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AB191598

Anti-RNA polymerase beta antibody [EPR18704]

5

(2 리뷰들)

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(14 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal RNA polymerase beta antibody. Suitable for WB, IP and reacts with Escherichia coli samples. Cited in 14 publications.

대체 명칭 보기

groN, nitB, rif, ron, stl, stv, tabD, b3987, JW3950, rpoB, DNA-directed RNA polymerase subunit beta, RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta, rif, ron, stl, stv, groN, nitB, rpoB, tabD, RNA pol beta, RNAP subunit beta, Transcriptase subunit beta, RNA polymerase subunit beta, RNA polymerase, beta subunit

2 이미지
Immunoprecipitation - Anti-RNA polymerase beta antibody [EPR18704] (AB191598)
  • IP

Supplier Data

Immunoprecipitation - Anti-RNA polymerase beta antibody [EPR18704] (AB191598)

RNA polymerase beta was immunoprecipitated from 1mg of E.coli whole cell lysates with ab191598 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191598 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Lane 1 : E.coli whole cell lysates 10ug (Input). Lane 2 : ab191598 IP in E.coli whole cell lysates. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab191598 in E.coli whole cell lysates.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-RNA polymerase beta antibody [EPR18704] (ab191598)

false

Western blot - Anti-RNA polymerase beta antibody [EPR18704] (AB191598)
  • WB

Supplier Data

Western blot - Anti-RNA polymerase beta antibody [EPR18704] (AB191598)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RNA polymerase beta antibody [EPR18704] (ab191598) at 1/2000 dilution

All lanes:

E.coli whole cell lysates at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 150 kDa

Observed band size: 150 kDa

false

Exposure time: 30s

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-RNA polymerase beta antibody [EPR18704] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18704

Isotype

IgG

Carrier free

No

Reacts with

Escherichia coli

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB82592

Anti-groEL antibody [9A1/2]

Western blot - Anti-groEL antibody [9A1/2] (AB82592)

  • 0
  • 0
Primary Antibodies

AB69617

Anti-DnaK antibody [8E2/2]

Western blot - Anti-DnaK antibody [8E2/2] (AB69617)

  • 4
  • 2
Primary Antibodies

AB63797

Anti-RecA antibody

Western blot - Anti-RecA antibody (AB63797)

  • 5
  • 4
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RNA polymerase beta also known as RpoB or DNA-directed RNA polymerase subunit beta is a critical enzyme in the transcription process. This protein has a molecular mass of approximately 150 kDa. RpoB plays a role by catalyzing the synthesis of RNA from a DNA template an essential step in gene expression. It is widely expressed in bacteria mitochondria chloroplasts and also in the nuclei of eukaryotic cells. RpoB functions as a fundamental part of the core RNA polymerase complex which also includes other subunits such as alpha beta' and omega.
Biological function summary

RpoB interacts with multiple subunits to form the RNA polymerase holoenzyme integrating into cellular machinery to initiate transcription. This enzyme participates in synthesizing mRNA rRNA and tRNA all of which are important for protein synthesis. The holoenzyme formation aligns with promoter regions on DNA enabling precise transcription initiation. Without the proper function of RpoB and its associated subunits transcription efficiency and fidelity could be compromised impacting cell viability.

Pathways

RNA polymerase beta is integral to transcriptional regulation pathways and genetic response mechanisms within the cell. It plays a role in the bacterial transcription pathway directly interacting with sigma factors and other transcription regulators to manage gene expression in response to environmental changes. Furthermore RpoB operates in conjunction with other proteins like sigma factors which allow the enzyme to recognize specific promoter sequences ensuring the transcription of appropriate genes.

The improper function of RNA polymerase beta has correlations with bacterial resistance to antibiotics notably rifampicin. Mutations in the rpoB gene can lead to resistance complicating treatment strategies for conditions like tuberculosis. This enzyme's relationship with sigma factors also presents a route through which these mutations affect transcription fidelity leading to resistant bacterial strains. Understanding these connections helps to develop therapies targeting the bacterial transcription machinery effectively.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.. Resistance to the antibiotics salinamide A, salinamide B, rifampicin, streptolydigin, CBR703, myxopyronin, and lipiarmycin can result from mutations in this protein.. Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNAP. It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis.
See full target information rpoB
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제품이 사용된 논문 (14)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 16:2000 PubMed40011517

2025

Dual-function regulator MexL as a target to control phenazines production and pathogenesis of Pseudomonas aeruginosa.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoxiao Yu,Zhikun Wu,Dejian Liu,Haoyu Liu,Yu Zhang,Yaqian Zheng,Yanhong Huang,Shumin Liao,Yu Wei,Wei Huang,Zhenyu Zhang,Xi Liu,Haiying Yu,Di Wang,Liang Li,Feng Long,Luyan Z Ma

Cell host & microbe 32:1059-1073.e8 PubMed38821063

2024

Mechanism of phage sensing and restriction by toxin-antitoxin-chaperone systems.

Applications

Unspecified application

Species

Unspecified reactive species

Toomas Mets,Tatsuaki Kurata,Karin Ernits,Marcus J O Johansson,Sophie Z Craig,Gabriel Medina Evora,Jessica A Buttress,Roni Odai,Kyo Coppieters't Wallant,Jose A Nakamoto,Lena Shyrokova,Artyom A Egorov,Christopher Ross Doering,Tetiana Brodiazhenko,Michael T Laub,Tanel Tenson,Henrik Strahl,Chloe Martens,Alexander Harms,Abel Garcia-Pino,Gemma C Atkinson,Vasili Hauryliuk

eLife 13: PubMed38739431

2024

PhoP integrates stress response to intracellular survival by regulating cAMP level.

Applications

Unspecified application

Species

Unspecified reactive species

Hina Khan,Partha Paul,Harsh Goar,Bhanwar Bamniya,Navin Baid,Dibyendu Sarkar

Immunity, inflammation and disease 11:e1015 PubMed37773716

2023

Effects of albiflorin on oxidative stress and inflammatory responses in rats with acute spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Pengfei Fang,Yi Wang,Fengqi Sun,Haisheng Lin,Xindong Zhang

Journal of bacteriology 204:e0033522 PubMed36448788

2022

The Sia System and c-di-GMP Play a Crucial Role in Controlling Cell-Association of Psl in Planktonic P. aeruginosa.

Applications

Unspecified application

Species

Unspecified reactive species

Julia E Dreifus,Lindsey O'Neal,Holly M Jacobs,Adithya S Subramanian,P Lynne Howell,Daniel J Wozniak,Matthew R Parsek

Science advances 8:eabm2343 PubMed35486720

2022

N-terminal signal peptides facilitate the engineering of PVC complex as a potent protein delivery system.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Jiang,Jiawei Shen,Jiaxuan Cheng,Xia Wang,Jianguo Yang,Ningning Li,Ning Gao,Qi Jin

eLife 11: PubMed35015630

2022

Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Kaitlyn Tsai,Vanja Stojković,Lianet Noda-Garcia,Iris D Young,Alexander G Myasnikov,Jordan Kleinman,Ali Palla,Stephen N Floor,Adam Frost,James S Fraser,Dan S Tawfik,Danica Galonić Fujimori

Computational and structural biotechnology journal 19:6179-6190 PubMed34900131

2021

Bacterial quorum sensing quenching activity of leucyl aminopeptidase acts by interacting with autoinducer synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Jinxing Liao,Danyu Shen,Long Lin,Hongjun Chen,Yajie Jin,Shan-Ho Chou,Xiao-Quan Yu,Tao Li,Guoliang Qian

Molecular microbiology 116:1216-1231 PubMed34494331

2021

Bis-molybdopterin guanine dinucleotide modulates hemolysin expression under anaerobiosis and contributes to fitness in vivo in uropathogenic Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyang Zhang,Dongyan Huang,Zihui Zhao,Xuwang Cai,Wentong Cai,Ganwu Li

mSphere 6:e0059921 PubMed34346702

2021

Identification of a Family of Type III Secretion System Effectors That Contain a Conserved Serine/Threonine Kinase Domain.

Applications

Unspecified application

Species

Unspecified reactive species

N Plaza,I M Urrutia,K Garcia,M K Waldor,C J Blondel
제품이 사용된 논문 모두 보기
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Product promise

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