AB238162

Anti-RECK antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal RECK antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RECK aa 1-250.

대체 명칭 보기

ST15, RECK, Reversion-inducing cysteine-rich protein with Kazal motifs, hRECK, Suppressor of tumorigenicity 15 protein

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECK antibody (AB238162)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECK antibody (AB238162)

Paraffin-embedded human liver tissue stained for RECK using ab238162 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECK antibody (AB238162)

Paraffin-embedded human kidney tissue stained for RECK using ab238162 at 1/300 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RECK antibody (AB238162)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RECK antibody (AB238162)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for RECK (green) using ab238162 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RECK aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

O95980

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RECK also known as Reversion-inducing-cysteine-rich protein with kazal motifs is a 110 kDa membrane-anchored glycoprotein. It plays a role in inhibiting matrix metalloproteinases (MMPs) like MMP-2 and MMP-9 which are involved in extracellular matrix remodeling. RECK is widely expressed in various tissues including the brain heart and muscles. It interacts with other membrane proteins to modulate different cellular functions playing a part in maintaining the structure of tissues.

Biological function summary

RECK supports angiogenesis and tissue integrity by controlling matrix degradation. It is essential for proper vascular development. Although RECK is not part of a bigger complex it exerts its function by partnering with integrins and other membrane components. This interaction helps regulate cell migration and adhesion which are significant for normal development and wound healing processes.

Pathways

RECK engages in the regulation of the extracellular matrix and signaling cascades. It aligns with critical pathways like the Wnt and Rho GTPase pathways. These pathways are important in controlling cellular proliferation and movement. RECK's interaction with proteins like β-catenin and Ras further illustrates its importance in modulating cellular pathways connected to growth and movement.

RECK can affect cancer progression and metastasis. Its downregulation or malfunction is associated with increased tumor invasion and angiogenesis due to uncontrolled MMP activity. RECK's connection with MMP-2 and MMP-9 is significant here as these proteins promote tumor metastasis. Its role is also evident in neurological disorders like cerebral ischemia where defective matrix remodeling can exacerbate tissue damage. Researchers focus on RECK to develop strategies for cancer therapeutics and neuroprotective treatments.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Functions together with ADGRA2 to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed : 28289266, PubMed : 30026314). Plays a key role in Wnt7-specific responses : required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (By similarity). Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands : acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7 (PubMed : 30026314). ADGRA2 is then required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (PubMed : 30026314). Also acts as a serine protease inhibitor : negatively regulates matrix metalloproteinase-9 (MMP9) by suppressing MMP9 secretion and by direct inhibition of its enzymatic activity (PubMed : 18194466, PubMed : 9789069). Also inhibits metalloproteinase activity of MMP2 and MMP14 (MT1-MMP) (PubMed : 9789069).

See full target information RECK

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cancer research and clinical oncology 150:294 PubMed38842683

2024

LncRNA HOXC-AS3 accelerates malignant proliferation of cervical cancer cells via stabilizing KDM5B.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Fang Hou,Zhenghua Teng,Weiwei Xia,Jie Peng

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 41:2546-2560 PubMed33818185

2021

A large portion of the astrocyte proteome is dedicated to perivascular endfeet, including critical components of the electron transport chain.

Applications

Unspecified application

Species

Unspecified reactive species

Jesse A Stokum,Bosung Shim,Weiliang Huang,Maureen Kane,Jesse A Smith,Volodymyr Gerzanich,J Marc Simard

Oncology letters 21:256 PubMed33664820

2021

lncRNA XIST regulates cell proliferation, migration and invasion via regulating miR-30b and RECK in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Sun,Min Zhang,Hongxia Qu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RECK antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECK antibody (AB238162)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECK antibody (AB238162)

Immunocytochemistry/ Immunofluorescence - Anti-RECK antibody (AB238162)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise