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AB204178

Anti-Reticulocalbin 3 antibody

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(6 제품이 사용된 논문 )

Rabbit Polyclonal Reticulocalbin 3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RCN3 aa 150-300.

대체 명칭 보기

UNQ239/PRO272, RCN3, Reticulocalbin-3, EF-hand calcium-binding protein RLP49

7 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (AB204178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (AB204178)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling Reticulocalbin 3 with ab204178 at 1/2500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (AB204178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (AB204178)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Reticulocalbin 3 with ab204178 at 1/2500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (AB204178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (AB204178)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling Reticulocalbin 3 with ab204178 at 1/2500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (AB204178)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulocalbin 3 antibody (AB204178)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human upper gastrointestinal tract tissue labelling Reticulocalbin 3 with ab204178 at 1/2500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Reticulocalbin 3 antibody (AB204178)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Reticulocalbin 3 antibody (AB204178)

Immunofluorescent analysis of PFA-fixed,Triton X-100 permeabilized U-2 OS cells labeling Reticulocalbin 3 with ab204178 at 4 μg/ml (green).

Western blot - Anti-Reticulocalbin 3 antibody (AB204178)
  • WB

Supplier Data

Western blot - Anti-Reticulocalbin 3 antibody (AB204178)

All lanes:

Western blot - Anti-Reticulocalbin 3 antibody (ab204178) at 0.4 µg/mL

All lanes:

AN3-CA (human endometrium adenocarcinoma cell line) cell lysate

Predicted band size: 37 kDa

false

Western blot - Anti-Reticulocalbin 3 antibody (AB204178)
  • WB

CiteAb

Western blot - Anti-Reticulocalbin 3 antibody (AB204178)

Reticulocalbin 3 western blot using anti-Reticulocalbin 3 antibody ab204178. Publication image and figure legend from Cai, R., Wang, P., et al., 2020, J Transl Med, PubMed 32216815.

ab204178 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab204178 please see the product overview.

Identification and verification of DEPs in ESCC tissues. a Paired ESCC and adjacent normal esophageal tissues from three patients were analyzed by TMT. Twofold change was defined as the threshold for a significant change in expression. We detected 39 proteins up-regulated in cancer tissues compared with that in adjacent normal tissues (paired-samples t-test, p < 0.001). b Gene ontology enrichment analysis of identified DEPs which were classified into cellular component, molecular function, and biological process. c, d Validation of DEPs in ESCA. The expression of FN1, LTBP1, PLOD2, THBS1 and RCN3 were confirmed by GEPIA and western blot analysis. N non-tumorous tissues, T tumorous tissues. (one-way ANOVA and paired-samples t-test, *p < 0.05)

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RCN3 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q96D15

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Reticulocalbin 3 also known as ERC-55 or RCN3 is a calcium-binding protein localized in the endoplasmic reticulum. It has a molecular mass of around 44 kDa. Reticulocalbin 3 is expressed in various tissues with strong expression in the heart liver and skeletal muscles. It binds calcium through its EF-hand domains which are responsible for its ability to interact with other calcium-dependent proteins and ions. This makes it an important component of maintaining calcium homeostasis within the endoplasmic reticulum.
Biological function summary

Reticulocalbin 3 plays a role in calcium-dependent signaling processes that affect protein folding and secretion. It is part of a complex involved in glycoprotein maturation and quality control within the endoplasmic reticulum. Reticulocalbin 3 helps ensure proper protein folding which is essential for cellular function. By influencing endoplasmic reticulum stress responses Reticulocalbin 3 contributes to overall cellular health and functionality.

Pathways

The role of Reticulocalbin 3 can be seen in the endoplasmic reticulum-associated degradation (ERAD) pathway and the unfolded protein response (UPR). These pathways help manage protein misfolding and cellular stress levels. Within these pathways Reticulocalbin 3 interacts with proteins like calreticulin and calnexin which are also involved in calcium binding and protein folding mechanisms. Its function supports the regulation and maintenance of protein quality control aiding in the survival of stressed cells.

