH, 11-cis RoDH, 9cRDH

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RDH5 antibody (AB101457)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RDH5 antibody (AB101457)

Immunohistochemical analysis of RDH5 in paraffin-embedded Cal27 xenograft, using ab101457 at 1/100 dilution.

Western blot - Anti-RDH5 antibody (AB101457)
  • WB

Unknown

Western blot - Anti-RDH5 antibody (AB101457)

10% SDS-PAGE

All lanes:

Western blot - Anti-RDH5 antibody (ab101457) at 1/1000 dilution

All lanes:

NT2D1 whole cell lysate at 30 µg

Predicted band size: 35 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RDH5 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q92781

style
left-column

대체품

Primary Antibodies

AB200197

Anti-RDH5 antibody [EPR15191]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RDH5 antibody [EPR15191] (AB200197)

  • 0
  • 0

보완 제품

Primary Antibodies

AB137304

Anti-LRAT antibody

  • 0
  • 1
Primary Antibodies

AB74073

Anti-TRP2/DCT antibody

Western blot - Anti-TRP2/DCT antibody (AB74073)

  • 5
  • 9
Primary Antibodies

AB231782

Anti-RPE65 antibody [EPR22579-44]

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPE65 antibody [EPR22579-44] (AB231782)

  • 0
  • 0
Primary Antibodies

AB77285

Anti-ABCA4 antibody [3F4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA4 antibody [3F4] (AB77285)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Use 10mM Citrate buffer or Tris-EDTA buffer (pH8.0)</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RDH5 also known as retinol dehydrogenase 5 or 11-cis retinol dehydrogenase is an enzyme that plays an important role in the visual cycle. It has a molecular mass of approximately 35 kDa and primarily functions in the conversion of 11-cis-retinol to 11-cis-retinal which is a significant step in the biosynthesis of visual pigments. RDH5 is expressed mainly in the retinal pigment epithelium of the eye where it contributes to maintaining proper vision.
Biological function summary

11-cis retinol dehydrogenase facilitates the regeneration of 11-cis-retinal from 11-cis-retinol a critical process for photoreceptor cell function and visual cycle continuity. RDH5 operates as part of a complex of enzymes involved in the retinoid cycle which is essential for maintaining a continuous supply of 11-cis-retinal. Without RDH5 the regeneration of 11-cis-retinal would be ineffective reducing the ability to see especially in low-light conditions.

Pathways

The retinol dehydrogenase 5 enzyme plays a major role in the visual cycle pathway. This pathway is necessary for converting retinoids into visual chromophores that allow vision. RDH5 interacts closely with other key enzymes such as RPE65 and RDH12 which also participate in retinoid transformations. The proper functioning of the visual cycle pathway depends on the actions of these enzymes to ensure the availability of 11-cis-retinal for photoreceptor function.

Defects in the RDH5 gene can lead to retinal degenerative diseases like fundus albipunctatus and retinitis pigmentosa. These conditions are characterized by night blindness and progressive vision loss. Mutations in RDH5 affect the synthesis of 11-cis-retinal disrupting the visual cycle and contributing to these diseases. RDH5 shares a pathway with RPE65 another protein implicated in retinal disorders highlighting the interconnectedness of proteins involved in the visual cycle and their impact on eye health.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Catalyzes the oxidation of cis-isomers of retinol, including 11-cis-, 9-cis-, and 13-cis-retinol in an NAD-dependent manner (PubMed : 10588954, PubMed : 11675386, PubMed : 9115228, PubMed : 9931293). Has no activity towards all-trans retinal (By similarity). Plays a significant role in 11-cis retinol oxidation in the retinal pigment epithelium cells (RPE). Also recognizes steroids (androsterone, androstanediol) as its substrates (PubMed : 29541409, PubMed : 9931293).
See full target information RDH5
style
left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Investigative ophthalmology & visual science 61:9 PubMed32049341

2020

Increased Rate of Retinal Pigment Epithelial Cell Migration and Pro-Angiogenic Potential Ensuing From Reduced Cystatin C Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Emil Carlsson,Wasu Supharattanasitthi,Malcolm Jackson,Luminita Paraoan

Molecular vision 23:60-89 PubMed28356702

2017

Appropriately differentiated ARPE-19 cells regain phenotype and gene expression profiles similar to those of native RPE cells.

Applications

Unspecified application

Species

Human

William Samuel,Cynthia Jaworski,Olga A Postnikova,R Krishnan Kutty,Todd Duncan,Li Xuan Tan,Eugenia Poliakov,Aparna Lakkaraju,T Michael Redmond
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com