AB240597

Anti-Ribophorin I antibody

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Rabbit Polyclonal Ribophorin I antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 aa 550 to C-terminus.

대체 명칭 보기

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, Ribophorin I, Ribophorin-1, RPN-I, RPN1

1 이미지

Supplier Data

Western blot - Anti-Ribophorin I antibody (AB240597)

All lanes:

Western blot - Anti-Ribophorin I antibody (ab240597) at 0.4 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 69 kDa

false

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P04843

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ribophorin I also known as RPN1 is a glycoprotein with a mass of approximately 68 kDa. It is located in the rough endoplasmic reticulum (ER) and is part of the oligosaccharyltransferase complex which is involved in the N-linked glycosylation of nascent polypeptides. This protein facilitates the unanimous transfer of oligosaccharides to asparagine residues in polypeptide chains an important step in protein maturation. Ribophorin I shows expression in various tissues but is especially prominent in the liver and pancreas where high levels of protein synthesis occur.

Biological function summary

Ribophorin I plays an essential role in the process of protein folding and quality control within the ER. It is part of the oligosaccharyltransferase complex along with other key proteins like STT3A/B and Ribophorin II. The complex ensures that nascent proteins receive the proper glycosylation necessary for proper folding and stability. This process helps maintain a balance between protein production and degradation ensuring that only properly folded proteins proceed to their intended cellular locations.

Pathways

Ribophorin I functions within the N-linked glycosylation pathway critical for protein maturation and function. Also it takes part in the unfolded protein response pathway which reacts to stress conditions that disrupt the folding processes in the ER. This pathway involves other proteins such as BiP/GRP78 and ATF6 which work together to restore normal protein folding conditions. Ribophorin I's efficient function in these pathways is key to sustaining cellular operations maintaining protein homeostasis.

Ribophorin I is implicated in cancer and neurodegenerative diseases. Its malfunction can disrupt protein glycosylation and folding contributing to the progression of cancer by allowing aberrant glycoproteins on the cell surface. In neurodegenerative disorders like Alzheimer's disease impaired N-linked glycosylation affects protein folding leading to aggregation of misfolded proteins. Ribophorin I interacts indirectly with proteins such as amyloid precursor protein (APP) in Alzheimer's influencing the disease pathology through related glycosylation processes.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed : 31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity).

See full target information Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Heliyon 10:e39260 PubMed39669138

2024

Systematic exploration of the molecular characteristics of CD8 T cells to predict the response to immunotherapy and the prognosis of patients with colon adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Shen,Lifeng Shang,Junwei Han,Yi Zhang,Wenkai Niu,Haiwang Liu,Hai Shi

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ribophorin I antibody

Western blot - Anti-Ribophorin I antibody (AB240597)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise