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AB229632

Anti-Rif1 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal RIF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Telomere-associated protein RIF1.

대체 명칭 보기

Telomere-associated protein RIF1, Rap1-interacting factor 1 homolog, RIF1

1 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Rif1 antibody (AB229632)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Rif1 antibody (AB229632)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Rif1 (green) using ab229632 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain : Hoechst 33342 (blue) and phalloidin, a cytoskeleton marker (red).

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Telomere-associated protein RIF1. The exact immunogen used to generate this antibody is proprietary information.

Q5UIP0

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대체품

Primary Antibodies

AB229656

Anti-Rif1 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody - C-terminal (AB229656)

  • 0
  • 0
Primary Antibodies

AB13422

Anti-Rif1 antibody

Western blot - Anti-Rif1 antibody (AB13422)

  • 3
  • 1
Primary Antibodies

AB70254

Anti-Rif1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody (AB70254)

  • 0
  • 0

보완 제품

Primary Antibodies

AB170104

Anti-NELFe antibody [EPR11600]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NELFe antibody [EPR11600] (AB170104)

  • 5
  • 1
Primary Antibodies

AB133534

Anti-Rad51 antibody [EPR4030(3)]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (AB133534)

  • 4
  • 15
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB239074

Anti-STING antibody [EPR13130-55]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STING antibody [EPR13130-55] (AB239074)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Rif1 also known as Rap1-interacting factor 1 is a protein that aids in DNA end protection and repair processes. The protein has a molecular mass of approximately 240 kDa and is predominantly expressed in the nucleus of eukaryotic cells. Rif1 interacts directly with telomeric DNA and regulates DNA-double strand break (DSB) repair. Its activity ensures genomic integrity by coordinating access to damaged DNA ends which is essential in the context of maintaining telomere length and chromosomal stability.
Biological function summary

Rif1 plays a central role in the DNA damage response and DNA replication. It operates as part of the shieldin complex which participates in non-homologous end joining (NHEJ) by protecting DNA ends from extensive resection. By doing so Rif1 directs the repair pathway choice towards NHEJ over homologous recombination enabling cell cycle progression in conditions of DNA stress. Its function is particularly significant during the S and G1 phases of the cell cycle during which Rif1 modulates replication timing and forks stalling recovery.

Pathways

Several critical cellular processes involve Rif1 particularly DNA damage repair and cell cycle regulation. Within these pathways Rif1 interacts with proteins such as 53BP1 which is instrumental in controlling pathway selectivity during DNA repair and it influences origin firing through its interplay with replication timing guards. Additionally Rif1 works in conjunction with ATM and ATR kinases which are key regulators in the phosphorylation cascade triggered by DNA damage. These interactions underpin Rif1’s pivotal role in cell cycle checkpoints and repair signaling pathways.

Aberrations in Rif1 function have links to cancer and premature aging diseases. Altered Rif1 expression or malfunction can contribute to tumorigenesis especially in cancers where DNA repair pathways are disrupted. This is due in part to its relationship with 53BP1 playing roles in tumor suppression pathways by maintaining genomic stability. Furthermore mutations affecting Rif1 are associated with conditions like Hutchinson-Gilford Progeria Syndrome. This connection to lamin A/C another protein involved in nuclear stability highlights its broader impact on maintaining cellular homeostasis and genome integrity.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage : acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs (PubMed : 15342490, PubMed : 28241136). In response to DNA damage, interacts with ATM-phosphorylated TP53BP1 (PubMed : 23333306, PubMed : 28241136). Interaction with TP53BP1 leads to dissociate the interaction between NUDT16L1/TIRR and TP53BP1, thereby unmasking the tandem Tudor-like domain of TP53BP1 and allowing recruitment to DNA DSBs (PubMed : 28241136). Once recruited to DSBs, RIF1 and TP53BP1 act by promoting NHEJ-mediated repair of DSBs (PubMed : 23333306). In the same time, RIF1 and TP53BP1 specifically counteract the function of BRCA1 by blocking DSBs resection via homologous recombination (HR) during G1 phase (PubMed : 23333306). Also required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (By similarity). Promotes NHEJ of dysfunctional telomeres (By similarity).
See full target information Telomere-associated protein RIF1
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in oncology 14:1302850 PubMed38420012

2024

Bevacizumab increases the sensitivity of olaparib to homologous recombination-proficient ovarian cancer by suppressing CRY1 via PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yasushi Iida,Nozomu Yanaihara,Yuki Yoshino,Misato Saito,Ryosuke Saito,Junya Tabata,Ayako Kawabata,Masataka Takenaka,Natsuko Chiba,Aikou Okamoto

Frontiers in oncology 12:763480 PubMed36110943

2022

La protein regulates protein expression by binding with the mRNAs of target genes and participates the pathological process of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Huang,Jialei Zhu,Yueyan Li,Yang Yu,Jing Tang
제품이 사용된 논문 모두 보기
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Product promise

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