Anti-Renin antibody [EPR20693]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (AB212197)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Renin with ab212197 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on juxtaglomerular cells of human kidney (PMID : 1898348). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Renin antibody [EPR20693] (AB212197)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1/2000 dilution, ab273021 anti-V2R used at 1/100 dilution and ab212197 anti-Renin used at a 1/4000 dilution.

Panel A : merged staining of anti-GLEPP1/PTPRO (green; Opal™520), anti-V2R (magenta; Opal™690) and anti-Renin (gray; Opal™570) on human kidney.

Panel B : anti-GLEPP1/PTPRO staining glomerulus in human kidney.

Panel C : anti-V2R staining connecting ducts in human kidney.

Panel D : anti-Renin staining juxtaglomerular cells in human kidney.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322047, ab273021 and ab212197 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Renin antibody [EPR20693] (AB212197)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining CD34 with ab317588 at a 1/2000 dilution, ab199975 anti-Aquaporin 2 used at 1/4000 dilution and ab212197 anti-Renin used at a 1/4000 dilution.

Panel A : merged staining of anti-CD34 (green; Opal™520), anti-Aquaporin 2 (magenta; Opal™690) and anti-Renin (gray; Opal™570) on human kidney.

Panel B : anti-CD34 staining endothelium in human kidney.

Panel C : anti-Aquaporin 2 staining collecting tubules in human kidney.

Panel D : anti-Renin staining juxtaglomerular cells in human kidney.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317588, ab199975 and ab212197 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Renin antibody [EPR20693] (AB212197)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining CD34 with ab317588 at a 1/2000 dilution, ab321787 anti-SGLT1 used at 1/2000 dilution and ab212197 anti-Renin used at a 1/4000 dilution.

Panel A : merged staining of anti-CD34 (green; Opal™520), anti-SGLT1 (magenta; Opal™690) and anti-Renin (gray; Opal™570) on human kidney.

Panel B : anti-CD34 staining endothelium in human kidney.

Panel C : anti-SGLT1 staining tubules in human kidney.

Panel D : anti-Renin staining juxtaglomerular cells in human kidney.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317588, ab321787 and ab212197 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Renin antibody [EPR20693] (AB212197)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining SLC6A19 with ab326561 at a 1 : 500 (0.996 µg/ml) dilution, ab322047 anti-GLEPP1/PTPRO used at 1 : 2000 (0.261 µg/ml) dilution and ab212197 anti-REN used at a 1 : 8000 (0.133 µg/ml) dilution.

Panel A : anti-SLC6A19 (green; Opal™520), anti-GLEPP1/PTPRO (magenta; Opal™690), anti-REN (gray; Opal™570) on human kidney.
Panel B : anti-SLC6A19 staining apical membrane of kidney tubules in human kidney.
Panel C : anti-GLEPP1/PTPRO staining glomerulus in human kidney.
Panel D : anti-REN staining juxtaglomerular cells in human kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab326561, ab322047 and ab212197 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Renin antibody [EPR20693] (AB212197)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1/2000 dilution, ab199975 anti-Aquaporin 2 used at 1/4000 dilution and ab212197 anti-Renin used at a 1/4000 dilution.

Panel A : merged staining of anti-GLEPP1/PTPRO (green; Opal™520), anti-Aquaporin 2 (magenta; Opal™690) and anti-Renin (gray; Opal™570) on human kidney.

Panel B : anti-GLEPP1/PTPRO staining glomerulus in human kidney.

Panel C : anti-Aquaporin 2 staining collecting tubules in human kidney.

Panel D : anti-Renin staining juxtaglomerular cells in human kidney.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322047, ab199975 and ab212197 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Renin antibody [EPR20693] (AB212197)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse kidney tissue staining SLC6A19 with ab326561 at a 1 : 500 (0.996 µg/ml) dilution, ab322047 anti-GLEPP1/PTPRO used at 1 : 2000 (0.261 µg/ml) dilution and ab212197 anti-REN used at a 1 : 8000 (0.133 µg/ml) dilution.

Panel A : anti-SLC6A19 (green; Opal™520), anti-GLEPP1/PTPRO (magenta; Opal™690), anti-REN (gray; Opal™570) on mouse kidney.
Panel B : anti-SLC6A19 staining apical membrane of kidney tubules in mouse kidney.
Panel C : anti-GLEPP1/PTPRO staining glomerulus in mouse kidney.
Panel D : anti-REN staining juxtaglomerular cells in mouse kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab326561, ab322047 and ab212197 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC

Lab

Immunohistochemistry - Anti-Renin antibody [EPR20693] (AB212197)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse kidney tissue staining PAR1/Thrombin Receptor with ab306558 at a 1/500 dilution, ab306558 anti-SGLT2 used at 1/5000 dilution and ab212197 anti-Renin used at a 1/4000 dilution.

Panel A : merged staining of anti-PAR1/Thrombin Receptor (green; Opal™520), anti-SGLT2 (magenta; Opal™690) and anti-Renin (gray; Opal™570) on mouse kidney.

Panel B : anti-PAR1/Thrombin Receptor staining endothelium in mouse kidney.

Panel C : anti-SGLT2 staining the brush border of proximal tubules in mouse kidney.

Panel D : anti-Renin staining juxtaglomerular cells in mouse kidney.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322457, ab306558 and ab212197 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (AB212197)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Renin with ab212197 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on juxtaglomerular cells of mouse kidney (PMID : 1898348). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (AB212197)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Renin with ab212197 at 1/6000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in juxtaglomerular cells of rat kidney tissue section (PMID : 27080979).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Heat mediated antigen retrieval performed using Tris-EDTA buffer (pH9.0) (ab94681).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Renin antibody [EPR20693] (AB212197)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Renin with ab212197 at 1/6000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in juxtaglomerular cells of mouse kidney tissue section (PMID : 27080979).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Heat mediated antigen retrieval performed using Tris-EDTA buffer (pH9.0) (ab94681).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Renin antibody [EPR20693] (AB212197)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1/2000 dilution, ab199975 anti-Aquaporin 2 used at 1/4000 dilution and ab212197 anti-Renin used at a 1/4000 dilution.

Panel A : merged staining of anti-GLEPP1/PTPRO (green; Opal™520), anti-Aquaporin 2 (magenta; Opal™690) and anti-Renin (gray; Opal™570) on human kidney.

Panel B : anti-GLEPP1/PTPRO staining glomerulus in human kidney.

Panel C : anti-Aquaporin 2 staining collecting tubules in human kidney.

Panel D : anti-Renin staining juxtaglomerular cells in human kidney.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322047, ab199975 and ab212197 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Renin antibody [EPR20693] (AB212197)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse kidney tissue staining CD34 with ab317588 at a 1/5000 dilution, ab199975 anti-Aquaporin 2 used at 1/4000 dilution and ab212197 anti-Renin used at a 1/4000 dilution.

Panel A : merged staining of anti-CD34 (green; Opal™520), anti-Aquaporin 2 (magenta; Opal™690) and anti-Renin (gray; Opal™570) on human kidney.

Panel B : anti-CD34 staining endothelium in mouse kidney.

Panel C : anti-Aquaporin 2 staining collecting tubules in mouse kidney.

Panel D : anti-Renin staining juxtaglomerular cells in mouse kidney.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317588, ab199975 and ab212197 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.