JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB323859

Anti-Ref2P antibody [EPR28159-511]

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Rabbit Recombinant Monoclonal REF2P antibody. Suitable for WB and reacts with Drosophila melanogaster samples.
2 이미지
Western blot - Anti-Ref2P antibody [EPR28159-511] (AB323859)
  • WB

Supplier Data

Western blot - Anti-Ref2P antibody [EPR28159-511] (AB323859)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of Ref2P is upregulated in response to reducing Atg8a (PMID : 18347073).

Loading control : Anti-Actin antibody [EPR16769] (ab179467) 1 : 5000 (42Kda).

All lanes:

Western blot - Anti-Ref2P antibody [EPR28159-511] (ab323859) at 1/1000 dilution

Lane 1:

SL2 (Drosophila melanogaster embryo epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

SL2 transfected with siRNA specifically targeting Atg8a whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa,42 kDa

false

Exposure time: 180s

Western blot - Anti-Ref2P antibody [EPR28159-511] (AB323859)
  • WB

Supplier Data

Western blot - Anti-Ref2P antibody [EPR28159-511] (AB323859)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Loading control : Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) 1 : 100000 (15Kda).

All lanes:

Western blot - Anti-Ref2P antibody [EPR28159-511] (ab323859) at 1/1000 dilution

Lane 1:

SL2 (Drosophila melanogaster embryo epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

SL2 transfected with siRNA specifically targeting Ref2P whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa,15 kDa

false

Exposure time: 180s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28159-511

Isotype

IgG

Carrier free

No

Reacts with

Drosophila melanogaster

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

보완 제품

Primary Antibodies

AB109364

Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB109364)

  • 4
  • 12
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB30687

Anti-LAMP1 antibody - Lysosome Marker

Lab Essentials

Immunocytochemistry - Anti-LAMP1 antibody - Lysosome Marker (AB30687)

  • 4
  • 4
Primary Antibodies

AB14748

Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (AB14748)

  • 5
  • 27
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Drosophila melanogaster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Required for selective autophagy activation by ubiquitinated proteins (PubMed : 22622177). Implicated in sigma rhabdovirus multiplication and necessary for male fertility. Involved in activating transcription of Drs (PubMed : 12446795, PubMed : 2510997).
See full target information ref(2)P

대체 명칭 보기

CG10360, ref(2)P, Protein ref(2)P, Refractory to sigma P
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com