AB72533

Anti-RNF128/GRAIL antibody

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(4 제품이 사용된 논문 )

Rabbit Polyclonal RNF128/GRAIL antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human E3 ubiquitin-protein ligase RNF128 aa 200-300.

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF128/GRAIL antibody (AB72533)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF128/GRAIL antibody (AB72533)

Immunohistochemical staining of paraffin-embedded normal human brain tissue using ab72533 at 5 ug/ml (Panel A) and control rabbit Ig at 5 ug/ml (Panel B).

Unknown

Western blot - Anti-RNF128/GRAIL antibody (AB72533)

Endogenous RNF128 / GRAIL is reported to be detected at approximately 66 kD

All lanes:

Western blot - Anti-RNF128/GRAIL antibody (ab72533) at 1 µg/mL

Lane 1:

human spleen lysate

Lane 2:

human spleen lysate with immunising peptide

Lane 3:

mouse spleen lysate

Lane 4:

rat spleen lysate

Predicted band size: 47 kDa

Observed band size: 25 kDa,75 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human E3 ubiquitin-protein ligase RNF128 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q8TEB7

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Opossum": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.2% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RNF128 also known as Gene Related to Anergy in Lymphocytes (GRAIL) is an E3 ubiquitin ligase with a molecular mass of approximately 44 kDa. This protein plays a mechanical role in tagging proteins with ubiquitin marking them for degradation by the proteasome. RNF128 is expressed in various tissues but it shows significant expression in immune cells particularly T cells. The protein contributes to regulating protein stability which is essential for maintaining cellular homeostasis and proper immune function.

Biological function summary

RNF128 is integral in regulating T cell anergy a state where T cells become unresponsive to antigenic stimulation. This protein helps maintain immune tolerance by suppressing excessive T cell responses preventing autoimmune reactions. RNF128 performs its biological function by being part of a complex that modulates signaling pathways involved in lymphocyte activation. Its expression and activity are modulated during immune responses regulating immune cell function and maintaining balance within the immune system.

Pathways

RNF128 is significantly involved in the ubiquitin-proteasome pathway impacting signal transduction and transcription processes. This protein is relevant in pathways related to immune tolerance particularly in controlling T cell receptor signaling. RNF128 interacts with other proteins like CD28 and CTLA-4 which are critical to T cell co-stimulatory and inhibitory pathways respectively. This interaction ensures the fine-tuning of immune responses preventing inappropriate activation that can lead to pathological states.

RNF128 has associations with autoimmune diseases and cancer. Its role in regulating immune tolerance links it to conditions like systemic lupus erythematosus where dysregulation can lead to an exaggerated immune response. Additionally RNF128's involvement in modulating T cell inactivity makes it relevant in certain cancers where immune evasion plays a critical role in disease progression. Through these conditions RNF128 remains connected with proteins like PD-1 which are involved in inhibitory pathways important for maintaining immune homeostasis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

E3 ubiquitin-protein ligase that catalyzes 'Lys-27', 'Lys-48'- or 'Lys-63'-linked polyubiquitin chains formation and plays a role in different biological processes such as modulation of immune response, cytoskeletal dynamics or protein homeostasis. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production (PubMed : 12705856). Ubiquitinates ARPC5 with 'Lys-48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytoskeletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse (PubMed : 22016387). Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals. Plays a positive role in innate immune response by promoting 'Lys-63'-linked ubiquitination of TBK1 after RNA- or DNA-virus infection (PubMed : 27776110). Regulates alveolar macrophage activation and neutrophil infiltration by interacting with TLR4, targeting it for degradation, and inhibiting NF-kappa-B activation, hence decreasing pro-inflammatory cytokines (PubMed : 37344492). Negatively regulates the IL-3/STAT5 signaling pathway by facilitating 'Lys-27'-linked polyubiquitination of IL3RA leading to its degradation via lysosomal pathway (PubMed : 38702781). Directly regulates the N-glycosylation process in the endoplasmic reticulum by targeting the glycosyl-transferase RPN1 for ubiquitination and degradation (PubMed : 39567208). Other substrates targeted for degradation by RNF128 include transmembrane proteins CD40L, CD83 or the tetraspanin CD151 (PubMed : 18713730, PubMed : 19542455).

See full target information E3 ubiquitin-protein ligase RNF128

대체 명칭 보기

E3 ubiquitin-protein ligase RNF128, Gene related to anergy in lymphocytes protein, RING finger protein 128, RING-type E3 ubiquitin transferase RNF128, GRAIL, RNF128

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death discovery 11:187 PubMed40253377

2025

RNF128 promotes gastric cancer progression by inhibiting autophagy-dependent ferroptosis through Beclin1 ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenguo Zhu,Qishuai Chen,Siyi Song,Shi Peng,Huanxin Ding,Bingjun Li,Chuxuan Liu,Xin Jin,Linchuan Li,Jiankang Zhu,Guangyong Zhang

Immunity, inflammation and disease 11:e864 PubMed37249301

2023

RNF182 induces p65 ubiquitination to affect PDL1 transcription and suppress immune evasion in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xingdu Zeng,Xiaoyuan Tang,Xingxiang Chen,Huilan Wen

OncoTargets and therapy 13:10129-10141 PubMed33116595

2020

RNF128 Promotes Malignant Behaviors via EGFR/MEK/ERK Pathway in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Song Bai,Chi Zhang,Rui Peng,Guo-Qing Jiang,Sheng-Jie Jin,Qian Wang,Ai-Wu Ke,Dou-Sheng Bai

Translational oncology 11:116-124 PubMed29223109

2017

Genome-Wide Analysis of Barrett's Adenocarcinoma. A First Step Towards Identifying Patients at Risk and Developing Therapeutic Paths.

Applications

Unspecified application

Species

Unspecified reactive species

Yiyang Dai,Qing Wang,Adrian Gonzalez Lopez,Mario Anders,Peter Malfertheiner,Michael Vieth,Wolfgang Kemmner

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RNF128/GRAIL antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF128/GRAIL antibody (AB72533)

Western blot - Anti-RNF128/GRAIL antibody (AB72533)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (4)

Product promise