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AB74373

Anti-RNF125 antibody

4

(1 리뷰)

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(2 제품이 사용된 논문 )

Rabbit Polyclonal RNF125 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.
2 이미지
Western blot - Anti-RNF125 antibody (AB74373)
  • WB

AbReview39333****

Western blot - Anti-RNF125 antibody (AB74373)

All lanes:

Western blot - Anti-RNF125 antibody (ab74373) at 1/200 dilution

Lane 1:

293T whole cell lysate at 5 µg

Lane 2:

Mouse ES (129Sv) whole cell lysate at 5 µg

Secondary

All lanes:

HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution

Predicted band size: 26 kDa

Observed band size: 100 kDa,26 kDa

false

Exposure time: 1min

This image is courtesy of an anonymous Abreview

Western blot - Anti-RNF125 antibody (AB74373)
  • WB

Unknown

Western blot - Anti-RNF125 antibody (AB74373)

All lanes:

Western blot - Anti-RNF125 antibody (ab74373) at 1/500 dilution

Lane 1:

Extracts from K562 cells at 5 µg

Lane 2:

Extracts from HT-29 cells at 5 µg

Lane 3:

Extracts from K562 cells at 5 µg with immunising peptide

Predicted band size: 26 kDa

Observed band size: 100 kDa,23 kDa,26 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB211450

Anti-RNF125 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF125 antibody (AB211450)

  • 0
  • 0

보완 제품

Primary Antibodies

AB154862

Anti-TRIM56 antibody [EPR10583]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM56 antibody [EPR10583] (AB154862)

  • 0
  • 0
Primary Antibodies

AB200038

Anti-PCGF6 antibody [EPR19450]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PCGF6 antibody [EPR19450] (AB200038)

  • 0
  • 0
Primary Antibodies

AB54362

Anti-CBLB antibody [246C5a]

BOND RX™ Validated|KO Validated

Western blot - Anti-CBLB antibody [246C5a] (AB54362)

  • 4
  • 1
Primary Antibodies

AB182163

Anti-KLHL6 antibody [EPR13484]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-KLHL6 antibody [EPR13484] (AB182163)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RNF125 also known as TRAC-1 is a protein encoded by the RNF125 gene. It belongs to the RING finger family and functions as an E3 ubiquitin-protein ligase which attaches ubiquitin to protein substrates directing them for degradation. RNF125 has a molecular mass of approximately 51 kDa. Researchers observe its expression predominantly in immune-related tissues such as the thymus spleen and peripheral blood leukocytes.
Biological function summary

RNF125 participates in the immune response by modulating the activity and stability of key signaling proteins. It forms part of a regulatory complex that influences immune signaling pathways. RNF125 acts by ubiquitinating proteins like MAVS which play a role in antiviral responses. Through ubiquitination RNF125 helps regulate the balance between activation and suppression of immune reactions ensuring proper immune function.

Pathways

Researchers have identified RNF125 as an important component of the type I interferon signaling pathway and the TLR (Toll-like receptor) pathway. In the type I interferon pathway RNF125 targets proteins such as MAVS and RIG-I playing a role in antiviral defense. Furthermore RNF125 interacts with TRAF6 in the TLR pathway influencing the body's innate immunity and inflammation responses.

RNF125 has connections to immune-related conditions such as autoimmune diseases and chronic viral infections. For example aberrant regulation of RNF125 activity might contribute to systemic lupus erythematosus (SLE) through improper control of MAVS and other immune signaling proteins. Moreover in the context of chronic viral infections RNF125's role in targeting RIG-I for degradation could hinder the immune system's ability to clear persistent viral infections. Understanding these associations provides insights into potential therapeutic approaches.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as RIGI, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53 (PubMed : 15843525, PubMed : 17460044, PubMed : 17643463, PubMed : 25591766, PubMed : 26027934, PubMed : 26471729, PubMed : 27411375). Acts as a negative regulator of type I interferon production by mediating ubiquitination of RIGI at 'Lys-181', leading to RIGI degradation (PubMed : 17460044, PubMed : 26471729). Mediates ubiquitination and subsequent degradation of p53/TP53 (PubMed : 25591766). Mediates ubiquitination and subsequent degradation of JAK1 (PubMed : 26027934). Acts as a positive regulator of T-cell activation (PubMed : 15843525).
See full target information RNF125

대체 명칭 보기

E3 ubiquitin-protein ligase RNF125, RING finger protein 125, T-cell RING activation protein 1, TRAC-1, RNF125
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 10:926 PubMed30804369

2019

Cellular TRIM33 restrains HIV-1 infection by targeting viral integrase for proteasomal degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Hashim Ali,Miguel Mano,Luca Braga,Asma Naseem,Bruna Marini,Diem My Vu,Chiara Collesi,Germana Meroni,Marina Lusic,Mauro Giacca

Journal of immunology (Baltimore, Md. : 1950) 191:660-9 PubMed23772026

2013

Human bocavirus VP2 upregulates IFN-β pathway by inhibiting ring finger protein 125-mediated ubiquitination of retinoic acid-inducible gene-I.

Applications

Unspecified application

Species

Unspecified reactive species

Huanle Luo,Zhenfeng Zhang,Zhenhua Zheng,Xianliang Ke,Xiaowei Zhang,Qian Li,Yan Liu,Bingke Bai,Panyong Mao,Qinxue Hu,Hanzhong Wang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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