AB150408

Anti-RHOBTB1 antibody [EPR8990]

RabMAb
Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(1 출판물)

Rabbit Recombinant Monoclonal RHOBTB1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-RHOBTB1 antibody [EPR8990] (AB150408)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RHOBTB1 antibody [EPR8990] (AB150408)

Immunofluorescent analysis of JAR cells labelling RHOBTB1 with ab150408 at a dilution of 1/250.

Unknown

Western blot - Anti-RHOBTB1 antibody [EPR8990] (AB150408)

All lanes:

Western blot - Anti-RHOBTB1 antibody [EPR8990] (ab150408) at 1/1000 dilution

Lane 1:

Human stomach lysates at 10 µg

Lane 2:

Human fetal kidney lysates at 10 µg

Lane 3:

Human placenta lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 79 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8990

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" } } }

style

left-column

제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RHOBTB1 also known as Rho-related BTB domain-containing protein 1 is a member of the Rho GTPase family. It has a molecular mass of approximately 82 kDa. This protein is mainly expressed in the brain and testis with lower levels found in other tissues. RHOBTB1 functions by acting as a molecular switch that cycles between an active GTP-bound state and an inactive GDP-bound state which regulates various cellular processes.

Biological function summary

RHOBTB1 is involved in the regulation of cytoskeletal dynamics which affects cell shape motility and possibly cell division. It is also implicated in intracellular signaling processes that control cell morphology. RHOBTB1 does not function alone; it interacts with other proteins possibly forming part of larger protein complexes that further expand its role in cellular activities.

Pathways

RHOBTB1 plays a role in signaling pathways that include the Rho GTPase cycle and cellular processes related to cytoskeletal reorganization. In these pathways RHOBTB1 interacts with proteins such as CDC42 and RAC1 which are essential for actin filament polymerization and cellular movement. These interactions help facilitate normal cellular dynamics and responses to extracellular signals.

Mutations or dysregulation of RHOBTB1 have been linked to neurological conditions and certain cancers. For example RHOBTB1 abnormalities have been associated with an increased risk of glioblastoma where it might interact with tumor suppressor proteins like p53. Additionally RHOBTB1's role in vacuole formation has implications in lysosomal storage diseases connecting it to other lysosomal proteins involved in these processes.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

See full target information RHOBTB1

대체 명칭 보기

KIAA0740, RHOBTB1, Rho-related BTB domain-containing protein 1

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Acta neuropathologica 147:65 PubMed38557897

2024

Landscape of brain myeloid cell transcriptome along the spatiotemporal progression of Alzheimer's disease reveals distinct sequential responses to Aβ and tau.

Applications

Unspecified application

Species

Unspecified reactive species

Astrid Wachter,Maya E Woodbury,Sylvia Lombardo,Aicha Abdourahman,Carolin Wuest,Emily McGlame,Timothy Pastika,Joseph Tamm,Nandini Romanul,Kiran Yanamandra,Rachel Bennett,Gen Lin,Taekyung Kwon,Fan Liao,Corinna Klein,Yelena Grinberg,Methasit Jaisa-Aad,Huan Li,Matthew P Frosch,Markus P Kummer,Sudeshna Das,Tammy Dellovade,Eric H Karran,Xavier Langlois,Janina S Ried,Alberto Serrano-Pozo,Robert V Talanian,Knut Biber,Bradley T Hyman

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RHOBTB1 antibody [EPR8990]

Immunocytochemistry/ Immunofluorescence - Anti-RHOBTB1 antibody [EPR8990] (AB150408)

Western blot - Anti-RHOBTB1 antibody [EPR8990] (AB150408)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise