AB236408

Anti-RFXANK antibody [OTI3E7]

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Mouse Monoclonal RFXANK antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate - Human, Transfected cell line - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RFXANK.

대체 명칭 보기

ANKRA1, RFXB, RFXANK, DNA-binding protein RFXANK, Ankyrin repeat family A protein 1, Regulatory factor X subunit B, Regulatory factor X-associated ankyrin-containing protein, RFX-B

10 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Paraffin-embedded human bladder carcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Paraffin-embedded human tonsil tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Paraffin-embedded human kidney carcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Paraffin-embedded human ovary adenocarcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Paraffin-embedded human pancreas tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Paraffin-embedded human kidney tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling RFXANK with ab226408 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling RFXANK with ab226408 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RFXANK antibody [OTI3E7] (AB236408)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RFXANK antibody [OTI3E7] (AB236408)

COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY RFXANK stained for RFXANK (green) using ab236408 at 1/100 dilution in ICC/IF.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI3E7

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RFXANK.

O14593

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" }, "Transfected cell line - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Clone OTI3E7 (formerly 3E7).

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

Purified from cell culture supernatant.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The RFXANK protein also known as regulatory factor X-associated ankyrin-containing protein is a component of the RFX complex. RFXANK has a molecular mass of approximately 27 kDa. This protein plays a mechanical role in the regulation of transcription by interacting with DNA sequences and recruiting various co-factors necessary for gene expression. RFXANK is largely expressed in immune cells where it modulates processes important for immune function.

Biological function summary

RFXANK acts as part of the RFX complex which is known for its role in the regulation of class II major histocompatibility complex (MHC) genes. These genes are significant for proper immune response allowing immune cells to present antigens and initiate defense mechanisms. RFXANK through its interaction with other proteins like RFX5 and RFXAP stabilizes the DNA binding ability of the RFX complex enabling the transcription of genes essential for antigen presentation.

Pathways

The regulatory influence of RFXANK integrates into important immune pathways such as the MHC class II antigen presentation pathway. RFXANK's partnership with RFX5 and RFXAP facilitates binding to the X-box motif of MHC class II promoters ensuring the transcription initiation required for effective immune surveillance. These relations evidence RFXANK's vital place in immune system operations particularly in adaptive immunity.

RFXANK mutations or dysregulation relate to bare lymphocyte syndrome type II a severe immunodeficiency disorder. This condition arises when RFXANK fails to properly regulate MHC class II expression compromising antigen presentation and adaptive immune response. Connections exist between RFXANK and other disease-related proteins like RFX5 which highlight disruptions at the molecular level impacting immune functionality.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters (PubMed : 10072068, PubMed : 10725724, PubMed : 9806546). May also potentiate the activation of RAF1 (By similarity).. Isoform 2 is not involved in the positive regulation of MHC class II genes.

See full target information RFXANK

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of digestive diseases 23:376-387 PubMed35844201

2022

Circular RNA circ_0006089 promotes the progression of gastric cancer by regulating the miR-143-3p/PTBP3 axis and PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guang Rong Lin,Wei Rong Chen,Pei Hong Zheng,Wei Shan Chen,Gao Yang Cai

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RFXANK antibody [OTI3E7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Flow Cytometry (Intracellular) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Flow Cytometry (Intracellular) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFXANK antibody [OTI3E7] (AB236408)

Immunocytochemistry/ Immunofluorescence - Anti-RFXANK antibody [OTI3E7] (AB236408)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise