AB250585

Anti-RFC3 antibody [EPR14214] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(1 출판물)

Rabbit Recombinant Monoclonal RFC3 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

Replication factor C subunit 3, Activator 1 38 kDa subunit, Activator 1 subunit 3, Replication factor C 38 kDa subunit, A1 38 kDa subunit, RF-C 38 kDa subunit, RFC38, RFC3

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-RFC3 antibody [EPR14214] - BSA and Azide free (AB250585)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RFC3 antibody [EPR14214] - BSA and Azide free (AB250585)

This data was developed using ab182143, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling RFC3 using ab182143 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

Flow Cytometry (Intracellular) - Anti-RFC3 antibody [EPR14214] - BSA and Azide free (AB250585)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RFC3 antibody [EPR14214] - BSA and Azide free (AB250585)

This data was developed using ab182143, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa cells labeling RFC3 using ab182143 at a 1/250 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde

Supplier Data

Western blot - Anti-RFC3 antibody [EPR14214] - BSA and Azide free (AB250585)

This data was developed using ab182143, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RFC3 antibody [EPR14214] (<a href='/ko/products/primary-antibodies/rfc3-antibody-epr14214-ab182143'>ab182143</a>) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 41 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14214

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

제품 세부 정보

ab250585 is the carrier-free version of ab182143.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Replication Factor C subunit 3 (RFC3) also known as RFC38 or RFC37 plays an important role in DNA replication and repair by forming part of the replication factor C complex. RFC3 is a protein with a molecular mass of approximately 38 kDa. It is ubiquitously expressed in various tissues where it contributes to maintaining genomic integrity.

Biological function summary

RFC3 facilitates the loading of the proliferating cell nuclear antigen (PCNA) onto DNA during the S phase of the cell cycle. As a component of the replication factor C (RFC) complex which is essential for DNA replication RFC3 works with other subunits to ensure the accurate duplication of the genome. It is important for the coordination and smooth progress of DNA synthesis which is vital for cell division and growth.

Pathways

RFC3 holds a significant role in the DNA replication and cell cycle pathways. By interacting with proteins like PCNA RFC3 helps initiate and regulate the proper progression through the cell cycle checkpoints. The RFC complex which includes RFC3 is involved in tethering DNA polymerases to template DNA facilitating continuous DNA synthesis. This important interaction ensures the fidelity and efficiency of the DNA replication process.

Alterations in RFC3 expression or function can relate to cancer and genomic instability disorders. Elevated levels of RFC3 have been observed in various types of cancer where it often links with other overexpressed proteins like PCNA contributing to uncontrolled cell proliferation. Investigating RFC3's role and its interactions with related proteins helps in understanding tumorigenesis and developing potential therapeutic strategies.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA.

See full target information Replication factor C subunit 3

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology letters 26:458 PubMed37736555

2023

S100A6 is a potential diagnostic and prognostic biomarker for human glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hong,Hui Zhang,Yufei Xiao,Lingwei Shen,Yun Qian

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RFC3 antibody [EPR14214] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-RFC3 antibody [EPR14214] - BSA and Azide free (AB250585)

Flow Cytometry (Intracellular) - Anti-RFC3 antibody [EPR14214] - BSA and Azide free (AB250585)

Western blot - Anti-RFC3 antibody [EPR14214] - BSA and Azide free (AB250585)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise