Anti-RNase L antibody [EPR15894]

• WB

AbReview48801****

Western blot - Anti-RNase L antibody [EPR15894] (AB191392)

Blocked with 5% milk in TBST for 4 hours at 4°C.

Incubated with the primary antibody in TBST for 16 hours at 4°C.

PageRuler* Prestained Protein Ladder 10-170kDa (170, 130, 100, 70 (red), 55, 40, 35, 25, 15 and 10kDa).

All lanes:

Western blot - Anti-RNase L antibody [EPR15894] (ab191392) at 1/10000 dilution

Lanes 1 and 5:

HAP1 WT at 1000 Cells

Lane 2:

HAP1 RNase L KO at 1000 Cells

Lanes 3 and 7:

T47Ds at 1000 Cells

Lanes 4 and 8:

Recombinant RNase L (2 nM) at 1 µL

Lane 6:

HAP1 KO at 1000 Cells

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

Predicted band size: 84 kDa

Observed band size: 80 kDa

true

Exposure time: 2min

This image is courtesy of an anonymous Abreview

• WB

AbReview79010****

Western blot - Anti-RNase L antibody [EPR15894] (AB191392)

4-15% gel. Blocking was done using 5% milk for 1 hour at 25°C

All lanes:

Western blot - Anti-RNase L antibody [EPR15894] (ab191392) at 1/1000 dilution

All lanes:

HeLa cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated affinipure goat anti-rabbit at 1/5000 dilution

true

Exposure time: 1min

This image is courtesy of an anonymous Abreview

• WB

Supplier Data

Western blot - Anti-RNase L antibody [EPR15894] (AB191392)

All lanes:

Western blot - Anti-RNase L antibody [EPR15894] (ab191392) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Y79 cell lysate at 20 µg

Lane 3:

THP1 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 84 kDa

false