AB168475

Anti-RFX3 antibody

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Mouse Polyclonal RFX3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RFX3.

대체 명칭 보기

Transcription factor RFX3, Regulatory factor X 3, RFX3

1 이미지

Immunocytochemistry/ Immunofluorescence - Anti-RFX3 antibody (AB168475)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RFX3 antibody (AB168475)

Immunofluorescent analysis of HeLa cells labeling RFX3 with ab168475 at 10 µg/ml.

주요 정보

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RFX3.

P48380

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The RFX3 protein also known as Regulatory Factor X 3 plays a significant role in gene transcription regulation. It has a molecular mass of approximately 83 kDa. RFX3 functions as a transcription factor binding specifically to X-box motifs within DNA influencing the expression of various genes important for cell processes. This protein is present in a variety of tissues including the brain kidney and pancreas suggesting its diverse and widespread role in cellular activities.

Biological function summary

The function of RFX3 involves the modulation of genes critical for cilia development and function. RFX3 forms part of a gene regulatory complex that oversees ciliogenesis. Through this complex it controls the transcription of genes essential for the formation of motile and primary cilia. The activity of RFX3 maintains proper cilia structure and movement essential for cellular signaling and environmental sensing.

Pathways

The involvement of RFX3 focuses largely on the ciliogenesis and hedgehog signaling pathways. RFX3 regulates genes necessary for the assembly and function of cilia linking it to the hedgehog signaling pathway where cilia play an important role in signal reception and transduction. RFX3 interacts with proteins like RFX1 and RFX2 which share similar DNA-binding domains highlighting its preserved function across these pathways.

Disruptions in RFX3 can relate to ciliopathies and diabetes. Ciliopathies such as Joubert syndrome arise from defective ciliogenesis due to compromised RFX3 function with related proteins like RFX1 also being implicated. Diabetes connects to RFX3 through its expression in pancreatic beta cells suggesting a role in insulin regulation and glucose metabolism. Changes in RFX3 levels or function can affect genes involved in these conditions indicating its importance in maintaining normal physiological processes.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5) (By similarity). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes (PubMed : 20148032). Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells (PubMed : 12411430).

See full target information RFX3

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

OncoTargets and therapy 12:3743-3751 PubMed31190875

2019

Homeobox B7 accelerates the cancer progression of gastric carcinoma cells by promoting epithelial-mesenchymal transition (EMT) and activating Src-FAK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianghong Wu,Ziwen Long,Hong Cai,Shengjia Yu,Xiaowen Liu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RFX3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RFX3 antibody (AB168475)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise