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AB222073

Anti-RNA polymerase II RPB4 antibody

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Rabbit Polyclonal RNA polymerase II RPB4 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human POLR2D aa 50 to C-terminus.

대체 명칭 보기

DNA-directed RNA polymerase II subunit RPB4, RNA polymerase II subunit B4, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16 kDa subunit, RPB16, POLR2D

1 이미지
Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II RPB4 antibody (AB222073)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II RPB4 antibody (AB222073)

PFA-fixed, Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for RNA polymerase II RPB4 (green) using ab222073 at 4 μg/ml in ICC/IF.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human POLR2D aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15514

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대체품

Primary Antibodies

AB229488

Anti-RNA polymerase II RPB4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II RPB4 antibody (AB229488)

  • 0
  • 0
Primary Antibodies

AB186868

Anti-RNA polymerase II RPB4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA polymerase II RPB4 antibody (AB186868)

  • 0
  • 0

보완 제품

Primary Antibodies

AB246976

Anti-POLR1C antibody

Immunocytochemistry/ Immunofluorescence - Anti-POLR1C antibody (AB246976)

  • 0
  • 0
Primary Antibodies

AB264109

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [4H8] - BSA and Azide free

Recombinant

Flow Cytometry - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [4H8] - BSA and Azide free (AB264109)

  • 0
  • 0
Primary Antibodies

AB5095

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody

Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (AB5095)

  • 4
  • 50
Primary Antibodies

AB222065

Anti-POLR1A antibody

Immunocytochemistry/ Immunofluorescence - Anti-POLR1A antibody (AB222065)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RNA polymerase II RPB4 also known by the alternate name POLR2D is a subunit of the larger RNA polymerase II enzyme complex. This enzyme plays a critical role in catalyzing the transcription of DNA into messenger RNA (mRNA) which is fundamental to protein synthesis. RPB4 is part of the core enzyme contributing to its stability and function. It has a molecular mass of approximately 25 kDa. This protein is widely expressed in eukaryotic cells particularly in the nucleus where transcription occurs.
Biological function summary

RNA polymerase II RPB4 functions as a component of the larger RNA polymerase II complex. It contributes to the regulation and initiation of mRNA synthesis. This subunit has essential roles in transcriptional regulation under stress conditions by stabilizing the RNA polymerase II complex. The interaction with co-factors and other transcription machinery highlights RPB4's involvement in adaptive responses of the cell.

Pathways

RNA polymerase II RPB4 integrates into essential transcription pathways such as the mRNA processing and the transcription initiation pathway. It cooperates with other subunits and factors like TFIIB and TFIIH to effectively facilitate the transcription process. These pathways determine the accurate transcription and downstream processes necessary for gene expression playing an interconnected role with other proteins like TAFs within the transcriptional machinery.

RNA polymerase II RPB4 is associated with several types of cancers as transcription regulation often becomes dysregulated in cancer cells. Disruptions in RPB4 expression or function can also relate to neurodegenerative disorders where transcriptional errors contribute to the disease pathology. Interaction of RPB4 with proteins like p53 may highlight its involvement in these conditions as p53 is a critical regulator of cell cycle and apoptosis often altered in cancerous states.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. POLR2D/RPB4 is part of a subcomplex with POLR2G/RPB7 that binds to a pocket formed by POLR2A/RPB1, POLR2B/RPB2 and POLR2F/RPABC2 at the base of the clamp element. The POLR2D/RPB4-POLR2G/RPB7 subcomplex seems to lock the clamp via POLR2G/RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The POLR2D/RPB4-POLR2G/RPB7 subcomplex binds single-stranded DNA and RNA.
See full target information POLR2D
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Product promise

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