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AB92876

Anti-Ribonuclease H2, subunit A/RNASEH2A antibody

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(4 제품이 사용된 논문 )

Mouse Polyclonal Ribonuclease H2, subunit A/RNASEH2A antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RNASEH2A.
4 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB92876)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB92876)

ab92876 at 10μg/ml staining Ribonuclease H2, subunit A/RNASEH2A on Hela cell by immunofluorescence

Western blot - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB92876)
  • WB

Unknown

Western blot - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB92876)

All lanes:

Western blot - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab92876) at 1/500 dilution

All lanes:

Jurkat cell lysate at 50 µg

Predicted band size: 33 kDa

false

Western blot - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB92876)
  • WB

Unknown

Western blot - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB92876)

All lanes:

Western blot - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab92876) at 1/500 dilution

Lane 1:

Ribonuclease H2, subunit A/RNASEH2A transfected 293T cell lysate at 50 µg

Lane 2:

Non-transfected cell line. at 50 µg

Predicted band size: 33 kDa

false

Western blot - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB92876)
  • WB

Unknown

Western blot - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB92876)

All lanes:

Western blot - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (ab92876) at 1/500 dilution

All lanes:

COLO 320 HSR at 50 µg

Predicted band size: 33 kDa

false

주요 정보

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RNASEH2A.

O75792

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대체품

Primary Antibodies

AB83943

Anti-Ribonuclease H2, subunit A/RNASEH2A antibody

Immunocytochemistry - Anti-Ribonuclease H2, subunit A/RNASEH2A antibody (AB83943)

  • 0
  • 0

보완 제품

Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

  • 5
  • 104
Primary Antibodies

AB229078

Anti-RNase H1/RNH1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNase H1/RNH1 antibody (AB229078)

  • 0
  • 0
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB272067

Anti-53BP1 antibody [BL-250-1H11] - BSA free

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody [BL-250-1H11] - BSA free (AB272067)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ribonuclease H2 subunit A also known as RNASEH2A is one part of the RNase H2 enzyme complex. This protein weighs approximately 33 kilodaltons and plays an important role in RNA-DNA hybrid processing. RNASEH2A operates by cleaving the RNA strand of RNA-DNA hybrids which is necessary for maintaining genome stability. The protein expresses in a variety of tissues. Importantly it is found in high levels in cells with rapid division like embryonic and stem cells.
Biological function summary

RNASEH2A facilitates the removal of ribonucleotides from DNA which helps in DNA replication and repair. As a part of the RNase H2 complex alongside subunits B and C it ensures the correct removal of embedded ribonucleotides from DNA strands. This process is important for maintaining the integrity of the genome and preventing mutations. The activity of RNASEH2A directly impacts DNA replication fidelity and overall genome stability.

Pathways

RNASEH2A plays an important role in the maintenance of genome integrity and the DNA repair pathway. It directly interacts with the process of ribonucleotide excision repair which prevents genomic instability. RNASEH2A works alongside enzymes involved in DNA replication and repair mechanisms like DNA polymerases to correct DNA strands. The collaboration of these proteins ensures the accuracy of genomic information passed during cell division.

RNASEH2A is associated with Aicardi-Goutières syndrome a severe neurodevelopmental disorder. This syndrome results from mutations in any of the RNase H2 subunits including RNASEH2A leading to accumulated DNA damage and a chronic interferon response. Another condition linked to malfunctions in this protein is systemic lupus erythematosus where an abnormal immune response attacks the body’s own cells. Both conditions connect RNASEH2A with the regulation of immune responses and highlight its role in preventing inappropriate autoimmunity.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA : DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA : RNA duplexes.
See full target information RNASEH2A

대체 명칭 보기

RNASEHI, RNHIA, RNASEH2A, Ribonuclease H2 subunit A, RNase H2 subunit A, Aicardi-Goutieres syndrome 4 protein, RNase H(35), Ribonuclease HI large subunit, Ribonuclease HI subunit A, AGS4, RNase HI large subunit
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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular cell 81:4440-4456.e7 PubMed34597596

2021

The Protexin complex counters resection on stalled forks to promote homologous recombination and crosslink repair.

Applications

Unspecified application

Species

Unspecified reactive species

Richard O Adeyemi,Nicholas A Willis,Andrew E H Elia,Connor Clairmont,Shibo Li,Xiaohua Wu,Alan D D'Andrea,Ralph Scully,Stephen J Elledge

Nature 559:285-289 PubMed29973717

2018

CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Zimmermann,Olga Murina,Martin A M Reijns,Angelo Agathanggelou,Rachel Challis,Žygimantė Tarnauskaitė,Morwenna Muir,Adeline Fluteau,Michael Aregger,Andrea McEwan,Wei Yuan,Matthew Clarke,Maryou B Lambros,Shankara Paneesha,Paul Moss,Megha Chandrashekhar,Stephane Angers,Jason Moffat,Valerie G Brunton,Traver Hart,Johann de Bono,Tatjana Stankovic,Andrew P Jackson,Daniel Durocher

The Journal of biological chemistry 287:14280-8 PubMed22383524

2012

Frequent incorporation of ribonucleotides during HIV-1 reverse transcription and their attenuated repair in macrophages.

Applications

WB

Species

Unspecified reactive species

Edward M Kennedy,Sarah M Amie,Robert A Bambara,Baek Kim

Molecular cell 44:966-77 PubMed22195969

2011

Collisions between replication and transcription complexes cause common fragile site instability at the longest human genes.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Helmrich,Monica Ballarino,Laszlo Tora
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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