Reticulocalbin 3 shows a connection with diseases involving improper protein folding such as cystic fibrosis and some forms of liver disease. For instance in cystic fibrosis defective protein folding affects the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Reticulocalbin 3 may interact with proteins such as BiP/GRP78 involved in maintaining proper protein conformation. By stabilizing protein folding pathways Reticulocalbin 3 potentially influences the progression and severity of these conditions making it an interesting target for therapeutic intervention.

제품 프로토콜

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타겟 정보

Probable molecular chaperone assisting protein biosynthesis and transport in the endoplasmic reticulum (PubMed : 16433634, PubMed : 28939891). Required for the proper biosynthesis and transport of pulmonary surfactant-associated protein A/SP-A, pulmonary surfactant-associated protein D/SP-D and the lipid transporter ABCA3 (By similarity). By regulating both the proper expression and the degradation through the endoplasmic reticulum-associated protein degradation pathway of these proteins plays a crucial role in pulmonary surfactant homeostasis (By similarity). Has an anti-fibrotic activity by negatively regulating the secretion of type I and type III collagens (PubMed : 28939891). This calcium-binding protein also transiently associates with immature PCSK6 and regulates its secretion (PubMed : 16433634).
See full target information RCN3

제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 15:878 PubMed39632825

2024

Colorectal cancer patients-derived immunity-organoid platform unveils cancer-specific tissue markers associated with immunotherapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

A Esposito,A Agostini,G Quero,G Piro,L Priori,A Caggiano,G Scaglione,A Battaglia,M A Calegari,L Salvatore,M Bensi,M G Maratta,A Ceccarelli,G Trovato,G Genovese,E Gurreri,S Ascrizzi,M Martini,C Fiorillo,A Fattorossi,F De Sanctis,S Ugel,V Corbo,S Alfieri,G Tortora,C Carbone

Cancers 15: PubMed37046668

2023

Reticulocalbin 3 Is a Novel Mediator of Glioblastoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yi He,Salvador Alejo,Jessica D Johnson,Sridharan Jayamohan,Gangadhara R Sareddy

Cancer science 113:3593-3607 PubMed35839283

2022

Reticulocalbin3: A Ca homeostasis regulator that promotes esophageal squamous cell carcinoma progression and cisplatin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Cai,Ping Wang,Xin Zhao,Xiansheng Lu,Ruxia Deng,Xiaoyu Wang,Chang Hong,Jie Lin

Scientific reports 11:10868 PubMed34035379

2021

Reticulocalbin 3 is involved in postnatal tendon development by regulating collagen fibrillogenesis and cellular maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Na Rae Park,Snehal S Shetye,Igor Bogush,Douglas R Keene,Sara Tufa,David M Hudson,Marilyn Archer,Ling Qin,Louis J Soslowsky,Nathaniel A Dyment,Kyu Sang Joeng

EMBO molecular medicine 13:e13074 PubMed33998164

2021

Impairment of the ER/mitochondria compartment in human cardiomyocytes with PLN p.Arg14del mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Friederike Cuello,Anika E Knaust,Umber Saleem,Malte Loos,Janice Raabe,Diogo Mosqueira,Sandra Laufer,Michaela Schweizer,Petra van der Kraak,Frederik Flenner,Bärbel M Ulmer,Ingke Braren,Xiaoke Yin,Konstantinos Theofilatos,Jorge Ruiz-Orera,Giannino Patone,Birgit Klampe,Thomas Schulze,Angelika Piasecki,Yigal Pinto,Aryan Vink,Norbert Hübner,Sian Harding,Manuel Mayr,Chris Denning,Thomas Eschenhagen,Arne Hansen

Journal of translational medicine 18:139 PubMed32216815

2020

LTBP1 promotes esophageal squamous cell carcinoma progression through epithelial-mesenchymal transition and cancer-associated fibroblasts transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Cai,Ping Wang,Xin Zhao,Xiansheng Lu,Ruxia Deng,Xiaoyu Wang,Zhaoji Su,Chang Hong,Jie Lin
제품이 사용된 논문 모두 보기

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