AB241361

Anti-RIOK3 antibody

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Rabbit Polyclonal RIOK3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RIOK3 aa 450 to C-terminus.

대체 명칭 보기

SUDD, RIOK3, Serine/threonine-protein kinase RIO3, RIO kinase 3, sudD homolog

2 이미지

Supplier Data

Immunoprecipitation - Anti-RIOK3 antibody (AB241361)

RIOK3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241361 at 20 μl/reaction. Western blot was performed from the immunoprecipitate using ab241361 at 1/250 dilution.

Lane 1 : ab241361 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysate prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-RIOK3 antibody (ab241361)

Predicted band size: 59 kDa

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Supplier Data

Western blot - Anti-RIOK3 antibody (AB241361)

All lanes:

Western blot - Anti-RIOK3 antibody (ab241361) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Predicted band size: 59 kDa

true

Exposure time: 75s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RIOK3 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O14730

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use 20 μl/mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab241361 was affinity purified using an epitope specific to RIOK3 immobilized on solid support.

보관 버퍼

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RIOK3 also known as RIO kinase 3 or SUDD is a serine/threonine kinase with a molecular mass of approximately 65 kDa. This protein acts as an atypical kinase within the RIO kinase family and localizes to the cytoplasm. RIOK3 shows expression in various tissues with higher levels notably in the immune system including the spleen and thymus. Unlike typical kinases RIOK3 contains a unique winged-helix domain that contributes to its diverse functions in cellular processes.

Biological function summary

RIOK3 participates in immune response regulation and cellular signal transduction. It does not form part of any known large protein complex but interacts with several other proteins through transient associations. By modulating pathways involved in immune processes RIOK3 affects the activation state of various immune cells. It is involved in the regulation of interferon production where it works closely with other signaling proteins to modulate responses to viral infections.

Pathways

RIOK3 serves a role in the innate immune response and RNA processing pathways. It influences the retinoic acid-inducible gene I (RIG-I) pathway where it interacts with proteins such as RIG-I and MAVS to mediate antiviral responses. Additionally RIOK3 plays a modulatory role in mRNA processing connecting with proteins involved in RNA maturation and internationalization which suggests its involvement in maintaining proper RNA homeostasis under stress conditions.

RIOK3 has associations with autoimmune conditions and viral infections highlighting its role in immune regulation. Aberrant functioning of RIOK3 can relate to systemic lupus erythematosus (SLE) where it influences the overproduction of interferons exacerbating the disease phenotype. Furthermore RIOK3 modulates responses to hepatitis C virus infections interacting with proteins such as RIG-I to control viral replication and immune evasion. Understanding RIOK3's functions can lead to insights into therapeutic interventions for these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Serine/threonine-protein kinase involved in a ribosome quality control that takes place when ribosomes have stalled, leading to 18S non-functional rRNA decay and degradation of the 40S ribosomal subunit (PubMed : 39947182, PubMed : 39947183, PubMed : 40022732). Acts downstream of RNF10 : specifically recognizes and binds RPS2/us5 and RPS3/us3 monoubiquitinated by RNF10, promoting degradation of the 40S ribosomal subunit in a kinase-dependent manner (PubMed : 39947182, PubMed : 39947183, PubMed : 40022732). The RNF10-RIOK3 ribosome quality control takes place in response to ribosome subunit imbalance or downstream the EIF2AK4/GCN2-mediated integrated stress response (ISR) (PubMed : 39947182, PubMed : 39947183, PubMed : 40022732). Also involved in regulation of type I interferon (IFN)-dependent immune response, possibly by acting as an adapter protein essential for the recruitment of TBK1 to IRF3 (PubMed : 24807708). Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling (PubMed : 25865883). Can inhibit CASP10 isoform 7-mediated activation of the NF-kappa-B signaling pathway (PubMed : 19557502).

See full target information RIOK3

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death discovery 7:392 PubMed34907164

2021

CRISPR/Cas9 genome-wide screening identifies LUC7L2 that promotes radioresistance via autophagy in nasopharyngeal carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Shen,Chao Li,Fang Chen,Liangfang Shen,Zhanzhan Li,Na Li

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RIOK3 antibody

Immunoprecipitation - Anti-RIOK3 antibody (AB241361)

Western blot - Anti-RIOK3 antibody (AB241361)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Proteins & Peptides

Biochemicals

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise