JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325011

Anti-Rift Valley Fever Virus Nucleoprotein antibody [AA11]

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Mouse Recombinant Monoclonal NCAP antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Rift valley fever virus (STRAIN ZH-548 M12), Rift valley fever virus (STRAIN ZH-548 M12) samples.
2 이미지
Indirect ELISA - Anti-Rift Valley Fever Virus Nucleoprotein antibody [AA11] (AB325011)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Rift Valley Fever Virus Nucleoprotein antibody [AA11] (AB325011)

Indirect ELISA analysis of ab325011 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution dilution.

Antigen : Rift Valley Fever Virus Nucleoprotein.

Antigen concentration : 1000 ng/ml

Western blot - Anti-Rift Valley Fever Virus Nucleoprotein antibody [AA11] (AB325011)
  • WB

Supplier Data

Western blot - Anti-Rift Valley Fever Virus Nucleoprotein antibody [AA11] (AB325011)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Rift Valley Fever Virus Nucleoprotein antibody [AA11] (ab325011) at 1/1000 dilution

Lane 1:

293T cells transfected with Rift valley fever virus (RVFV) Nucleoprotein (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 1 µg

Lane 2:

293T cells transfected with Rift valley fever virus (RVFV) Envelopment polyprotein (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with Rift valley fever virus (RVFV) RNA-directed RNA polymerase L (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with Rift valley fever virus (RVFV) Non-structural protein S (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 5 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

Observed band size: 28-243 kDa,36 kDa

false

Exposure time: 180s

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

AA11

Isotype

IgG2a

Carrier free

No

Reacts with

Rift valley fever virus (STRAIN ZH-548 M12)

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

보완 제품

Primary Antibodies

AB283946

Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174]

20ul selling size|RabMAb|Recombinant

Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (AB283946)

  • 0
  • 1
Primary Antibodies

AB320652

Anti-hMPV fusion Protein antibody [EPR29365-579]

20ul selling size|Recombinant|RabMAb

Immunocytochemistry/ Immunofluorescence - Anti-hMPV fusion Protein antibody [EPR29365-579] (AB320652)

  • 0
  • 0
Primary Antibodies

AB183497

Anti-VSV-G tag antibody [EPR12997]

RabMAb|Recombinant|Lab Essentials

Western blot - Anti-VSV-G tag antibody [EPR12997] (AB183497)

  • 4
  • 2
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rift valley fever virus (STRAIN ZH-548 M12)": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "31.25 ng/mL", "IELISA-species-notes": "<p></p>" }, "Transfected cell lysate - Rift valley fever virus (STRAIN ZH-548 M12)": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
style
left-column

제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Encapsidates the genomic RNA, protecting it from nucleases (By similarity). Displays high affinity for single-stranded nucleic acid (By similarity). The encapsidated genomic RNA is termed the nucleocapsid (NC) (By similarity). The ribonucleoprotein has a non-helical structure (By similarity). Serves as template for viral transcription and replication (PubMed : 7769655). After replication, the nucleocapsid is recruited to the host Golgi apparatus by glycoprotein Gn for packaging into virus particles (PubMed : 21445316).
See full target information N

대체 명칭 보기

Nucleoprotein, Nucleocapsid protein, Protein N
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com