AB224447

Anti-RIDA antibody

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Rabbit Polyclonal RIDA antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RIDA aa 50 to C-terminus.

대체 명칭 보기

HRSP12, RIDA, 2-iminobutanoate/2-iminopropanoate deaminase, 14.5 kDa translational inhibitor protein, Heat-responsive protein 12, Reactive intermediate imine deaminase A homolog, Translation inhibitor L-PSP ribonuclease, UK114 antigen homolog, hp14.5, p14.5

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)

Paraffin-embedded human kidney stained for RIDA using ab224447 at 1/1000 dilution in immunohistochemical analysis

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)

Paraffin-embedded human liver stained for RIDA using ab224447 at 1/1000 dilution in immunohistochemical analysis

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)

Paraffin-embedded human Fallopian tube stained for RIDA using ab224447 at 1/1000 dilution in immunohistochemical analysis

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)

Paraffin-embedded human skin stained for RIDA using ab224447 at 1/1000 dilution in immunohistochemical analysis

Supplier Data

Western blot - Anti-RIDA antibody (AB224447)

All lanes:

Western blot - Anti-RIDA antibody (ab224447) at 1/100 dilution

Lane 1:

Mouse liver lysate

Lane 2:

Rat liver lysate

Predicted band size: 14 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RIDA aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P52758

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RIDA also known as Reactive Intermediate Deaminase A functions as an important enzyme in cellular processes. This protein has a molecular weight of approximately 19 kDa and is prominently expressed in various tissues including the liver and kidneys. RIDA acts by deaminating reactive intermediates specifically 2-aminoacrylate which in turn prevents toxic accumulation within cells. Its expression sees influence under various metabolic stress conditions indicating its important role in maintaining cellular homeostasis.

Biological function summary

Reactive intermediate deaminase A supports metabolic resilience by engaging in the nitrogen metabolism pathway. It functions as part of a deaminase complex important for processing amino acid intermediates. During this metabolic function RIDA catalyzes the conversion of potentially toxic compounds into less harmful ones facilitating their subsequent expulsion or further processing. Such activity helps maintain the balance of available nitrogenous compounds in the cell which is necessary for proper cellular function.

Pathways

Reactive intermediate deaminase A integrates into the amino acid metabolism and the central nitrogen metabolism pathways. These pathways involve several interactive proteins like serine hydroxymethyltransferase supporting the correct regulation and maintenance of cellular nitrogen balance. RIDA collaborates with these proteins to ensure that amino acid degradation is smooth and without the buildup of intermediate byproducts.

Some studies associate reactive intermediate deaminase A with metabolic disorders involving amino acid imbalances. Diseases such as hyperoxaluria and nonketotic hyperglycinemia show altered activities of proteins connected to RIDA indicating its potential role in these conditions. The connection between RIDA and other proteins like glycine decarboxylase suggests a broader impact on the intricate balance of amino acids in the human body.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the hydrolytic deamination of enamine/imine intermediates that form during the course of normal metabolism. May facilitate the release of ammonia from these potentially toxic reactive metabolites, reducing their impact on cellular components. It may act on enamine/imine intermediates formed by several types of pyridoxal-5'-phosphate-dependent dehydratases including L-threonine dehydratase.. Also promotes endoribonucleolytic cleavage of some transcripts by promoting recruitment of the ribonuclease P/MRP complex (PubMed : 30930054, PubMed : 8973653). Acts by bridging YTHDF2 and the ribonuclease P/MRP complex (PubMed : 30930054). RIDA/HRSP12 binds to N6-methyladenosine (m6A)-containing mRNAs containing a 5'-GGUUC-3' motif : cooperative binding of RIDA/HRSP12 and YTHDF2 to such transcripts lead to recruitment of the ribonuclease P/MRP complex and subsequent endoribonucleolytic cleavage (PubMed : 30930054).

See full target information RIDA

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Histology and histopathology 39:877-887 PubMed37750664

2023

Selenomethionine suppresses head and neck squamous cell carcinoma progression through TopBP1/ATR and TCAB1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Xiaodong Wei,Jiwu Li

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RIDA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIDA antibody (AB224447)

Western blot - Anti-RIDA antibody (AB224447)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise